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'Stubborn' Microbes Propagated WIth New Incubation Method

Date:
February 25, 2008
Source:
Northeastern University
Summary:
Researchers have discovered a way to domesticate "stubborn" microbial species. The revolutionary method capitalizes on an earlier technology to grow microorganisms in diffusion chambers incubated in natural environment. Now they have shown that a series of such incubations lead to the appearance of variants capable of growth on standard media. Additionally, they designed a "chip" allowing massively parallel growth and isolation of pure microbial cultures.

Diffusion chamber "chip" created by Northeastern University professors Slava Epstein and Kim Lewis.
Credit: Image courtesy of Northeastern University

Two Northeastern University researchers have discovered a way to domesticate “stubborn” microbial species. The revolutionary method capitalizes on their earlier technology to grow microorganisms in diffusion chambers incubated in natural environment.

Now Professors Slava Epstein and Kim Lewis, Director of Northeastern’s Antimicrobial Discovery Center, have shown that a series of such incubations lead to the appearance of variants capable of growth on standard media. Additionally, they designed a “chip” allowing massively parallel growth and isolation of pure microbial cultures.

“This new process will allow Northeastern researchers to grow microorganisms without having to painstakingly separate the strains as previous techniques required,” said Epstein, Associate Professor of Microbiology at Northeastern University.

The net effect of the new developments is a simple, high throughput technology to access a novel source of biological diversity: previously uncultivated microbial species. Microbial cultivation is a fundamental tool in both basic and applied microbiology, and invaluable in obtaining sufficient microbial biomass to harvest useful molecules it produces.

The new technology will enable growing and utilizing novel species important for application in bioremediation, alternative fuel research, and drug discovery.


Story Source:

The above story is based on materials provided by Northeastern University. Note: Materials may be edited for content and length.


Cite This Page:

Northeastern University. "'Stubborn' Microbes Propagated WIth New Incubation Method." ScienceDaily. ScienceDaily, 25 February 2008. <www.sciencedaily.com/releases/2008/02/080223121818.htm>.
Northeastern University. (2008, February 25). 'Stubborn' Microbes Propagated WIth New Incubation Method. ScienceDaily. Retrieved April 25, 2014 from www.sciencedaily.com/releases/2008/02/080223121818.htm
Northeastern University. "'Stubborn' Microbes Propagated WIth New Incubation Method." ScienceDaily. www.sciencedaily.com/releases/2008/02/080223121818.htm (accessed April 25, 2014).

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