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Wavelength-encoded laser particles for massively multiplexed cell tagging

Date:
July 23, 2019
Source:
Massachusetts General Hospital
Summary:
Researchers describe a new class of biocompatible probes, laser particles that can be inserted inside living cells.
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A new study, "Wavelength-encoded laser particles for massively multiplexed cell tagging," by scientists in the Wellman Center for Photomedicine has been published in Nature Photonics.

According to the study's researchers, "We have developed a new class of probes, laser particles, which are tiny lasers that can be inserted inside living cells. These laser particles are biocompatible, occupy only 0.1% of the volume of a typical cell and don't interfere with its normal behavior. We use the light emitted by these probes to tag and track cells as they move throughout complex biological samples. They can be thought as barcodes that can be used to distinguish cells from one another.

Conventional probes used to tag cells are based on fluorescence emission; scientists use different fluorescent molecules with different colors to distinguish different cells. Due to intrinsic limitations, however, fluorescence can only provide a handful of distinguishable colors, usually up to 4-5. Our new probes emit laser light, which can produce many more distinguishable colors, around 400 in this work.

We have used these laser particles to tag tumor cells and track their individual movement for days in a tumor spheroid (a system which mimics the growth of a tumor). In the future, we will be able to use this information to understand how tumors grow and identify specific cells with a higher potential of forming metastasis. It will be possible to single out these cells and perform further studies on them, like sequencing of their genetic profile. This will allow us to target specific genes with more intentionality, improving the options with which we can treat tumors in order to stop their spreading and formation of metastases."


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Materials provided by Massachusetts General Hospital. Note: Content may be edited for style and length.


Journal Reference:

  1. Nicola Martino, Sheldon J. J. Kwok, Andreas C. Liapis, Sarah Forward, Hoon Jang, Hwi-Min Kim, Sarah J. Wu, Jiamin Wu, Paul H. Dannenberg, Sun-Joo Jang, Yong-Hee Lee, Seok-Hyun Yun. Wavelength-encoded laser particles for massively multiplexed cell tagging. Nature Photonics, 2019; DOI: 10.1038/s41566-019-0489-0

Cite This Page:

Massachusetts General Hospital. "Wavelength-encoded laser particles for massively multiplexed cell tagging." ScienceDaily. ScienceDaily, 23 July 2019. <www.sciencedaily.com/releases/2019/07/190723142944.htm>.
Massachusetts General Hospital. (2019, July 23). Wavelength-encoded laser particles for massively multiplexed cell tagging. ScienceDaily. Retrieved December 7, 2023 from www.sciencedaily.com/releases/2019/07/190723142944.htm
Massachusetts General Hospital. "Wavelength-encoded laser particles for massively multiplexed cell tagging." ScienceDaily. www.sciencedaily.com/releases/2019/07/190723142944.htm (accessed December 7, 2023).

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