Featured Research

from universities, journals, and other organizations

New Accurate Diagnostic Test For Swine H1N1 Influenza Using RT-PCR Technology

Date:
June 12, 2009
Source:
Mary Ann Liebert, Inc./Genetic Engineering News
Summary:
A new, easy-to-perform method for detecting seasonal influenza A virus and the emerging H1N1 swine-derived influenza A virus in human clinical samples offers a fast, sensitive, and cost-effective diagnostic test that runs on standard laboratory equipment.

A new, easy-to-perform method for detecting both seasonal influenza A virus and the emerging H1N1 swine-derived influenza A virus in human clinical samples offers a fast, sensitive, and cost-effective diagnostic test that runs on standard laboratory equipment. This timely and broadly applicable molecular technique is described in a new article.

The recent emergence and global spread of a new swine flu virus highlights the urgent need for a reliable diagnostic test that can discriminate the H1N1 influenza virus from other strains and can be readily implemented in clinical testing laboratories. The molecular strategy described in the article in Vector-Borne and Zoonotic Diseases is based on proven and widely used Real-Time, Polymerase Chain Reaction (RT-PCR) technology.

The authors of the report describe the development of a new molecular probe that improves on the existing PCR assay used to diagnose seasonal influenza and enables detection of both the seasonal and H1N1 influenza A viruses in the same patient sample using a simple test protocol. Laetitia Ninove and colleagues from Université de la Méditerranée and Institut de Recherche pour le Développement (Marseille, France), Hôpitaux de Marseille, CEH Oxford (UK), and EHESP School of Public Health (Rennes, France) provide data to support the sensitivity and effectiveness of the SYBR Green RT-PCR one-step assay used for screening clinical samples to detect the presence of influenza A virus. In positive samples this is followed by the addition of two probes that are able to discriminate between the seasonal and swine H1N1 viruses to yield a definitive diagnosis.

Early, accurate identification of infected individuals will expedite appropriate antiviral therapy and enhance control and containment efforts. Furthermore, this new molecular test specifically amplifies and characterizes the viral genetic material, enabling rapid detection of new viral strains as they evolve. Using these genetic sequence data and making minor alterations to the PCR primers used in the assay, the test could be easily modified to detect newly emerging viral variants, including avian influenza strains.

"Early recognition of new influenza strains is vitally important for implementing effective control measures to limit spread. This cost-effective, comprehensive, and rapid test is a highly significant contribution to diagnostics that will greatly enhance our capacity to deal with future influenza outbreaks," says Stephen Higgs BSc, PhD, FRES, Editor-in-Chief of Vector-Borne and Zoonotic Diseases, and Associate Professor, Department of Pathology, Center for Biodefense & Emerging Infectious Diseases, Sealy Center for Vaccine Development and WHO Collaborating Center for Tropical Diseases, University of Texas Medical Branch, Galveston.


Story Source:

The above story is based on materials provided by Mary Ann Liebert, Inc./Genetic Engineering News. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ninove et al. A Simple Method for Molecular Detection of Swine-Origin and Human-Origin Influenza A Virus. Vector-Borne and Zoonotic Diseases, 2009; 090608115925050 DOI: 10.1089/vbz.2009.0110

Cite This Page:

Mary Ann Liebert, Inc./Genetic Engineering News. "New Accurate Diagnostic Test For Swine H1N1 Influenza Using RT-PCR Technology." ScienceDaily. ScienceDaily, 12 June 2009. <www.sciencedaily.com/releases/2009/06/090609122224.htm>.
Mary Ann Liebert, Inc./Genetic Engineering News. (2009, June 12). New Accurate Diagnostic Test For Swine H1N1 Influenza Using RT-PCR Technology. ScienceDaily. Retrieved September 23, 2014 from www.sciencedaily.com/releases/2009/06/090609122224.htm
Mary Ann Liebert, Inc./Genetic Engineering News. "New Accurate Diagnostic Test For Swine H1N1 Influenza Using RT-PCR Technology." ScienceDaily. www.sciencedaily.com/releases/2009/06/090609122224.htm (accessed September 23, 2014).

Share This



More Health & Medicine News

Tuesday, September 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Liberia Pleads for Help to Fight Ebola

Liberia Pleads for Help to Fight Ebola

AP (Sep. 22, 2014) — Liberia's finance minister is urging the international community to quickly follow through on pledges of cash to battle Ebola. Bodies are piling up in the capital Monrovia as the nation awaits more help. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Ebola Doctor Says Border Controls Critical

Ebola Doctor Says Border Controls Critical

AP (Sep. 22, 2014) — A Florida doctor who helped fight the expanding Ebola outbreak in West Africa says the disease can be stopped, but only if nations quickly step up their response and make border control a priority. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Global Ebola Aid Increasing But Critics Say It's Late

Global Ebola Aid Increasing But Critics Say It's Late

Newsy (Sep. 21, 2014) — More than 100 tons of medical supplies were sent to West Africa on Saturday, but aid workers say the global response is still sluggish. Video provided by Newsy
Powered by NewsLook.com
Sierra Leone in Lockdown to Control Ebola

Sierra Leone in Lockdown to Control Ebola

AP (Sep. 21, 2014) — Sierra Leone residents remained in lockdown on Saturday as part of a massive effort to confine millions of people to their homes in a bid to stem the biggest Ebola outbreak in history. (Sept. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins