Featured Research

from universities, journals, and other organizations

Microfluidic device rapidly orients hundreds of embryos for high-throughput experiments

Date:
December 29, 2010
Source:
Georgia Institute of Technology Research
Summary:
Researchers have developed a microfluidic device that automatically orients hundreds of fruit fly embryos to prepare them for research. The device could facilitate the study of such issues as how organisms develop their complex structures from single cells.

These are Drosophila embryos in dorsoventral cross-sectional views stained for a variety of morphogens. This view of embryos would have been difficult to obtain without the use of the microfluidic device.
Credit: Georgia Tech/Hang Lu

Researchers have developed a microfluidic device that automatically orients hundreds of fruit fly and other embryos to prepare them for research. The device could facilitate the study of such issues as how organisms develop their complex structures from single cells -- one of the most fascinating aspects of biology.

Related Articles


Scientists know that among an embryo's first major developments is the establishment of its dorsoventral axis, which runs from its back to its belly. Determining how this axis development unfolds -- specifically the presence and location of proteins during the process -- requires the ability to simultaneously monitor large numbers of embryos with different genetic backgrounds at several time points.

"Collecting and analyzing the signaling and transcriptional patterns of the dorsoventral axis typically requires manual manipulation of individual embryos to stand them on their ends, making it difficult to conduct high-throughput experiments that can achieve statistically significant results," said Hang Lu, an associate professor in the Georgia Tech School of Chemical & Biomolecular Engineering.

To enable large-scale quantitative analyses of protein positional information along the dorsoventral axis, Lu designed a microfluidic device that reliably and robustly orients several hundred embryos in just a few minutes.

Details of the device design and results from proof-of-concept experiments with fruit fly embryos were published in the Dec. 26 advance online edition of the journal Nature Methods. This project was supported by the National Science Foundation, the National Institutes of Health, the Alfred P. Sloan Foundation and the DuPont Young Professor program.

Lu designed and fabricated the device with the help of Kwanghun Chung and Emily Gong, who worked on the project as Georgia Tech graduate and undergraduate students, respectively. Fabricated from polydimethylsiloxane (PDMS), the compact device is the size of a microscope slide and contains approximately 700 traps for embryos, which are shaped like grains of rice but smaller in size.

In operation, fluid flows through an "S"-shaped channel wide enough for embryos of any orientation to move easily through it. The fluid efficiently directs the embryos toward the traps, while sweeping out extra and improperly trapped embryos.

"The flow pattern significantly increased the frequency at which embryos contacted the traps and were loaded into them," explained Lu. "Experimentally, we found on average 90 percent of the embryos became trapped in the device, which will be valuable for studies that only have a small number of embryos available."

When an embryo approaches an empty trap, it experiences non‐uniform pressure and shear from the surrounding fluid. The resulting force flips the embryo vertically and inserts it into the cylindrical trap in an upright position, with its dorsoventral axis parallel to the ground. The embryo is then secured inside the trap, without any need for user intervention or control. The lock‐in feature allows the device to be disconnected from the rest of the hardware and transported for imaging or storage with the embryos enclosed.

"At one point, we mailed a microfluidic embryo trap array device full of trapped fruit fly embryos to our collaborators at Princeton University, and upon arrival, the embryos were still upright in their locked traps," said Lu.

To demonstrate the device's capabilities, Lu collaborated with Stanislav Shvartsman, an associate professor in the Department of Chemical and Biological Engineering at Princeton University, and his graduate student Yoosik Kim. The Princeton researchers used the device to quantify gradients of signaling molecules called morphogens in fixed embryos and also used it to monitor nuclear divisions in live embryos.

In one experiment, the Princeton researchers determined the spatial extent of the distribution of Dorsal, a transcription factor that initiates the dorsal‐to‐ventral patterning of the Drosophila embryo. They also demonstrated that this gradient could be quantitatively compared between wild-type and mutant embryos.

"The trap array device provided a significant increase in the number of fixed and live embryos we could image simultaneously and allowed us to accurately resolve issues of interest to developmental biologists today," explained Lu.

In the future, scientists should be able to adapt the microfluidic device for studies of pattern formation and morphogenesis in other model organisms, such as zebrafish or worm embryos. Results of those studies will be important to the scientific community because many genes controlling development are similar in worms, fruit flies and mammals.

This project was supported by the National Science Foundation (NSF) (Award No. DBI‐0649833) and the National Institutes of Health (NIH) (Award No. R21NS058465).


Story Source:

The above story is based on materials provided by Georgia Institute of Technology Research. Note: Materials may be edited for content and length.


Cite This Page:

Georgia Institute of Technology Research. "Microfluidic device rapidly orients hundreds of embryos for high-throughput experiments." ScienceDaily. ScienceDaily, 29 December 2010. <www.sciencedaily.com/releases/2010/12/101226131605.htm>.
Georgia Institute of Technology Research. (2010, December 29). Microfluidic device rapidly orients hundreds of embryos for high-throughput experiments. ScienceDaily. Retrieved December 21, 2014 from www.sciencedaily.com/releases/2010/12/101226131605.htm
Georgia Institute of Technology Research. "Microfluidic device rapidly orients hundreds of embryos for high-throughput experiments." ScienceDaily. www.sciencedaily.com/releases/2010/12/101226131605.htm (accessed December 21, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Sunday, December 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Researchers Test Colombian Village With High Alzheimer's Rates

Researchers Test Colombian Village With High Alzheimer's Rates

AFP (Dec. 19, 2014) In Yarumal, a village in N. Colombia, Alzheimer's has ravaged a disproportionately large number of families. A genetic "curse" that may pave the way for research on how to treat the disease that claims a new victim every four seconds. Duration: 02:42 Video provided by AFP
Powered by NewsLook.com
Monarch Butterflies Descend Upon Mexican Forest During Annual Migration

Monarch Butterflies Descend Upon Mexican Forest During Annual Migration

Reuters - Light News Video Online (Dec. 19, 2014) Millions of monarch butterflies begin to descend onto Mexico as part of their annual migration south. Rough Cut (no reporter narration) Video provided by Reuters
Powered by NewsLook.com
The Best Protein-Filled Foods to Energize You for the New Year

The Best Protein-Filled Foods to Energize You for the New Year

Buzz60 (Dec. 19, 2014) The new year is coming and nothing will energize you more for 2015 than protein-filled foods. Fitness and nutrition expert John Basedow (@JohnBasedow) gives his favorite high protein foods that will help you build muscle, lose fat and have endless energy. Video provided by Buzz60
Powered by NewsLook.com
Birds Might Be Better Meteorologists Than Us

Birds Might Be Better Meteorologists Than Us

Newsy (Dec. 19, 2014) A new study suggests a certain type of bird was able to sense a tornado outbreak that moved through the U.S. a day before it hit. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins