Featured Research

from universities, journals, and other organizations

Researchers collect and reuse enzymes while maintaining bioactivity

Date:
August 8, 2012
Source:
Clemson University
Summary:
Researchers are collecting and harvesting enzymes while maintaining the enzyme's bioactivity. The new model system may impact cancer research.

Clemson University researchers are collecting and harvesting enzymes while maintaining the enzyme's bioactivity. Their work, a new model system that may impact cancer research, is published in the journal Small.

Related Articles


Enzymes are round proteins produced by living organisms that increase the rate of chemical reactions.

"We found a robust and simple way of attracting specific enzymes, concentrating them and reusing them," said Stephen Foulger, professor in the School of Materials Science and Engineering at Clemson. "The enzymes are still functional after being harvested."

Isolating a single type of protein from a complex mixture is the most difficult aspect of the purification process. It is vital to determine the function, structure and interactions of the protein.

The researchers baited a nanoparticle to capture and recycle an enzyme. They found a way to attach an enzyme's target on the surface of a particle, allow the enzyme to bind to it, remove the particle and determine that the enzyme is still functional.

"We took a protein that was being produced in a soil and placed its food source on the outside of a nanoparticle and the protein essentially grabbed onto the food source," said Foulger. "We froze the enzyme in place and removed the particle and thus found a commercially viable way to harvest these proteins."

"This baited particle approach provides a very efficient means for isolating complex enzyme systems for use in biotechnology," said Vincent Rotello, a chemistry professor at the University of Massachusetts Amherst and leading researcher in the field. "This method also provides considerable promise for biomedical applications."

The research established a universal model for concentrating and extracting known enzyme pairings, but it can be an invaluable tool in recognizing unknown ones.

"This model is foreshadowing for what we're doing with cancer research because we're beginning to focus on the 'outside' of nanoparticles to sequester specific proteins that direct cancer cell growth," said Foulger.

The researchers' goal is to alter the cellular concentration of critical proteins in cancer to disrupt the cell's ability to spread, thereby controlling its growth in the body.


Story Source:

The above story is based on materials provided by Clemson University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Michael A. Daniele, Yuriy P. Bandera, Deepti Sharma, Parul Rungta, Ryan Roeder, Michael G. Sehorn, Stephen H. Foulger. Substrate-Baited Nanoparticles: A Catch and Release Strategy for Enzyme Recognition and Harvesting. Small, 2012; 8 (13): 2083 DOI: 10.1002/smll.201200013

Cite This Page:

Clemson University. "Researchers collect and reuse enzymes while maintaining bioactivity." ScienceDaily. ScienceDaily, 8 August 2012. <www.sciencedaily.com/releases/2012/08/120808132721.htm>.
Clemson University. (2012, August 8). Researchers collect and reuse enzymes while maintaining bioactivity. ScienceDaily. Retrieved December 21, 2014 from www.sciencedaily.com/releases/2012/08/120808132721.htm
Clemson University. "Researchers collect and reuse enzymes while maintaining bioactivity." ScienceDaily. www.sciencedaily.com/releases/2012/08/120808132721.htm (accessed December 21, 2014).

Share This


More From ScienceDaily



More Matter & Energy News

Sunday, December 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Building Google Into Cars

Building Google Into Cars

Reuters - Business Video Online (Dec. 19, 2014) Google's next Android version could become the standard that'll power your vehicle's entertainment and navigation features, Reuters has learned. Fred Katayama reports. Video provided by Reuters
Powered by NewsLook.com
AP Review: Nikon D750 and GoPro Hero 4

AP Review: Nikon D750 and GoPro Hero 4

AP (Dec. 19, 2014) What to buy an experienced photographer or video shooter? There is some strong gear on the market from Nikon and GoPro. The AP's Ron Harris takes a closer look. (Dec. 19) Video provided by AP
Powered by NewsLook.com
Double-Amputee Becomes First To Move Two Prosthetic Arms With His Mind

Double-Amputee Becomes First To Move Two Prosthetic Arms With His Mind

Buzz60 (Dec. 19, 2014) A double-amputee makes history by becoming the first person to wear and operate two prosthetic arms using only his mind. Jen Markham has the story. Video provided by Buzz60
Powered by NewsLook.com
Navy Unveils Robot Fish

Navy Unveils Robot Fish

Reuters - Light News Video Online (Dec. 18, 2014) The U.S. Navy unveils an underwater device that mimics the movement of a fish. Tara Cleary reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins