A lightning-fast flu virus detector

Conductive polymers are a class of carbon-based molecules that conduct electricity, but can also be used in biological environments. They are very attractive materials for biosensor applications because researchers can easily attach biomolecules to the polymers, which allow them to bind with specific targets, such as flu viruses. In this study, poly(3,4-ethylenedioxythiophene) (PEDOT) was modified with a functional group that binds with the H1N1 human influenza virus, but not avian flu strains. "Conducting polymers have several advantages over inorganic counterparts," explains corresponding author Yuji Miyahara. "These include the ability to conduct both electrical and ionic carriers, mechanical flexibility, low cytotoxicity, low-cost production by casting or printing, and tunable properties via chemical synthesis or doping."

To construct the biosensor, the polymer film was placed between two electrodes. When a solution containing H1N1, which carries a tiny positive charge on its exterior shell, was added, some of the viruses stuck to the polymer and increased the voltage measured by the electrodes. This electrical method allows the sensor to detect the presence of miniscule amounts of the virus. Viral loads are often measured in hemagglutination units (HAU). The new sensor can detect viral concentrations as small as 0.013 HAU. By comparison, commercially available kits that use immunochromatographic tests only work for concentrations greater than about 1.13 HAU. This represents an almost 100-fold increase in sensitivity. Study coauthor Shoji Yamaoka stressed the clinical applications of the device. "We developed a conducting polymer-based sensor that can recognize a specific virus, which makes it a good candidate for wearable monitoring and point-of-care testing."