Plasmids, which are DNA molecules capable of independent replication in cells, have played an important role in gene technology. Researchers from Uppsala University in Sweden have now demonstrated that plasmid-based methods, which had been limited to single-cell organisms such as bacteria and yeasts, can be extended to mosses, opening the door to applications of a number of powerful techniques in plant research.
The findings have been published in the distinguished journal Proceedings of the National Academy of Sciences of the USA (PNAS).
Professor Hans Ronne's research team at the Department of Medical Biochemistry and Microbiology at Uppsala University works with the diminutive Physcomitrella moss, which is widely used in research because its genes are easier to "knock out" than those of other plants. Previous work has also shown that DNA introduced into Physcomitrella cells is capable of self-replication through an as yet uncharacterised process.
The new study, which was led by Dr. Eva Murιn and Ph.D. student Anders Nilsson, shows that plasmids introduced into moss cells can be rescued back to bacteria without affecting the plasmids' original structures, provided that certain conditions are met. Up to now, various kinds of rearrangements have sharply limited the use of plasmids in animal- and plant-cell research.
"Our work with plasmids in moss suggests that it will be possible to use powerful methods such as gene cloning by complementation and overexpression directly in plant cells without recourse to single-cell organisms like bacteria or yeasts," says Professor Hans Ronne. "This, in turn, may simplify basic and applied research and biotechnology involving plants."
- Muren et al. Rescue and characterization of episomally replicating DNA from the moss Physcomitrella. Proceedings of the National Academy of Sciences, 2009; DOI: 10.1073/pnas.0908037106
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