Featured Research

from universities, journals, and other organizations

UV light controls antibodies, improves biosensors

Date:
November 2, 2011
Source:
Optical Society of America
Summary:
From detecting pathogens in blood samples to the study of protein synthesis, Quartz Crystal Microbalance sensors have many uses in modern biology. Now researchers have found a way to increase the number of right-side-up antibodies in this well-established molecule detection process -- using light.

One UV photon is absorbed by the antibody and the disulfide bridge is opened, thereby forming thiol groups. Their interaction with the gold surface leads to an oriented Fab region so that the upside down position (circled in the right side of the picture) is hampered and the antigen binding is more effective.
Credit: Biomedical Optics Express.

From detecting pathogens in blood samples to the study of protein synthesis, Quartz Crystal Microbalance (QCM) sensors have many uses in modern biology. In this technique, antibodies anchored to gold electrodes on a piece of quartz crystal act like the "hooks" on the sticky side of a Velcro strap, grabbing molecules of interest as they pass by. The more molecule-sensing antibodies on the surface of the sensor, the more sensitive the QCM device's detection capabilities.

Unfortunately, some of the antibodies typically anchor themselves to the gold plate "hook"-side-down, rendering them useless as bio-receptors and dampening the sensor's sensitivity. Now researchers from the University of Naples "Federico II" and the Second University of Naples in Italy have found a way to increase the number of right-side-up antibodies in this well-established molecule detection process -- using light. In a paper recently published in the Optical Society's open-access journal Biomedical Optics Express, the team of scientists irradiated antibodies with ultra-short pulses of ultraviolet (UV) light.

The UV light is absorbed by the amino acid tryptophan, which breaks the disulfide bridges holding parts of the antibody together and causes a particular part of the amino acid cysteine, called a thiol group, to become exposed at the tail end of the antibody. Because thiol groups are more strongly attracted to the gold electrodes than other parts of the antibody, the bottom sides of these irradiated antibodies become much more likely to adhere to the gold electrodes than the "hook" ends. Using this method, the researchers were able to more than double the sensitivity of the QCM device, opening up new possibilities for research using this type of sensor, the researchers say.


Story Source:

The above story is based on materials provided by Optical Society of America. Note: Materials may be edited for content and length.


Journal Reference:

  1. Bartolomeo Della Ventura, Luigi Schiavo, Carlo Altucci, Rosario Esposito, Raffaele Velotta. Light assisted antibody immobilization for bio-sensing. Biomedical Optics Express, 2011; 2 (11): 3223 DOI: 10.1364/BOE.2.003223

Cite This Page:

Optical Society of America. "UV light controls antibodies, improves biosensors." ScienceDaily. ScienceDaily, 2 November 2011. <www.sciencedaily.com/releases/2011/10/111031121223.htm>.
Optical Society of America. (2011, November 2). UV light controls antibodies, improves biosensors. ScienceDaily. Retrieved July 31, 2014 from www.sciencedaily.com/releases/2011/10/111031121223.htm
Optical Society of America. "UV light controls antibodies, improves biosensors." ScienceDaily. www.sciencedaily.com/releases/2011/10/111031121223.htm (accessed July 31, 2014).

Share This




More Matter & Energy News

Thursday, July 31, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Britain Testing Driverless Cars on Roadways

Britain Testing Driverless Cars on Roadways

AP (July 30, 2014) British officials said on Wednesday that driverless cars will be tested on roads in as many as three cities in a trial program set to begin in January. Officials said the tests will last up to three years. (July 30) Video provided by AP
Powered by NewsLook.com
Amid Drought, UCLA Sees Only Water

Amid Drought, UCLA Sees Only Water

AP (July 30, 2014) A ruptured 93-year-old water main left the UCLA campus awash in 8 million gallons of water in the middle of California's worst drought in decades. (July 30) Video provided by AP
Powered by NewsLook.com
Smartphone Powered Paper Plane Debuts at Airshow

Smartphone Powered Paper Plane Debuts at Airshow

AP (July 30, 2014) Smartphone powered paper airplane that was popular on crowdfunding website KickStarter makes its debut at Wisconsin airshow (July 30) Video provided by AP
Powered by NewsLook.com
U.K. To Allow Driverless Cars On Public Roads

U.K. To Allow Driverless Cars On Public Roads

Newsy (July 30, 2014) Driverless cars could soon become a staple on U.K. city streets, as they're set to be introduced to a few cities in 2015. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

    Technology News



      Save/Print:
      Share:

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile: iPhone Android Web
      Follow: Facebook Twitter Google+
      Subscribe: RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins