Featured Research

from universities, journals, and other organizations

PCRM Develops New Animal-free Insulin Assay

Date:
February 15, 2005
Source:
Physicians Committee For Responsible Medicine
Summary:
If you're an organization dedicated to humane alternatives to the use of animals in research and you want to conduct research of your own that requires using animals as part of the testing, what do you do? In the case of the Physicians Committee for Responsible Medicine, you invent your own test.

WASHINGTON (February 9, 2005) -- If you're an organization dedicated to humane alternatives to the use of animals in research and you want to conduct research of your own that requires using animals as part of the testing, what do you do? In the case of the Physicians Committee for Responsible Medicine, you invent your own test.

Related Articles


PCRM president Neal Barnard, M.D., announced today that PCRM has developed what it calls the world's first 'cruelty-free' insulin assay, a test used to measure insulin levels in individuals with diabetes. The assay, which uses no animals, was developed as part of PCRM's ongoing clinical trials to test the effects of a low-fat, vegan diet on patients with type 2 diabetes.

"We only had two options available to us when we began our diabetes trials," said Dr. Barnard. "One, we could use test kits with insulin antibodies grown in vivo--literally from cells injected into the abdomens of live mice--or we could use kits containing antibodies produced from cells cultured with fetal calf serum. Neither was acceptable to us."

The answer? Develop an in-vitro, or test-tube, procedure using a synthetic replacement for the fetal calf serum used as a culturing medium in millions of medical tests every year.

After months of painstaking detective work, PCRM research analyst Megha Even, M.S., working with BiosPacific, an Emoryville, California, lab, succeeded in culturing cells using an animal component-free, peptide- and protein-free, media supplement as a replacement for calf serum--basically a synthetic formula with cofactors and trace elements that promote cell growth. Then, in collaboration with Linco Research of St. Charles, Missouri, Even successfully incorporated antibodies grown in the medium into a test kit for human insulin.

A report on the new methodology will be published soon in a peer-reviewed journal in conjunction with Linco. Even will present her findings at the "Experimental Biology 2005" scientific conference in San Diego, April 2-6. The new assay kits are available commercially from Linco.

"We hope that by making the test readily available and competitively priced, researchers and medical labs will use it," said Barnard. "We have proven that if researchers are willing to make the effort, there are effective, humane alternatives to animal-based assays and other testing procedures--alternatives that could help save the lives of millions of people and animals."

There are an estimated 194 million people worldwide with diabetes. More than 15 million Americans suffer from the disease and resulting complications.

###

Founded in 1985, the Physicians Committee for Responsible Medicine is a nonprofit health organization that promotes preventive medicine, conducts clinical research studies, opposes unethical human experimentation, and promotes alternatives to animal research.


Story Source:

The above story is based on materials provided by Physicians Committee For Responsible Medicine. Note: Materials may be edited for content and length.


Cite This Page:

Physicians Committee For Responsible Medicine. "PCRM Develops New Animal-free Insulin Assay." ScienceDaily. ScienceDaily, 15 February 2005. <www.sciencedaily.com/releases/2005/02/050212192617.htm>.
Physicians Committee For Responsible Medicine. (2005, February 15). PCRM Develops New Animal-free Insulin Assay. ScienceDaily. Retrieved December 20, 2014 from www.sciencedaily.com/releases/2005/02/050212192617.htm
Physicians Committee For Responsible Medicine. "PCRM Develops New Animal-free Insulin Assay." ScienceDaily. www.sciencedaily.com/releases/2005/02/050212192617.htm (accessed December 20, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Saturday, December 20, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Researchers Test Colombian Village With High Alzheimer's Rates

Researchers Test Colombian Village With High Alzheimer's Rates

AFP (Dec. 19, 2014) In Yarumal, a village in N. Colombia, Alzheimer's has ravaged a disproportionately large number of families. A genetic "curse" that may pave the way for research on how to treat the disease that claims a new victim every four seconds. Duration: 02:42 Video provided by AFP
Powered by NewsLook.com
Monarch Butterflies Descend Upon Mexican Forest During Annual Migration

Monarch Butterflies Descend Upon Mexican Forest During Annual Migration

Reuters - Light News Video Online (Dec. 19, 2014) Millions of monarch butterflies begin to descend onto Mexico as part of their annual migration south. Rough Cut (no reporter narration) Video provided by Reuters
Powered by NewsLook.com
The Best Protein-Filled Foods to Energize You for the New Year

The Best Protein-Filled Foods to Energize You for the New Year

Buzz60 (Dec. 19, 2014) The new year is coming and nothing will energize you more for 2015 than protein-filled foods. Fitness and nutrition expert John Basedow (@JohnBasedow) gives his favorite high protein foods that will help you build muscle, lose fat and have endless energy. Video provided by Buzz60
Powered by NewsLook.com
Birds Might Be Better Meteorologists Than Us

Birds Might Be Better Meteorologists Than Us

Newsy (Dec. 19, 2014) A new study suggests a certain type of bird was able to sense a tornado outbreak that moved through the U.S. a day before it hit. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins