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Biomimetics: Artificial receptor distinguishes between male and female hormones

Study opens the door to developing ultrasensitive analytical devices for medical and sports applications

Date:
April 22, 2019
Source:
Tokyo Institute of Technology
Summary:
Researchers at Tokyo Tech have revealed that an artificial receptor preferentially binds male steroid hormones from a mixture of male and female hormones in water. Based on their findings, they succeeded in the preparation of a prototype detection system for male hormones at the nanogram level. This achievement could lead to the development of ultrasensitive analytical devices for medical diagnostics and anti-doping testing in sports.
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Chemists at Tokyo Tech's Laboratory for Chemistry and Life Science have designed and developed a capsule-shaped synthetic receptor that can distinguish between male and female steroid hormones. Namely, the receptor displays unusual high binding affinity toward androgenic[1] male hormones in water.

Published today in Science Advances, their achievement is a prime example of biomimetic design[2] -- the creation of systems that mimic ideas from nature. "Natural biological receptors can recognize tiny structural differences between male and female steroid hormones using their protein pockets," the authors say. "However, it has been challenging to emulate this function artificially until now."

The key to their breakthrough was the unique design of the cavity (mimicking the natural pocket but using unnatural components) within the receptor. This cavity, encircled by polyaromatic frameworks[3] held together with metal ions, enabled the receptor to act as a semi-rigid container -- one flexible enough to complement the shape of the hormone and to induce effective bonding interactions.

The study, conducted by Michito Yoshizawa, Masahiro Yamashina and co-workers, is a continuation of the team's previous work on developing innovative nanocapsules for a wide range of biosensing applications in the medical and environmental fields.

Their experiments showed that the synthetic receptor preferentially binds steroid sex hormones in an order similar to natural androgen receptors, beginning with male hormones such as testosterone and androsterone, followed by female hormones such as progesterone and beta-estradiol. When placed in a mixture of male and female hormones suspended in an aqueous solution at 60 degrees Celsius for ten minutes, the receptor exclusively bound testosterone with more than 98% selectivity. This high level of selectivity was achieved even when the mixture contained a large excess of female hormones.

Using X-ray crystallographic analysis, the researchers observed that the spherical cavity is distorted into an elliptical shape upon encapsulation of testosterone. They say that this conformational change contributes to the enhancement of intermolecular interactions between the receptor and the hormone.

Going one step further, the team devised a way of using the receptor to detect extremely small amounts of a male hormone. They prepared a receptor-dye complex that emits bluish green fluorescence without testosterone. By adding a nanogram amount of testosterone, the fluorescence decreased considerably upon the encapsulation, representing a remarkable new ultrasensitive detection method.

"We envision that our synthetic receptor will be used for the development of practical, ultrasensitive analytical devices for steroid sex hormones, ranging from medical tools to doping controls in sports, in the near future," the researchers say.

Technical terms

[1] Androgenic: Describing a group of steroid sex hormones predominantly involved in regulating the development of the male reproductive system.

[2] Biomimetic design: A research field that takes inspiration from biological or natural systems (such as structures and functions) for the development of novel materials and devices.

[3] Polyaromatic frameworks: Planar molecular structures composed of several benzene rings fused together.

Related links

Akita-Yoshizawa Lab. http://www.res.titech.ac.jp/~smart/smart_e.html

Michito Yoshizawa -- Novel nanostructures using self-assembly https://www.titech.ac.jp/english/research/stories/yoshizawa.html

Exact mass analysis of sulfur clusters upon encapsulation by a polyaromatic capsular matrix https://www.nature.com/articles/s41467-017-00605-5

Sweet success: Nanocapsule perfectly binds sucrose in water https://www.titech.ac.jp/english/news/2017/039190.html


Story Source:

Materials provided by Tokyo Institute of Technology. Note: Content may be edited for style and length.


Journal Reference:

  1. Masahiro Yamashina, Takahiro Tsutsui, Yoshihisa Sei, Munetaka Akita, Michito Yoshizawa. A polyaromatic receptor with high androgen affinity. Science Advances, 2019; 5 (4): eaav3179 DOI: 10.1126/sciadv.aav3179

Cite This Page:

Tokyo Institute of Technology. "Biomimetics: Artificial receptor distinguishes between male and female hormones." ScienceDaily. ScienceDaily, 22 April 2019. <www.sciencedaily.com/releases/2019/04/190422082246.htm>.
Tokyo Institute of Technology. (2019, April 22). Biomimetics: Artificial receptor distinguishes between male and female hormones. ScienceDaily. Retrieved April 18, 2024 from www.sciencedaily.com/releases/2019/04/190422082246.htm
Tokyo Institute of Technology. "Biomimetics: Artificial receptor distinguishes between male and female hormones." ScienceDaily. www.sciencedaily.com/releases/2019/04/190422082246.htm (accessed April 18, 2024).

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