Featured Research

from universities, journals, and other organizations

DNA mismatch repair happens only during a brief window of opportunity

Date:
January 6, 2012
Source:
University of California - San Diego
Summary:
In eukaryotes-the group of organisms that include humans –- a key to survival is the ability of certain proteins to quickly and accurately repair genetic errors that occur when DNA is replicated to make new cells. Researchers have solved part of the mystery of how these proteins do their job, a process called DNA mismatch repair.

In eukaryotes -- the group of organisms that include humans -- a key to survival is the ability of certain proteins to quickly and accurately repair genetic errors that occur when DNA is replicated to make new cells.

Related Articles


In a paper published in the December 23, 2011 issue of the journal Science, researchers at the Ludwig Institute for Cancer Research and the University of California, San Diego School of Medicine have solved part of the mystery of how these proteins do their job, a process called DNA mismatch repair (MMR).

"One of the major questions in MMR is how MMR proteins figure out which base in a DNA mispair is the wrong one," said Ludwig Institute assistant investigator Christopher D. Putnam, PhD, an adjunct assistant professor of medicine at UC San Diego. "For example, if guanine (G) is inappropriately in a base-pair with thymine (T), is the G or the T the error? Picking the wrong base results in mutations, not fixes."

Using Saccharomyces cerevisiae, or baker's yeast, as their model organism, the researchers, led by Richard D. Kolodner, PhD, Ludwig Institute investigator and UCSD professor of medicine and cellular and molecular medicine, discovered that newly replicated DNA produces a temporary signal for 10 to 15 minutes after replication which helps identify it as new -- and thus a potential subject for MMR.

The actual signal was not identified, but Putnam said it might be tell-tale nicks in single-stranded DNA or certain proteins associated with replication. The scientists are working to pinpoint the precise signal.

The findings, combined with earlier, published work that visualized MMR in a living cell for the first time, more fully explains how eukaryotes eliminate DNA replication errors, which can result in defects and the development of cancers.

"How eukaryotes identify the newly synthesized strand of DNA is a mystery that has persisted for at least 30 years," said Putnam. "These findings really change our ideas of how MMR works," said Putnam.

Co-authors include Hans Hombauer and Anjana Srivatsan of the Ludwig Institute for Cancer Research, UCSD Departments of Medicine and Cellular and Molecular Medicine, Institute of Genomic Medicine and UC San Diego Moores Cancer Center.

Funding for this research came from the National Institutes of Health.


Story Source:

The above story is based on materials provided by University of California - San Diego. Note: Materials may be edited for content and length.


Journal Reference:

  1. H. Hombauer, A. Srivatsan, C. D. Putnam, R. D. Kolodner. Mismatch Repair, But Not Heteroduplex Rejection, Is Temporally Coupled to DNA Replication. Science, 2011; 334 (6063): 1713 DOI: 10.1126/science.1210770

Cite This Page:

University of California - San Diego. "DNA mismatch repair happens only during a brief window of opportunity." ScienceDaily. ScienceDaily, 6 January 2012. <www.sciencedaily.com/releases/2011/12/111222142454.htm>.
University of California - San Diego. (2012, January 6). DNA mismatch repair happens only during a brief window of opportunity. ScienceDaily. Retrieved December 18, 2014 from www.sciencedaily.com/releases/2011/12/111222142454.htm
University of California - San Diego. "DNA mismatch repair happens only during a brief window of opportunity." ScienceDaily. www.sciencedaily.com/releases/2011/12/111222142454.htm (accessed December 18, 2014).

Share This


More From ScienceDaily



More Health & Medicine News

Thursday, December 18, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Kids Die While Under Protective Services

Kids Die While Under Protective Services

AP (Dec. 18, 2014) As part of a six-month investigation of child maltreatment deaths, the AP found that hundreds of deaths from horrific abuse and neglect could have been prevented. AP's Haven Daley reports. (Dec. 18) Video provided by AP
Powered by NewsLook.com
UN: Up to One Million Facing Hunger in Ebola-Hit Countries

UN: Up to One Million Facing Hunger in Ebola-Hit Countries

AFP (Dec. 17, 2014) Border closures, quarantines and crop losses in West African nations battling the Ebola virus could lead to as many as one million people going hungry, UN food agencies said on Wednesday. Duration: 00:52 Video provided by AFP
Powered by NewsLook.com
When You Lose Weight, This Is Where The Fat Goes

When You Lose Weight, This Is Where The Fat Goes

Newsy (Dec. 17, 2014) Can fat disappear into thin air? New research finds that during weight loss, over 80 percent of a person's fat molecules escape through the lungs. Video provided by Newsy
Powered by NewsLook.com
Why Your Boss Should Let You Sleep In

Why Your Boss Should Let You Sleep In

Newsy (Dec. 17, 2014) According to research out of the University of Pennsylvania, waking up for work is the biggest factor that causes Americans to lose sleep. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins