Extracellular vesicles (EV) are tiny cell fragments that cells in our body use to communicate with each other and are present in bodily fluids such as blood and urine. The recent unfolding of EV as potential tools to help prevent, diagnose and even treat different diseases, such as cancer, resulted in a multitude of protocols, consider it as cookbook recipes, to isolate and analyze EV. Here, things go wrong. Experimental parameters vary between research groups and unless adequately reported, the recipes lack the knowledge to interpret and reproduce experiments.
An international consortium led by the research team of Dr. An Hendrix from Ghent University created a knowledgebank to coach researchers in the correct reporting of these recipes. The results are published in the leading journal Nature Methods.
The knowledgebank, named EV-TRACK, captures technical and biological features of EV and EV-related isolation and characterization recipes (www.evtrack.org) and informs researchers about (in)complete reporting of these recipes. As such EV-TRACK aims to accelerate breakthroughs in EV research by increasing its reproducibility at the benefit of scientists and the society.
Materials provided by Ghent University. Note: Content may be edited for style and length.
- Jan Van Deun, Pieter Mestdagh, Patrizia Agostinis, Özden Akay, Sushma Anand, Jasper Anckaert, Zoraida Andreu Martinez, Tine Baetens, Els Beghein, Laurence Bertier, Geert Berx, Janneke Boere, Stephanie Boukouris, Michel Bremer, Dominik Buschmann, James B Byrd, Clara Casert, Lesley Cheng, Anna Cmoch, Delphine Daveloose, Eva De Smedt, Seyma Demirsoy, Victoria Depoorter, Bert Dhondt, Tom A P Driedonks, Aleksandra Dudek, Abdou Elsharawy, Ilaria Floris, Andrew D Foers, Kathrin Gärtner, Abhishek D Garg, Edward Geeurickx, Jan Gettemans, Farzaneh Ghazavi, Bernd Giebel, Tom Groot Kormelink, Grace Hancock, Hetty Helsmoortel, Andrew F Hill, Vincent Hyenne, Hina Kalra, David Kim, Joanna Kowal, Sandra Kraemer, Petra Leidinger, Carina Leonelli, Yaxuan Liang, Lien Lippens, Shu Liu, Alessandra Lo Cicero, Shaun Martin, Suresh Mathivanan, Prabhu Mathiyalagan, Támas Matusek, Gloria Milani, Marta Monguió-Tortajada, Liselot M Mus, Dillon C Muth, Andrea Németh, Esther N M Nolte-'t Hoen, Lorraine O'Driscoll, Roberta Palmulli, Michael W Pfaffl, Bjarke Primdal-Bengtson, Erminia Romano, Quentin Rousseau, Susmita Sahoo, Natalia Sampaio, Monisha Samuel, Benjamin Scicluna, Bieke Soen, Anneleen Steels, Johannes V Swinnen, Maarit Takatalo, Safia Thaminy, Clotilde Théry, Joeri Tulkens, Isabel Van Audenhove, Susanne van der Grein, Alan Van Goethem, Martijn J van Herwijnen, Guillaume Van Niel, Nadine Van Roy, Alexander R Van Vliet, Niels Vandamme, Suzanne Vanhauwaert, Glenn Vergauwen, Frederik Verweij, Annelynn Wallaert, Marca Wauben, Kenneth W Witwer, Marijke I Zonneveld, Olivier De Wever, Jo Vandesompele, An Hendrix. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research. Nature Methods, 2017; 14 (3): 228 DOI: 10.1038/nmeth.4185
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Ghent University. "Cookbook recipes on extracellular vesicles." ScienceDaily. ScienceDaily, 1 March 2017. <www.sciencedaily.com/releases/2017/03/170301092657.htm>.
Ghent University. (2017, March 1). Cookbook recipes on extracellular vesicles. ScienceDaily. Retrieved May 8, 2017 from www.sciencedaily.com/releases/2017/03/170301092657.htm
Ghent University. "Cookbook recipes on extracellular vesicles." ScienceDaily. www.sciencedaily.com/releases/2017/03/170301092657.htm (accessed May 8, 2017).