Featured Research

from universities, journals, and other organizations

DNA With Three Base Pairs - A Step Towards Expanding The Genetic Code

Date:
April 7, 2005
Source:
American Chemical Society
Summary:
Scientists at The Scripps Research Institute in La Jolla, California are reporting at the 229th national meeting of the American Chemical Society progress toward the creation of a system for replicating a modified form of DNA containing an unnatural base pair.

Scientists at The Scripps Research Institute in La Jolla, California are reporting at the 229th national meeting of the American Chemical Society progress toward the creation of a system for replicating a modified form of DNA containing an unnatural base pair.

According to the Scripps Research scientists, this finding is a significant step towards expanding the genetic code and the ability of DNA to act as an information storage and retrieval system in the test tube and in simple, engineered organisms, such as yeast or bacteria. DNA with three or more base pairs could find broad applications in a number of fields, including biotechnology, medicine, data storage, and security.

Instead of just the canonical base pairs “G-C“ or guanine–cytosine, and “A-T” or adenine–thymine, the Scripps Research scientists’ DNA has a third pairing: “3FB-3FB” between two unnatural bases called 3-fluorobenzene (or 3FB). Unlike other unnatural base pairs, DNA polymerases are able to replicate this base pair, albeit with reduced fidelity. To improve replication, the scientists also reported the development of a system capable of evolving polymerases to better recognize 3FB in DNA. Using a selection system some liken to evolution in the test tube, they are creating their own “polymerase” enzyme able to replicate the unnatural DNA.

While the polymerase does not replicate the unnatural DNA with the same fidelity observed in nature, (roughly one mistake for every 10 million bases of DNA copied), its fidelity is reasonable (typically making only one mistake for every1000 base pairs). This is the first time anyone has been able to replicate unnatural DNA with fidelity against every possible mispair.

“We definitely are still working on improvements, especially in fidelity,” says Scripps Research Assistant Professor Floyd Romesberg, who led the research. “Nevertheless, we are now able to replicate unnatural DNA.”

The American Chemical Society, the world’s largest scientific society, is holding its national meeting in San Diego, CA, from March 13 to 17. The talk on this research, entitled “Efforts to Expand the Genetic Code,” will be presented at 3:15 PM, Monday, March 14 during the Biomimetic Polymers symposium (POLY 193) at the San Diego Marriott in the Manchester rooms 1-2.


Story Source:

The above story is based on materials provided by American Chemical Society. Note: Materials may be edited for content and length.


Cite This Page:

American Chemical Society. "DNA With Three Base Pairs - A Step Towards Expanding The Genetic Code." ScienceDaily. ScienceDaily, 7 April 2005. <www.sciencedaily.com/releases/2005/03/050328145139.htm>.
American Chemical Society. (2005, April 7). DNA With Three Base Pairs - A Step Towards Expanding The Genetic Code. ScienceDaily. Retrieved August 2, 2014 from www.sciencedaily.com/releases/2005/03/050328145139.htm
American Chemical Society. "DNA With Three Base Pairs - A Step Towards Expanding The Genetic Code." ScienceDaily. www.sciencedaily.com/releases/2005/03/050328145139.htm (accessed August 2, 2014).

Share This




More Plants & Animals News

Saturday, August 2, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Pyrenees Orphan Bear Cub Gets Brand New Home

Pyrenees Orphan Bear Cub Gets Brand New Home

AFP (Aug. 1, 2014) The discovery of a bear cub in the Pyrenees mountains made headlines in April 2014. Despire several attempts to find the animal's mother, the cub remained alone. Now, the Pyrenees Conservation Foundation has constructed an enclosure. Duration: 00:31 Video provided by AFP
Powered by NewsLook.com
Ebola Vaccine Might Be Coming, But Where's It Been?

Ebola Vaccine Might Be Coming, But Where's It Been?

Newsy (Aug. 1, 2014) Health officials are working to fast-track a vaccine — the West-African Ebola outbreak has killed more than 700. But why didn't we already have one? Video provided by Newsy
Powered by NewsLook.com
Study Links Certain Birth Control Pills To Breast Cancer

Study Links Certain Birth Control Pills To Breast Cancer

Newsy (Aug. 1, 2014) Previous studies have made the link between birth control and breast cancer, but the latest makes the link to high-estrogen oral contraceptives. Video provided by Newsy
Powered by NewsLook.com
Rare Whale Fossil Pulled from Calif. Backyard

Rare Whale Fossil Pulled from Calif. Backyard

AP (Aug. 1, 2014) A rare whale fossil has been pulled from a Southern California backyard. The 16- to 17-million-year-old baleen whale fossil is one of about 20 baleen whale fossils known to exist. (Aug. 1) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins