New! Sign up for our free email newsletter.
Science News
from research organizations

Mutant bacterial receptor could point to new therapies against opportunistic pathogen

Date:
June 13, 2019
Source:
PLOS
Summary:
Researchers have developed a new mutant version of a receptor used by a bacterial pathogen for a chemical communication process called quorum sensing, according to a new study.
Share:
FULL STORY

Researchers have developed a new mutant version of a receptor used by a bacterial pathogen for a chemical communication process called quorum sensing, according to a study published June 13 in the open-access journal PLOS Pathogens by Bonnie Bassler of Princeton University, and colleagues. As the authors note, the mutant receptor could be used to identify therapeutic compounds that inhibit quorum sensing, fulfilling an urgent medical need.

The human pathogen Pseudomonas aeruginosa uses quorum sensing to orchestrate group behaviors, including biofilm formation and the production of infection-promoting molecules called virulence factors. Quorum sensing, which is essential for P. aeruginosa to be a pathogen, relies on the production, release, and detection of extracellular signal molecules called autoinducers. Autoinducers are bound by partner receptor proteins, and together, autoinducer-receptor complexes activate the transcription of quorum-sensing genes. In the new study, Bassler and colleagues identify, purify, and characterize a mutant version of the P. aeruginosa RhlR quorum-sensing receptor, which they call RhlR*.

Remarkably, RhlR* does not require its partner autoinducer to function. P. aeruginosa carrying RhlR* can properly form biofilms, produce virulence factors, and infect roundworms. Because RhlR* does not rely on an autoinducer to function, biochemical and genetic analyses that were previously not possible with RhlR could be performed with RhlR*. The findings provide new insight into the workings of other P. aeruginosa quorum-sensing components, most notably, the PqsE enzyme that functions together with RhlR to control virulence factor production. The authors propose that RhlR* is an especially valuable tool for learning about cell-cell communication and virulence in P. aeruginosa, a pathogen of high clinical relevance.

The authors add, "To our knowledge, this is the first report of a quorum-sensing receptor of this class that functions without its partner autoinducer molecule, opening up entirely new possibilities for study of virulence in Pseudomonas aeruginosa, a globally important pathogen."


Story Source:

Materials provided by PLOS. Note: Content may be edited for style and length.


Journal Reference:

  1. Amelia R. McCready, Jon E. Paczkowski, Jian-Ping Cong, Bonnie L. Bassler. An autoinducer-independent RhlR quorum-sensing receptor enables analysis of RhlR regulation. PLOS Pathogens, 2019; 15 (6): e1007820 DOI: 10.1371/journal.ppat.1007820

Cite This Page:

PLOS. "Mutant bacterial receptor could point to new therapies against opportunistic pathogen." ScienceDaily. ScienceDaily, 13 June 2019. <www.sciencedaily.com/releases/2019/06/190613143529.htm>.
PLOS. (2019, June 13). Mutant bacterial receptor could point to new therapies against opportunistic pathogen. ScienceDaily. Retrieved July 12, 2024 from www.sciencedaily.com/releases/2019/06/190613143529.htm
PLOS. "Mutant bacterial receptor could point to new therapies against opportunistic pathogen." ScienceDaily. www.sciencedaily.com/releases/2019/06/190613143529.htm (accessed July 12, 2024).

Explore More

from ScienceDaily

RELATED STORIES