Featured Research

from universities, journals, and other organizations

Multi-talented enzyme produced on large-scale

Date:
October 24, 2012
Source:
Fraunhofer-Gesellschaft
Summary:
Enzymes remove spots from our laundry, whiten paper and help with brewing beer. To wit: They facilitate many industrial processes. In many cases, enzymes are obtained from fruits. However, if the harvest is poor, this might lead to shortfalls. Researchers therefore designed a process to produce enzymes microbiologically. A new multifunctional facility should help with adapting these new processes to an industrial scale.

The new fermentation plant transforms the processes of producing enzymes to industrial scale.
Credit: Fraunhofer

Enzymes remove spots from our laundry, whiten paper and help with brewing beer. To wit: They facilitate many industrial processes. In many cases, enzymes are obtained from fruits. However, if the harvest is poor, this might lead to shortfalls. Researchers therefore designed a process to produce enzymes microbiologically.

A multifunctional facility now opening in Leuna should help with adapting these new processes to an industrial scale.

Papayas are delicious and healthy -- and they contain papain, an enzyme that is isolated from the fruit and used in countless industries. They are used for brewing beer, the handling of meat products, the treating of wool in the textile industry and the treating of inflammation in the medical field. A similar example is represented by horseradish: Its enzyme -- horseradish peroxidase -- is used in large quantities in several diagnostic and immunological tests. But the horseradish harvest varies dramatically from year to year. When the harvest is poor, then horseradish peroxidase is no longer available in quantities that the industry needs.

For this reason, the researchers within the "Innozym" project at the Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB in Stuttgart are designing pathways to manufacture enzymes using biotechnology with the aid of microorganisms. The advantage: No matter how the harvest turns out, enzymes can always be sufficiently produced. In addition, the scientists are also creating entirely new enzymes that are intended to replace chemical catalysts. These could make it possible to run industrial operations at lower temperatures and thereby save energy. They could also reduce the usage of process chemicals, such as those used to modulate pH values. In the laboratories, the researchers are producing the enzymes in reaction vessels that can hold a maximum of 30 liters. In the factory, by contrast, the production reactions for enzymes actually start at 10,000 liters. However, one cannot simply apply the same production steps that work for small batches of enzymes to large batches. Even though researchers at the lab could, for example, add expensive substances, this would make large-scale production economically infeasible.

The Fraunhofer Center for Chemical-Biotechnological Processes CBP in Leuna -- which belongs to the Fraunhofer IGB and Fraunhofer Institute for Chemical Technologies ICT -- is expected to close this gap between laboratory and industry. A multifunctional facility has also been integrated into the new building that is set to open in October. "We are scaling the processes that our colleagues at the IGB developed and optimized in the laboratory -- from the 10-liter laboratory-scale to 10,000 liters high. That is often technically a quite different process," says Dr. Katja Patzsch, group manager of biotechnological processes at CBP. "The emerging multifunctional facility is unlike anything else in Europe." It was designed and built by the company Linde Engineering Dresden GmbH.

Generally speaking, the manner in which the enzymes are produced remains the same -- no matter if large or small batches of enzymes are produced. The researchers are cultivating certain organisms in a culture medium, like yeast cells or bacteria. If sufficient organisms were cultivated, the scientists add an inductor -- a substance that stimulates the cells or bacteria to produce the desired enzyme, and then transfer it into the culture medium. The researchers first extract the cells from the fluid. In this respect, the separator can handle any quantity of fluid up to 10,000 liters. In a later step, the scientists fish out the enzyme from the residual suspension -- for instance, by crystallizing or filtering the enzyme, or separating it through a chromatography column.

The "Innozym" project

The "Innozym" project was funded by the German Federal Ministry of Education and Research BMBF. Beside Fraunhofer IGB, project partners also include the Institute for Interfacial Engineering IGVT at the University of Stuttgart as well as the companies c-LEcta GmbH, InfraLeuna GmbH and Linde Engineering Dresden GmbH. A component of the sponsorship also includes the multifunctional facility that Linde Engineering Dresden GmbH constructed as a "Technical Enzyme" module at Fraunhofer CBP. With this module, enzymes, biotransformation processes and the actual enzyme production -- including the reprocessing in bacteria and yeast -- can be optimized.


Story Source:

The above story is based on materials provided by Fraunhofer-Gesellschaft. Note: Materials may be edited for content and length.


Cite This Page:

Fraunhofer-Gesellschaft. "Multi-talented enzyme produced on large-scale." ScienceDaily. ScienceDaily, 24 October 2012. <www.sciencedaily.com/releases/2012/10/121024101648.htm>.
Fraunhofer-Gesellschaft. (2012, October 24). Multi-talented enzyme produced on large-scale. ScienceDaily. Retrieved October 23, 2014 from www.sciencedaily.com/releases/2012/10/121024101648.htm
Fraunhofer-Gesellschaft. "Multi-talented enzyme produced on large-scale." ScienceDaily. www.sciencedaily.com/releases/2012/10/121024101648.htm (accessed October 23, 2014).

Share This



More Plants & Animals News

Thursday, October 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Working Mother DIY: Pumpkin Pom-Pom

Working Mother DIY: Pumpkin Pom-Pom

Working Mother (Oct. 22, 2014) How to make a pumpkin pom-pom. Video provided by Working Mother
Powered by NewsLook.com
San Diego Zoo's White Rhinos Provide Hope for the Critically Endangered Species

San Diego Zoo's White Rhinos Provide Hope for the Critically Endangered Species

Reuters - Light News Video Online (Oct. 22, 2014) The pair of rare white northern rhinos bring hope for their species as only six remain in the world. Elly Park reports. Video provided by Reuters
Powered by NewsLook.com
Raw: Bear Cub Strolls Through Oregon Drug Store

Raw: Bear Cub Strolls Through Oregon Drug Store

AP (Oct. 22, 2014) Shoppers at an Oregon drug store were surprised by a bear cub scurrying down the aisles this past weekend. (Oct. 22) Video provided by AP
Powered by NewsLook.com
Family Pleads for Pet Pig to Stay at Home

Family Pleads for Pet Pig to Stay at Home

AP (Oct. 22, 2014) The Johnson family lost their battle with the Chesterfield County, Virginia Planning Commission to allow Tucker, their pet pig, to stay in their home, but refuse to let the board keep Tucker away. (Oct. 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins