Featured Research

from universities, journals, and other organizations

A chaperone system guides tail-anchored membrane proteins to their destined membrane

Date:
July 5, 2011
Source:
Goethe University Frankfurt
Summary:
Newly synthesized proteins can only fold into their correct three dimensional structure thanks to chaperones. In case of membrane proteins chaperones do not only prevent their aggregation, but also escort them to their destination and aid in membrane insertion. The underlying molecular mechanism has now been resolved for tail-anchored membrane proteins.

Cristal structure of the Get3-receptor-complex. The two Get3 subunits are shown in green and blue. This is where the Get1 receptor-proteins (red and orange) bind. Upon interaction with its membrane receptor the Get3 dimer gradually opens up to allow for the controlled TA protein insertion.
Credit: Volker Dötsch

A newly synthesized protein is as fragile as a newborn baby. It could never fold into its correct three dimensional structure if it was not protected by chaperones within the densely populated cytosol. In case of membrane proteins chaperones do not only pre-vent their aggregation, but also escort them to their destination and aid in membrane insertion. The underlying molecular mechanism of how a certain family of membrane proteins is targeted and inserted into membranes has now been resolved by an international research team with participation of the Goethe University Frankfurt. These proteins are anchored within the membrane via a single helix and thus are called "tail-anchored" (TA) proteins.

The key for proper protein sorting are signal sequences which are decoded by chaperones. As soon as they -- together with their "foster child" -- arrive at their destination the interaction with a specific receptor within the target membrane initiates membrane insertion. Protein components responsible for the insertion of TA proteins have recently been identified. The molecular mechanisms of how these sorting systems work, however, were not known so far.

In this interdisciplinary study, recently published in the journal Science, the research groups of Prof.Volker Dötsch (Goethe University Frankfurt), Prof. Irmgard Sinning (Heidelberg University Biochemistry Center) and Prof. Vlad Denic (Harvard University (USA)) were able to solve the question by a combination of diverse methods such as X-Ray Crystallography and NMR-Spectroscopy as well as biochemical and cell-biological approaches.

In detailed biophysical studies Volker Dötsch´s research group showed that the central cha-peron of the responsible protein complex, called Get3, regulates both binding to TA proteins within the cytosol and their release at the membrane. The two receptor proteins Get1 and Get2 aid in TA protein insertion. They use overlapping interfaces for the interaction with the Get3 ATPase. On the basis of different crystal structures the researchers suggest a model for the mechanism of how TA proteins are inserted into the membrane. Upon interaction with its membrane receptor the Get3 dimer gradually opens up to allow for the controlled TA protein insertion. "Those results are particularly important because they enabled us to establish the first model of the receptor-assisted membrane insertion of TA proteins that will now be the basis for further studies" comments Dötsch.


Story Source:

The above story is based on materials provided by Goethe University Frankfurt. Note: Materials may be edited for content and length.


Journal Reference:

  1. S. Stefer, S. Reitz, F. Wang, K. Wild, Y.-Y. Pang, D. Schwarz, J. Bomke, C. Hein, F. Lohr, F. Bernhard, V. Denic, V. Dotsch, I. Sinning. Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex. Science, 2011; DOI: 10.1126/science.1207125

Cite This Page:

Goethe University Frankfurt. "A chaperone system guides tail-anchored membrane proteins to their destined membrane." ScienceDaily. ScienceDaily, 5 July 2011. <www.sciencedaily.com/releases/2011/07/110705104212.htm>.
Goethe University Frankfurt. (2011, July 5). A chaperone system guides tail-anchored membrane proteins to their destined membrane. ScienceDaily. Retrieved August 23, 2014 from www.sciencedaily.com/releases/2011/07/110705104212.htm
Goethe University Frankfurt. "A chaperone system guides tail-anchored membrane proteins to their destined membrane." ScienceDaily. www.sciencedaily.com/releases/2011/07/110705104212.htm (accessed August 23, 2014).

Share This




More Plants & Animals News

Saturday, August 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Endangered Red Wolves Face Uncertain Future

Endangered Red Wolves Face Uncertain Future

AP (Aug. 22, 2014) — A federal judge temporarily banned coyote hunting to save endangered red wolves, but local hunters say that the wolf preservation program does more harm than good. Meanwhile federal officials are reviewing its wolf program in North Carolina. (Aug. 22) Video provided by AP
Powered by NewsLook.com
Farm Resurgence Grows With Younger Crowd

Farm Resurgence Grows With Younger Crowd

AP (Aug. 22, 2014) — New England farms are seeing a surge in younger farm hands as the 'buy local' food movement grows across the country. (Aug. 22) Video provided by AP
Powered by NewsLook.com
Drug Used To Treat 'Ebola's Cousin' Shows Promise

Drug Used To Treat 'Ebola's Cousin' Shows Promise

Newsy (Aug. 21, 2014) — An experimental drug used to treat Marburg virus in rhesus monkeys could give new insight into a similar treatment for Ebola. Video provided by Newsy
Powered by NewsLook.com
Terrifying City-Dwelling Spiders Are Bigger And More Fertile

Terrifying City-Dwelling Spiders Are Bigger And More Fertile

Newsy (Aug. 21, 2014) — According to a new study, spiders that live in cities are bigger, fatter and multiply faster. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins