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Novel mechanism that detains mobile genes in plant genome

Date:
March 10, 2017
Source:
Hokkaido University
Summary:
A team researchers has discovered a hitherto-unknown mechanism that detains transposable elements or “mobile genes” - which can move and insert into new positions in plant genomes.
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At 25C, Tam3 is immobile and Tam3 transposase are detained within the plasma membrane in a snapdragon cell (left panels). At 15C, however, the transposase is conspicuously present in the cell nucleus (right panels).
Credit: Zhou H. et al., Plant Physiology, December 22, 2016

A team of Hokkaido University researchers has discovered a hitherto-unknown mechanism that detains transposable elements or "mobile genes" -- which can move and insert into new positions in plant genomes.

Transposable elements (TEs), also known as mobile genes, are considered parasites of host genomes because they act as powerful mutagens. If not kept in check, they can cause gene disruption, genome rearrangement and genomic takeover. Thus, an essential function of organisms is controlling the movements of this troublemaker. Until now, all identified TE regulations were epigenetic-dependent, meaning that the production of TE proteins are suppressed.

A TE called Tam3 in snapdragons (Antirrhinum majus) can be regulated into active and inactive states through temperature fluctuations. It is thus possible for researchers to identify a mechanism whereby TE falls into an inactive state. The Hokkaido University research team then focused on the Tam 3 transposase protein, which is produced by the TE to enable it to move, and by employing various means investigated its positions in the cell.

According to their research, snapdragons detained Tam3 transposase within the plasma membrane when Tam3 was inactivated. When Tam3 was activated, Tam3 moved to the cell nucleus, where it is normally found.

The team also found that a structure called "Znf-BED" within Tam3 transposase plays a pivotal role in detaining Tam3 at the plasma membrane. When part of Znf-BED was changed, the transposase did not move to the plasma membrane and instead entered the cell nucleus. The team thus suggests that unknown protein produced by snapdragons binds to Tam3 transposase through Znf-BED, and detains them at the plasma membrane.

"It is the first time that such a TE detainment has been discovered," says Kaien Fujino in the research team. "The newly-found mechanism, which detains TEs after proteins are produced, is different from epigenetic regulation, where gene expression is controlled before protein is generated. Our findings should facilitate research on similar mechanisms of mobile elements in other organisms."


Story Source:

Materials provided by Hokkaido University. Note: Content may be edited for style and length.


Journal Reference:

  1. Hua Zhou, Megumi Hirata, Ryo Osawa, Kaien Fujino, Yuji Kishima. Detainment of Tam3 Transposase at Plasma Membrane by Its BED-Zinc Finger Domain. Plant Physiology, 2017; 173 (2): 1492 DOI: 10.1104/pp.16.00996

Cite This Page:

Hokkaido University. "Novel mechanism that detains mobile genes in plant genome." ScienceDaily. ScienceDaily, 10 March 2017. <www.sciencedaily.com/releases/2017/03/170310091515.htm>.
Hokkaido University. (2017, March 10). Novel mechanism that detains mobile genes in plant genome. ScienceDaily. Retrieved May 29, 2017 from www.sciencedaily.com/releases/2017/03/170310091515.htm
Hokkaido University. "Novel mechanism that detains mobile genes in plant genome." ScienceDaily. www.sciencedaily.com/releases/2017/03/170310091515.htm (accessed May 29, 2017).

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