Featured Research

from universities, journals, and other organizations

Whole Genome Sequencing Approach For Mutation Discovery

Date:
May 6, 2009
Source:
Stowers Institute for Medical Research
Summary:
Scientists have developed a "whole-genome sequencing approach" to mapping mutations in fruit flies. The novel methodology promises to reduce the time and effort required to identify mutations of biological interest.

The Stowers Institute’s Hawley Lab and Molecular Biology Facility have developed a “whole-genome sequencing approach” to mapping mutations in fruit flies. The novel methodology promises to reduce the time and effort required to identify mutations of biological interest.

The team mapped a fruit-fly mutation caused by the compound ethyl methanesulfonate (EMS) by determining the DNA sequence of the mutant fly’s genome. The results provide insight into the mechanism of EMS mutageneseis and into gene conversion events involving balancer chromosomes — genetic tools used to prevent genetic recombination between homologous chromosomes during meiosis.

Model organisms like fruit flies are used in research for studying both normal biological processes and human disease. Fruit fly genes can be inserted, deleted or modified, and large numbers of flies can be randomly mutated to generate interesting phenotypes relevant to human disease. Finding the mutated gene responsible for an interesting phenotype is labor intensive and time consuming, and many mutations that cause medically relevant phenotypes are not discovered. The new approach lowers the barrier to finding mutations and greatly accelerates the discovery of genes important for human health.

“This approach will change the way fruit fly genetics is done,” said Scott Hawley, Ph.D., Investigator and co-equal senior author on the publication. “Traditional mapping approaches to identify mutations are inefficient procedures. Our whole-genome sequencing approach is fast and cost effective. Among other potential uses, it also carries the potential to pinpoint inheritable molecular characteristics that are controlled by several genes at once.”

“The traditional mapping method could take months to years depending on the complexity of the phenotype,” said Karen Staehling-Hampton, Ph.D., Managing Director of Molecular Biology and co-equal senior author on the paper. “This advance will allow us to map mutations of interest in just a few weeks. The next-generation sequencing technology used for this project is extremely exciting. It will allow researchers to sequence genomes for a few thousand dollars, a cost unheard of just a few years ago. It will also enable them to take their science in new directions and answer new questions that were not possible with traditional sequencing technology.”

Additional contributing authors from the Stowers Institute include first author Justin Blumenstiel, Ph.D., formerly a Postdoctoral Research Fellow; Aaron Noll, Bioinformatics Programmer Analyst III; Jennifer Griffiths, Research Technician III; Anoja Perera, Laboratory Manager II; Kendra Walton, Research Technician III; and William Gilliland, Ph.D., Senior Research Associate.


Story Source:

The above story is based on materials provided by Stowers Institute for Medical Research. Note: Materials may be edited for content and length.


Journal Reference:

  1. Blumenstiel et al. Identification of EMS-Induced Mutations in Drosophila melanogaster by Whole-Genome Sequencing. Genetics, 2009; 182 (1): 25 DOI: 10.1534/genetics.109.101998

Cite This Page:

Stowers Institute for Medical Research. "Whole Genome Sequencing Approach For Mutation Discovery." ScienceDaily. ScienceDaily, 6 May 2009. <www.sciencedaily.com/releases/2009/05/090505124754.htm>.
Stowers Institute for Medical Research. (2009, May 6). Whole Genome Sequencing Approach For Mutation Discovery. ScienceDaily. Retrieved April 17, 2014 from www.sciencedaily.com/releases/2009/05/090505124754.htm
Stowers Institute for Medical Research. "Whole Genome Sequencing Approach For Mutation Discovery." ScienceDaily. www.sciencedaily.com/releases/2009/05/090505124754.htm (accessed April 17, 2014).

Share This



More Plants & Animals News

Thursday, April 17, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

The Great British Farmland Boom

The Great British Farmland Boom

Reuters - Business Video Online (Apr. 17, 2014) Britain's troubled Co-operative Group is preparing to cash in on nearly 18,000 acres of farmland in one of the biggest UK land sales in decades. As Ivor Bennett reports, the market timing couldn't be better, with farmland prices soaring over 270 percent in the last 10 years. Video provided by Reuters
Powered by NewsLook.com
Change of Diet Helps Crocodile Business

Change of Diet Helps Crocodile Business

Reuters - Business Video Online (Apr. 16, 2014) Crocodile farming has been a challenge in Zimbabwe in recent years do the economic collapse and the financial crisis. But as Ciara Sutton reports one of Europe's biggest suppliers of skins to the luxury market has come up with an unusual survival strategy - vegetarian food. Video provided by Reuters
Powered by NewsLook.com
Could Even Casual Marijuana Use Alter Your Brain?

Could Even Casual Marijuana Use Alter Your Brain?

Newsy (Apr. 16, 2014) A new study conducted by researchers at Northwestern and Harvard suggests even casual marijuana use can alter your brain. Video provided by Newsy
Powered by NewsLook.com
Thousands Of Vials Of SARS Virus Go Missing

Thousands Of Vials Of SARS Virus Go Missing

Newsy (Apr. 16, 2014) A research institute in Paris somehow misplaced more than 2,000 vials of the deadly SARS virus. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins