Featured Research

from universities, journals, and other organizations

Chemist To Map DNA's Surface, Uncovering Details That Will Show How Structure Abets Function

Date:
December 2, 2004
Source:
Boston University
Summary:
In a second round of funding for technology-related research that will contribute to the international research effort known as ENCODE, the National Human Genome Research Initiative (NHGRI) is supporting a Boston University-based effort to map the topography of the DNA molecule. Prof. Thomas Tullius, chairman of Boston University’s Department of Chemistry, has received a three-year, $870,000 NHGRI grant to map the bumps, dips, and turns that characterize the surface of "naked" DNA.

Boston -- In a second round of funding for technology-related research that will contribute to the international research effort known as ENCODE, the National Human Genome Research Initiative (NHGRI) is supporting a Boston University-based effort to map the topography of the DNA molecule. Prof. Thomas Tullius, chairman of Boston University’s Department of Chemistry, has received a three-year, $870,000 NHGRI grant to map the bumps, dips, and turns that characterize the surface of "naked" DNA.

Tullius’s research will give scientists a finely detailed picture of how the most fundamental aspects of naked DNA — DNA without proteins bound to its surface — influence its function.

Tullius is one of six principal investigators to receive technology development grants from NHGRI, a part of the National Institutes of Health. The new grants, which total $5.5 million, represent the latest infusion of support to researchers selected for ENCODE projects. ENCODE, or encyclopedia of DNA elements, picks up the search for understanding the human genome where the Human Genome Project left off. Composed of scientists from government, industry, and academia from throughout the world, ENCODE is dedicated to producing a comprehensive catalog of elements crucial to biological function in the roughly 98 percent of the human genome that does not code for proteins.

ENCODE researchers are bringing their individual investigations to bear on a particular 1 percent of the genome selected by ENCODE coordinators at NHGRI. Tullius’s research on this designated genomic neighborhood aims to decipher the patterns of its landscape and, in the process, build tools that researchers can use to map protein-binding sites.

His research has three goals: to build a database of patterns of DNA’s sequence and structure found using a special probe, to develop computational methods that can predict these patterns in any sequence of DNA, and to use these experimental and computational approaches to build structural maps of the genome.

To build the database, Tullius will expand earlier research efforts to determine patterns of naked DNA using a chemical probe called a hydroxyl radical. These radicals can spot a particular hydrogen atom in a deoxyribose, a component part of DNA, and can cleave the DNA molecule at that point. The pattern of these cleavages provides a picture of the surface of DNA that is accessible to the probe.

By applying this probe to the ENCODE-selected genome segment, Tullius will gather experimental data that he will use to achieve his second aim: the development of a robust, computational model for predicting cleavage patterns in any DNA sequence.

Tullius plans to then build maps of protein-binding sites and sequences critical to the folding of chromatin, the nuclear structure that condenses to form chromosomes during DNA replication. DNA bound up as chromatin has a complex structure — it twists around histone proteins, turns upon itself, and, in general, is not an easily accessible item, especially for proteins that need to bind to it for transcription. It is known, however, that this structure is dynamic and that DNA spontaneously unwraps to allow proteins to bind. By applying both his experimental and computational techniques — chemical probes and pattern-discerning software — Tullius will be able to build three-dimensional structural maps of naked DNA, giving researchers a detailed idea not only of where regulatory proteins bind to genomic DNA, but also of how DNA’s structure abets its function.

The six 2004 technology development grants are the second set of such grants awarded by NHGRI. Grants for the first set also resulted in funding work being done by a BU researcher. In 2003, Zhiping Weng, an assistant professor of biomedical engineering in BU’s College of Engineering, received support for her work to develop or improve technologies used to determine how genetic elements, associated with what are known as promoter regions, act individually and collaboratively to regulate protein-encoding genes.

Other second-round grants went to researchers at the Genome Institute of Singapore; Intronn Inc., Gaithersburg, Md.; The Research Foundation of the State University of New York, Albany; The Salk Institute, La Jolla, Calif.; and the University of Texas, Austin.

Scientists in the Boston University’s Department of Chemistry investigate questions in theoretical chemistry, chemical physics, photochemistry, inorganic and organic chemistry, physical chemistry, and biochemistry. Boston University, with an enrollment of more than 29,000 in its 17 schools and colleges, is the fourth-largest independent university in the United States.


Story Source:

The above story is based on materials provided by Boston University. Note: Materials may be edited for content and length.


Cite This Page:

Boston University. "Chemist To Map DNA's Surface, Uncovering Details That Will Show How Structure Abets Function." ScienceDaily. ScienceDaily, 2 December 2004. <www.sciencedaily.com/releases/2004/11/041123163103.htm>.
Boston University. (2004, December 2). Chemist To Map DNA's Surface, Uncovering Details That Will Show How Structure Abets Function. ScienceDaily. Retrieved September 2, 2014 from www.sciencedaily.com/releases/2004/11/041123163103.htm
Boston University. "Chemist To Map DNA's Surface, Uncovering Details That Will Show How Structure Abets Function." ScienceDaily. www.sciencedaily.com/releases/2004/11/041123163103.htm (accessed September 2, 2014).

Share This




More Health & Medicine News

Tuesday, September 2, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Get on Your Bike! London Cycling Popularity Soars Despite Danger

Get on Your Bike! London Cycling Popularity Soars Despite Danger

AFP (Sep. 1, 2014) Wedged between buses, lorries and cars, cycling in London isn't for the faint hearted. Nevertheless the number of people choosing to bike in the British capital has doubled over the past 15 years. Duration: 02:27 Video provided by AFP
Powered by NewsLook.com
Can You Train Your Brain To Eat Healthy?

Can You Train Your Brain To Eat Healthy?

Newsy (Sep. 1, 2014) New research says if you condition yourself to eat healthy foods, eventually you'll crave them instead of junk food. Video provided by Newsy
Powered by NewsLook.com
We've Got Mites Living In Our Faces And So Do You

We've Got Mites Living In Our Faces And So Do You

Newsy (Aug. 30, 2014) A new study suggests 100 percent of adult humans (those over 18 years of age) have Demodex mites living in their faces. Video provided by Newsy
Powered by NewsLook.com
Liberia Continues Fight Against Ebola

Liberia Continues Fight Against Ebola

AFP (Aug. 30, 2014) Authorities in Liberia try to stem the spread of the Ebola epidemic by raising awareness and setting up sanitation units for people to wash their hands. Duration: 00:41 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins