Featured Research

from universities, journals, and other organizations

Improved method for detecting mutant DNAs

Date:
October 11, 2011
Source:
Elsevier
Summary:
Molecular DNA testing methods offer clinicians powerful tools that serve to confirm or identify disease diagnoses. High sensitivity and high specificity, however, are frequently a challenge to achieve with these methods. Researchers now describe a new, robust technique that holds promise for identifying trace mutant DNA sequences (signals) in an overwhelming population of unmutated DNA (noise).

Molecular DNA testing methods offer clinicians powerful tools that serve to confirm or identify disease diagnoses. High sensitivity and high specificity, however, are frequently a challenge to achieve with these methods. In a study scheduled for publication in the November issue of The Journal of Molecular Diagnostics, researchers describe a new, robust technique that holds promise for identifying trace mutant DNA sequences (signals) in an overwhelming population of unmutated DNA (noise).

A group of researchers in Korea describe a simple and inexpensive enrichment technique that they have termed mutant enrichment with 3′-modified oligonucleotides (MEMO). This oligonucleotide blocks extension of the normal gene but enables extension of the mutated gene, allowing for increased detection sensitivity.

"The potential applications of MEMO include all situations in which minority alleles of clinical significance are present and sensitive detection is required," commented lead investigators Seung-Tae Lee MD, PhD, and Chang-Seok Ki, MD, PhD, Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. "In addition to the application of MEMO to detect cancer mutations, it can be used in other situations, such as variant strain identification in infectious diseases (for example, the YMDD mutation in hepatitis B virus infection and antiviral drug-resistant variants in human immunodeficiency virus infection), minor mutant allele detection in patients with low-level somatic mosaicism or mitochondrial heteroplasmy, and characterization of fetal mutations from maternal plasma samples."

Using genomic DNA extracted from cancer-derived cell lines containing EGFR, BRAF, JAK2, TP53, or KRAS mutations and from a bone marrow sample containing an NPM1 mutation, the authors were able to demonstrate significant sensitivity to these mutations.

Compared to preexisting methods, MEMO was shown to provide an improved diagnostic performance so that the method can be easily applicable in various medical fields, where molecular assays are important for disease diagnosis or treatment monitoring, and thus may help to improve patient outcomes.


Story Source:

The above story is based on materials provided by Elsevier. Note: Materials may be edited for content and length.


Journal Reference:

  1. Seung-Tae Lee, Ji-Youn Kim, Min-Jung Kown, Sun Wook Kim, Jae Hoon Chung, Myung-Ju Ahn, Young Lyun Oh, Jong-Won Kim, Chang-Seok Ki. Mutant Enrichment with 3′-Modified Oligonucleotides. The Journal of Molecular Diagnostics, 2011; DOI: 10.1016/j.jmoldx.2011.07.003

Cite This Page:

Elsevier. "Improved method for detecting mutant DNAs." ScienceDaily. ScienceDaily, 11 October 2011. <www.sciencedaily.com/releases/2011/10/111011112310.htm>.
Elsevier. (2011, October 11). Improved method for detecting mutant DNAs. ScienceDaily. Retrieved October 2, 2014 from www.sciencedaily.com/releases/2011/10/111011112310.htm
Elsevier. "Improved method for detecting mutant DNAs." ScienceDaily. www.sciencedaily.com/releases/2011/10/111011112310.htm (accessed October 2, 2014).

Share This



More Health & Medicine News

Thursday, October 2, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Pregnancy Spacing Could Have Big Impact On Autism Risks

Pregnancy Spacing Could Have Big Impact On Autism Risks

Newsy (Oct. 1, 2014) A new study says children born less than one year and more than five years after a sibling can have an increased risk for autism. Video provided by Newsy
Powered by NewsLook.com
Ebola Patient Told Hospital He Was from Liberia

Ebola Patient Told Hospital He Was from Liberia

AP (Oct. 1, 2014) The first Ebola patient diagnosed in the U.S. initially went to a Dallas emergency room last week but was sent home, despite telling a nurse that he had been in disease-ravaged West Africa, the hospital acknowledged Wednesday. (Oct. 1) Video provided by AP
Powered by NewsLook.com
Robotic Hair Restoration

Robotic Hair Restoration

Ivanhoe (Oct. 1, 2014) A new robotic procedure is changing the way we transplant hair. The ARTAS robot leaves no linear scarring and provides more natural results. Video provided by Ivanhoe
Powered by NewsLook.com
Insertable Cardiac Monitor

Insertable Cardiac Monitor

Ivanhoe (Oct. 1, 2014) A heart monitor the size of a paperclip that can save your life. The “Reveal Linq” allows a doctor to monitor patients with A-Fib on a continuous basis for up to 3 years! Video provided by Ivanhoe
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins