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How nitrous oxide is decomposed: Researchers identify structure of enzyme that breaks down potent greenhouse gas

Date:
August 22, 2011
Source:
Karlsruhe Institute of Technology
Summary:
Nitrous oxide is a harmful climate gas. Its effect as a greenhouse gas is 300 times stronger than that of carbon dioxide. Nitrous oxide destroys the ozone layer. In industrial agriculture, it is generated on excessively fertilized fields when microorganisms decompose nitrate fertilizers. Decomposition of nitrous oxide frequently is incomplete and strongly depends on environmental conditions. Researchers have now identified the structure of the enzyme that decomposes nitrous oxide and the decomposition mechanism.

Nitrous oxide (N2O) is a harmful climate gas. Its effect as a greenhouse gas is 300 times stronger than that of carbon dioxide. Nitrous oxide destroys the ozone layer. In industrial agriculture, it is generated on excessively fertilized fields when microorganisms decompose nitrate fertilizers. Decomposition of nitrous oxide frequently is incomplete and strongly depends on environmental conditions. Researchers from Freiburg, Constance, and KIT have now identified the structure of the enzyme that decomposes nitrous oxide and the decomposition mechanism.

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Their results are published in the journal Nature.

The study demonstrated that the N2O-reductase enzyme possesses active centers made up of four copper atoms and two sulfur atoms. "Surprisingly, we found that microbiologists all over the world have assumed an incorrect structure so far," explains Professor Oliver Einsle, group leader at the Institute of Organic Chemistry and Biochemistry of the University of Freiburg. Scientists have assumed a single sulfur atom only and have not been able to completely identify the nitrous oxide decomposition mechanism. Based on the new data, the reaction sequence of the enzyme can be modeled much better. Future investigations are to provide further details and help understand which influence environmental conditions have on the process.

"It was of decisive importance that all steps of our investigation were executed in the absence of air oxygen," emphasizes Walter G. Zumft, retired professor of Karlsruher Institute of Technology. In contact with oxygen, parts of the enzyme react and the enzyme changes its structure. Together with Dr. Anja Pomowski from the University of Freiburg, the bacteria were cultivated under an oxygen-free atmosphere, the enzymes were isolated on a large scale, crystallized, and the structure was analyzed using X-rays. The team of four authors was completed by Professor Peter Kroneck from the University of Constance.

"The current study provides interesting and complementary insight into the nitrogen cycle," says Dr. Ralf Kiese from the KIT Institute of Meteorology and Climate Research. Nitrous oxide and nitrogen production on fields, pastures, and in forests depends on a multitude of often opposing effects. Last year, a KIT study demonstrated that animal husbandry may lead to less nitrous oxide unter certain conditions (doi:10.1038/nature08931). Detailed knowledge of microbial processes and their dependence on environmental conditions might help to better model the nitrous oxide contribution to the climate. In the long term, it might even be feasible to use the knowledge in order to prevent nitrous oxide from being released into the atmosphere, for example, by additives in fertilizers that preserve the functioning of N2O-reductase or by optimized processes in sewage treatment plants.


Story Source:

The above story is based on materials provided by Karlsruhe Institute of Technology. Note: Materials may be edited for content and length.


Journal References:

  1. Anja Pomowski, Walter G. Zumft, Peter M. H. Kroneck, Oliver Einsle. N2O binding at a [4Cu:2S] copper–sulphur cluster in nitrous oxide reductase. Nature, 2011; DOI: 10.1038/nature10332
  2. Benjamin Wolf, Xunhua Zheng, Nicolas Brόggemann, Weiwei Chen, Michael Dannenmann, Xingguo Han, Mark A. Sutton, Honghui Wu, Zhisheng Yao, Klaus Butterbach-Bahl. Grazing-induced reduction of natural nitrous oxide release from continental steppe. Nature, 2010; 464 (7290): 881 DOI: 10.1038/nature08931

Cite This Page:

Karlsruhe Institute of Technology. "How nitrous oxide is decomposed: Researchers identify structure of enzyme that breaks down potent greenhouse gas." ScienceDaily. ScienceDaily, 22 August 2011. <www.sciencedaily.com/releases/2011/08/110822091605.htm>.
Karlsruhe Institute of Technology. (2011, August 22). How nitrous oxide is decomposed: Researchers identify structure of enzyme that breaks down potent greenhouse gas. ScienceDaily. Retrieved December 19, 2014 from www.sciencedaily.com/releases/2011/08/110822091605.htm
Karlsruhe Institute of Technology. "How nitrous oxide is decomposed: Researchers identify structure of enzyme that breaks down potent greenhouse gas." ScienceDaily. www.sciencedaily.com/releases/2011/08/110822091605.htm (accessed December 19, 2014).

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Aug. 15, 2011 — Researchers have discovered a new binding site for nitrous oxide (N2O). Nitrous oxide reductase, an enzyme containing copper, plays a key role in the biochemical process by ... read more

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