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Mistaken identity cleared up of foodborne pathogen causing severe symptoms in children

Novel real-time PCR method might become diagnostic tool targeting emerging bacterium responsible for food poisoning outbreaks

Date:
May 23, 2024
Source:
Osaka Metropolitan University
Summary:
Researchers have developed a quantitative real-time PCR-based detection method for the emerging zoonotic organism Escherichia albertii. They found that E. albertii survives in the human intestinal tract for about four weeks at least.
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Novel real-time PCR method might become diagnostic tool targeting emerging bacterium responsible for food poisoning outbreaks.

The prevalence of pathogenic E. coli has meant the frequent misidentification of a similar bacterium of the Escherichia genus. E. albertii is an emerging zoonotic foodborne pathogen, first isolated in Bangladesh in 1991. Large-scale outbreaks of food poisoning caused by E. albertii have since been reported especially in Japan, causing severe symptoms in both children and adults.

In the hopes of establishing a diagnostic method, a joint research group led by Professor Shinji Yamasaki and Dr. Sharda Prasad Awasthi, a specially appointed associate professor, from the Graduate School of Veterinary Science at Osaka Metropolitan University, have developed a way to detect E. albertii more accurately using a quantitative real-time PCR method.

Specimen examination using this technique showed that E. albertii survived in the human intestinal tract for approximately four weeks and continued to be found in feces. The identical genotype of the bacterial DNA of E. albertii that infected siblings also suggested that intrafamilial transmission may have occurred.

"These results and a novel real-time PCR developed in this study are expected to contribute not only to the selection of appropriate treatment for E. albertii gastroenteritis, but also to the elucidation of the source and route of infection," Professor Yamasaki declared.


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Materials provided by Osaka Metropolitan University. Note: Content may be edited for style and length.


Journal Reference:

  1. Sharda Prasad Awasthi, Akira Nagita, Noritoshi Hatanaka, Jayedul Hassan, Bingting Xu, Atsushi Hinenoya, Shinji Yamasaki. Detection of prolong excretion of Escherichia albertii in stool specimens of a 7-year-old child by a newly developed Eacdt gene-based quantitative real-time PCR method and molecular characterization of the isolates. Heliyon, 2024; 10 (9): e30042 DOI: 10.1016/j.heliyon.2024.e30042

Cite This Page:

Osaka Metropolitan University. "Mistaken identity cleared up of foodborne pathogen causing severe symptoms in children." ScienceDaily. ScienceDaily, 23 May 2024. <www.sciencedaily.com/releases/2024/05/240523112606.htm>.
Osaka Metropolitan University. (2024, May 23). Mistaken identity cleared up of foodborne pathogen causing severe symptoms in children. ScienceDaily. Retrieved June 19, 2024 from www.sciencedaily.com/releases/2024/05/240523112606.htm
Osaka Metropolitan University. "Mistaken identity cleared up of foodborne pathogen causing severe symptoms in children." ScienceDaily. www.sciencedaily.com/releases/2024/05/240523112606.htm (accessed June 19, 2024).

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