Featured Research

from universities, journals, and other organizations

UV light controls antibodies, improves biosensors

Date:
November 2, 2011
Source:
Optical Society of America
Summary:
From detecting pathogens in blood samples to the study of protein synthesis, Quartz Crystal Microbalance sensors have many uses in modern biology. Now researchers have found a way to increase the number of right-side-up antibodies in this well-established molecule detection process -- using light.

One UV photon is absorbed by the antibody and the disulfide bridge is opened, thereby forming thiol groups. Their interaction with the gold surface leads to an oriented Fab region so that the upside down position (circled in the right side of the picture) is hampered and the antigen binding is more effective.
Credit: Biomedical Optics Express.

From detecting pathogens in blood samples to the study of protein synthesis, Quartz Crystal Microbalance (QCM) sensors have many uses in modern biology. In this technique, antibodies anchored to gold electrodes on a piece of quartz crystal act like the "hooks" on the sticky side of a Velcro strap, grabbing molecules of interest as they pass by. The more molecule-sensing antibodies on the surface of the sensor, the more sensitive the QCM device's detection capabilities.

Related Articles


Unfortunately, some of the antibodies typically anchor themselves to the gold plate "hook"-side-down, rendering them useless as bio-receptors and dampening the sensor's sensitivity. Now researchers from the University of Naples "Federico II" and the Second University of Naples in Italy have found a way to increase the number of right-side-up antibodies in this well-established molecule detection process -- using light. In a paper recently published in the Optical Society's open-access journal Biomedical Optics Express, the team of scientists irradiated antibodies with ultra-short pulses of ultraviolet (UV) light.

The UV light is absorbed by the amino acid tryptophan, which breaks the disulfide bridges holding parts of the antibody together and causes a particular part of the amino acid cysteine, called a thiol group, to become exposed at the tail end of the antibody. Because thiol groups are more strongly attracted to the gold electrodes than other parts of the antibody, the bottom sides of these irradiated antibodies become much more likely to adhere to the gold electrodes than the "hook" ends. Using this method, the researchers were able to more than double the sensitivity of the QCM device, opening up new possibilities for research using this type of sensor, the researchers say.


Story Source:

The above story is based on materials provided by Optical Society of America. Note: Materials may be edited for content and length.


Journal Reference:

  1. Bartolomeo Della Ventura, Luigi Schiavo, Carlo Altucci, Rosario Esposito, Raffaele Velotta. Light assisted antibody immobilization for bio-sensing. Biomedical Optics Express, 2011; 2 (11): 3223 DOI: 10.1364/BOE.2.003223

Cite This Page:

Optical Society of America. "UV light controls antibodies, improves biosensors." ScienceDaily. ScienceDaily, 2 November 2011. <www.sciencedaily.com/releases/2011/10/111031121223.htm>.
Optical Society of America. (2011, November 2). UV light controls antibodies, improves biosensors. ScienceDaily. Retrieved March 1, 2015 from www.sciencedaily.com/releases/2011/10/111031121223.htm
Optical Society of America. "UV light controls antibodies, improves biosensors." ScienceDaily. www.sciencedaily.com/releases/2011/10/111031121223.htm (accessed March 1, 2015).

Share This


More From ScienceDaily



More Matter & Energy News

Sunday, March 1, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Rehab Robot Helps Restore Damaged Muscles and Nerves

Rehab Robot Helps Restore Damaged Muscles and Nerves

Reuters - Innovations Video Online (Mar. 1, 2015) A rehabilitation robot prototype to help restore deteriorated nerves and muscles using electromyography and computer games. Ben Gruber reports. Video provided by Reuters
Powered by NewsLook.com
Elon Musk's Hyperloop Moves Forward

Elon Musk's Hyperloop Moves Forward

Buzz60 (Feb. 27, 2015) Zipping around at 800-miles an hour is coming closer to reality in California. An entire town is being built around Elon Musk&apos;s Hyperloop concept and it wants you to stop in for a ride when it&apos;s ready. Brett Larson is on board. Video provided by Buzz60
Powered by NewsLook.com
Vibrating Bicycle Senses Traffic

Vibrating Bicycle Senses Traffic

Reuters - Innovations Video Online (Feb. 26, 2015) Dutch scientists have developed a smart bicycle that uses sensors, wireless technology and video to warn riders of traffic dangers. Ben Gruber reports. Video provided by Reuters
Powered by NewsLook.com
In Japan, Robot Dogs Are for Life -- And Death

In Japan, Robot Dogs Are for Life -- And Death

AFP (Feb. 25, 2015) Robot dogs are the perfect pet for some in Japan who go to repairmen-turned-vets when their pooch breaks down - while a full Buddhist funeral ceremony awaits those who don&apos;t make it. Duration: 02:40 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins