New! Sign up for our free email newsletter.
Science News
from research organizations

Getting the most therapeutic potential out of cells

Researchers find environment can boost quantity, potency of therapeutic particles

Date:
November 29, 2021
Source:
University of Illinois at Chicago
Summary:
A simple change in the way donor cells are processed can maximize a single cell's production of extracellular vesicles, which are small nanoparticles naturally secreted by cells, according to new research.
Share:
FULL STORY

A simple change in the way donor cells are processed can maximize a single cell's production of extracellular vesicles, which are small nanoparticles naturally secreted by cells, according to new research from researchers at the University of Illinois Chicago.

The finding offers new avenues for research around cellular therapies, which use transplanted cells -- like stem cells or immune cells, either from the patient or a donor -- to help the body heal or work better, and patients and their doctors want the most bang for their buck in terms of potency. For injuries in the lungs, like those caused by acute respiratory distress syndrome, treatments that use extracellular vesicles have shown promise, but remain expensive and limited by the number of donated cells needed to reach a therapeutic level.

The researchers, led by Jae-Won Shin, have been studying how extracellular vesicles work. Through experiments, they found that altering the material in which the donor cells are processed can have a strong impact on the potency of extracellular vesicles.

"We were very surprised that a simple environmental change could have such a significant impact," said Shin, UIC assistant professor in the department of pharmacology and regenerative medicine and the department of biomedical engineering. "This tells us that cells interact differently in different tissues, and this impacts how they secrete extracellular vesicles and influence other cells around them."

The key, they found, was using a soft hydrogel material that more closely resembles the natural environment of tissues to prepare the particles. When they compared the particles cultured from cells in traditional materials with those cultured in a softer material, they saw that the extracellular vesicles were secreted in a greater quantity in the softer substrate.

"In the stiff substrates, cytoskeletal structures in cells are dense and less flexible. This makes it difficult for extracellular vesicles to exit the cells. But in the soft substrate, these structures are less dense, more bendable and more spread out, making the environment more conducive to the secretion of the particles by cells," said first author Stephen Lenzini, a UIC alumnus who worked on the study in Shin's lab as a graduate student.

Shin said, "That's why fewer donor cells are needed to produce the same number of particles."

They also compared the therapeutic potential of the particles produced in the different materials. They observed that the same dose of extracellular vesicles produced from the softer substrate was much more effective at facilitating repair processes than the extracellular vesicles produced from a traditional harder substrate.

"Understanding this opens the door for many new avenues of investigation for lab and clinical trials of treatments that use donor extracellular vesicles to repair damaged tissues, like which occurs in the lungs of some COVID-19 patients who face complications like ARDS," he said.


Story Source:

Materials provided by University of Illinois at Chicago. Note: Content may be edited for style and length.


Journal Reference:

  1. Stephen Lenzini, Koushik Debnath, Jagdish C. Joshi, Sing Wan Wong, Kriti Srivastava, Xue Geng, Ik Sung Cho, Angela Song, Raymond Bargi, James C. Lee, Gary C.H. Mo, Dolly Mehta, Jae-Won Shin. Cell–Matrix Interactions Regulate Functional Extracellular Vesicle Secretion from Mesenchymal Stromal Cells. ACS Nano, 2021; 15 (11): 17439 DOI: 10.1021/acsnano.1c03231

Cite This Page:

University of Illinois at Chicago. "Getting the most therapeutic potential out of cells." ScienceDaily. ScienceDaily, 29 November 2021. <www.sciencedaily.com/releases/2021/11/211129122744.htm>.
University of Illinois at Chicago. (2021, November 29). Getting the most therapeutic potential out of cells. ScienceDaily. Retrieved July 21, 2024 from www.sciencedaily.com/releases/2021/11/211129122744.htm
University of Illinois at Chicago. "Getting the most therapeutic potential out of cells." ScienceDaily. www.sciencedaily.com/releases/2021/11/211129122744.htm (accessed July 21, 2024).

Explore More

from ScienceDaily

RELATED STORIES