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Characteristics Of Clarithromycin-resistant Helicobacter Pylori

July 16, 2009
World Journal of Gastroenterology
Clarithromycin resistance is an uncommon occurrence among Malaysian isolates of Helicobacter pylori strains, and the mutations A2142G and A2143G detected were associated with low-level resistance.

Clarithromycin is currently one of the antibiotics used for eradication of Helicobacter pylori. However, reports of H. pylori resistance to this antibiotic are increasing worldwide. Clarithromycin resistance has been attributed to the presence of mutations in the 23S rRNA gene, a component of the ribosome that is the protein manufacturing machinery of all living cells. There is little information on the prevalence and characteristics of clarithromycin resistance in H. pylori strains isolated from Malaysian patients.

In a research article in the World Journal of Gastroenterology, the authors determined the prevalence of resistance and characterized the types of mutations present in their resistant strains. In this study conducted by Norazah et al on strains isolated in Malaysia, a low prevalence of clarithromycin-resistant strains was noted. Transitions of adenine to guanine at positions 2142 and 2143 of the 23S rRNA of H. pylori were the main mutations found in clarithromycin-resistant isolates.

The A2142G and 2143G mutations of 23S rRNA genes in these strains can be detected easily by restriction fragment length polymorphism (RFLP) analysis of the PCR product of the 23S rRNA genes, using MboII or BsaI restriction enzymes. This eliminates the need to detect these mutations by sequencing. The result of the study demonstrates that clarithromycin resistance is present among local strains.

However, even though it is an uncommon occurrence, the possibility of the patient being infected with a resistant strain should be considered if the patient does not respond to treatment with clarithromycin, so that an alternative treatment can be given. The ability to detect clarithromycin resistance by molecular methods such as RFLP showed that this rapid method has the potential to be used as a diagnostic tool.

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The above post is reprinted from materials provided by World Journal of Gastroenterology. Note: Materials may be edited for content and length.

Journal Reference:

  1. Ahmad et al. Characterization of clarithromycin resistance in Malaysian isolates of Helicobacter pylori. World Journal of Gastroenterology, 2009; 15 (25): 3161 DOI: 10.3748/wjg.15.3161

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World Journal of Gastroenterology. "Characteristics Of Clarithromycin-resistant Helicobacter Pylori." ScienceDaily. ScienceDaily, 16 July 2009. <>.
World Journal of Gastroenterology. (2009, July 16). Characteristics Of Clarithromycin-resistant Helicobacter Pylori. ScienceDaily. Retrieved October 13, 2015 from
World Journal of Gastroenterology. "Characteristics Of Clarithromycin-resistant Helicobacter Pylori." ScienceDaily. (accessed October 13, 2015).

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