Featured Research

from universities, journals, and other organizations

Diffusion of a soluble protein through a sensory cilium

Date:
March 1, 2010
Source:
Rockefeller University Press
Summary:
Scientists have, for the first time, measured the diffusion coefficient of a protein in a primary cilium and in other major compartments of a highly polarized cell.

Calvert et al. measured protein diffusion through a sensory cilium.
Credit: Calvert, P.D. 2010. J. Gen. Physiol. doi:10.1085/jgp.200910322.

A team of researchers led by Peter Calvert (SUNY Upstate Medical University) has, for the first time, measured the diffusion coefficient of a protein in a primary cilium and in other major compartments of a highly polarized cell. The study appears in the March issue of the Journal of General Physiology.

Related Articles


Transport of proteins to and from cilia is crucial for normal cell function and survival, and interruption of transport has been implicated in degenerative diseases and neoplastic diseases, such as cancer. Researchers believe that cilia impose selective barriers to the movement of proteins, but because of the narrow and complex structure of cilia -- with diameters near or below the resolution of light microscopy -- this hypothesis has been difficult to examine.

Using confocal and multiphoton microscopy, Calvert and his team -- including William Schiesser (Lehigh University) and Edward Pugh (University of California, Davis) -- measured the mobility of PAGFP (photoactivatable green fluorescent protein) in the connecting cilium (CC) of retinal rod photoreceptors in frogs, as well as in the subcellular compartments bridged by the CC. In addition, the team measured the overall time for the protein concentration to equilibrate within and between compartments.

The results establish that the CC does not pose a major barrier to protein diffusion within the rod cell, but that the axial diffusion in each of the rod's compartments is substantially delayed relative to that in aqueous solution.

Calvert, P.D. 2010. J. Gen. Physiol. doi:10.1085/jgp.200910322.


Story Source:

The above story is based on materials provided by Rockefeller University Press. Note: Materials may be edited for content and length.


Cite This Page:

Rockefeller University Press. "Diffusion of a soluble protein through a sensory cilium." ScienceDaily. ScienceDaily, 1 March 2010. <www.sciencedaily.com/releases/2010/02/100222094748.htm>.
Rockefeller University Press. (2010, March 1). Diffusion of a soluble protein through a sensory cilium. ScienceDaily. Retrieved January 30, 2015 from www.sciencedaily.com/releases/2010/02/100222094748.htm
Rockefeller University Press. "Diffusion of a soluble protein through a sensory cilium." ScienceDaily. www.sciencedaily.com/releases/2010/02/100222094748.htm (accessed January 30, 2015).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, January 30, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Brawling Pandas Are Violently Adorable

Brawling Pandas Are Violently Adorable

Buzz60 (Jan. 29, 2015) Video of pandas play fighting at the Chengdu Research Base in China will make your day. Mara Montalbano (@maramontalbano) shows us. Video provided by Buzz60
Powered by NewsLook.com
Why Researchers Say We Should Cut Back On Biofuels

Why Researchers Say We Should Cut Back On Biofuels

Newsy (Jan. 29, 2015) Biofuels aren&apos;t the best alternative to fossil fuels, according to a new report. In fact, they&apos;re quite a bad one. Video provided by Newsy
Powered by NewsLook.com
3-D Printed Wheelchair Helps Two-Legged Dog Learn to Run

3-D Printed Wheelchair Helps Two-Legged Dog Learn to Run

Buzz60 (Jan. 29, 2015) 3-D printing helps another two-legged dog run around with his four-legged friends. Jen Markham (@jenmarkham) has the adorable video. Video provided by Buzz60
Powered by NewsLook.com
Dogs Bring on So Many Different Emotions in Their Human Best Friends

Dogs Bring on So Many Different Emotions in Their Human Best Friends

RightThisMinute (Jan. 28, 2015) From new-puppy happy tears to helpful-grocery-carrying-dog laughter, our four-legged best friends can make us feel the entire spectrum of emotions. Video provided by RightThisMinute
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins