Featured Research

from universities, journals, and other organizations

New Research Supports Model For Nuclear Pore Complex

Date:
September 3, 2009
Source:
Rockefeller University
Summary:
To protect their DNA, cells in higher organisms are very choosy about what they allow in and out of their nuclei, where the genes reside. Guarding access is the job of transport machines called nuclear pore complexes, which stud the nuclear membrane. Despite these gatekeepers' conspicuously large size (they are made of 30 different proteins), they have proved largely inscrutable to researchers over the years. But bit by bit, scientists are learning how these machines work.

Paring the pore. A new model of how the nuclear pore complex might work suggests that an interaction between two proteins -- key elements of the yellow ring pictured above -- link together, forming a flexible fence around the pore's opening.
Credit: Joseph Alexander, Erik Debler and Andre Hoelz

To protect their DNA, cells in higher organisms are very choosy about what they allow in and out of their nuclei, where the genes reside. Guarding access is the job of transport machines called nuclear pore complexes, which stud the nuclear membrane. Despite these gatekeepers’ conspicuously large size (they are made of 30 different proteins), they have proved largely inscrutable to researchers over the years. But bit by bit, scientists are learning how these machines work.

Related Articles


Now a new study reveals the structure of one of the proteins that makes up this molecule-trafficking complex. Researchers have also shown how that protein interacts with a partner, supporting a model that calls for a flexible “ring” around the opening of each pore. The work could offer a key insight into an important design feature of this little-understood and evolutionarily ancient structure, an innovation fundamental to the development of nearly all multicellular life on Earth.

The research, performed by Hyuk-Soo Seo, a postdoctoral associate, and Andrι Hoelz, a research associate, both in Rockefeller University’s Laboratory of Cell Biology, determined the molecular structure of the only remaining unsolved protein in an important piece of the nuclear pore called the Nup84 complex. Nup84 is a Y-shaped element that was recently imaged in three dimensions by Martin Kampmann, also a member of the lab headed by Howard Hughes Medical Institute Investigator Gόnter Blobel.

In experiments recently published online in the Proceedings of the National Academy of Sciences, Seo, Hoelz and colleagues focused on the behavior of this newly solved protein — or nucleoporin — called Nup120, one of seven comprising the Nup84 complex. They determined that one end of Nup120, the N-terminal domain, is attached by a stretchable tether to one other protein in the complex, Nup133. Furthermore, the researchers showed in living cells that mutations to a critical region of the tether interfered with the export of messenger RNA, one of the nuclear pore’s chief responsibilities, confirming the functional importance of this loose linkage between the two proteins.

“It’s a very nice correlation from the structure to the function,” Hoelz says. “It’s the first example where we can really pin down how the [Nup84] complexes arrange with each other, and what we believe we see is a flexible ring that could expand and contract to import and export large molecules.”


Story Source:

The above story is based on materials provided by Rockefeller University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Seo et al. Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex. Proceedings of the National Academy of Sciences, 2009; 106 (34): 14281 DOI: 10.1073/pnas.0907453106

Cite This Page:

Rockefeller University. "New Research Supports Model For Nuclear Pore Complex." ScienceDaily. ScienceDaily, 3 September 2009. <www.sciencedaily.com/releases/2009/08/090829092830.htm>.
Rockefeller University. (2009, September 3). New Research Supports Model For Nuclear Pore Complex. ScienceDaily. Retrieved December 19, 2014 from www.sciencedaily.com/releases/2009/08/090829092830.htm
Rockefeller University. "New Research Supports Model For Nuclear Pore Complex." ScienceDaily. www.sciencedaily.com/releases/2009/08/090829092830.htm (accessed December 19, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, December 19, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Navy Unveils Robot Fish

Navy Unveils Robot Fish

Reuters - Light News Video Online (Dec. 18, 2014) — The U.S. Navy unveils an underwater device that mimics the movement of a fish. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com
Kids Die While Under Protective Services

Kids Die While Under Protective Services

AP (Dec. 18, 2014) — As part of a six-month investigation of child maltreatment deaths, the AP found that hundreds of deaths from horrific abuse and neglect could have been prevented. AP's Haven Daley reports. (Dec. 18) Video provided by AP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) — Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
The Hottest Food Trends for 2015

The Hottest Food Trends for 2015

Buzz60 (Dec. 17, 2014) — Urbanspoon predicts whicg food trends will dominate the culinary scene in 2015. Mara Montalbano (@maramontalbano) has the story. Video provided by Buzz60
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins