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Protein folding via charge zippers

Date:
January 18, 2013
Source:
Karlsruhe Institute of Technology
Summary:
Membrane proteins are the “molecular machines” in biological cell envelopes. They control diverse processes, such as the transport of molecules across the lipid membrane, signal transduction, and photosynthesis. Their shape, i.e. folding of the molecules, plays a decisive role in the formation of, e.g., pores in the cell membrane. Scientists are now reporting a novel charge zipper principle used by proteins to form functional units.

Like the teeth of a zipper, the charged amino acids (red, blue) form connections between protein segments. In this way, they can form pores in the cell membrane.
Credit: Figure: KIT

Membrane proteins are the "molecular machines" in biological cell envelopes. They control diverse processes, such as the transport of molecules across the lipid membrane, signal transduction, and photosynthesis. Their shape, i.e. folding of the molecules, plays a decisive role in the formation of, e.g., pores in the cell membrane. In the journal Cell, researchers of Karlsruhe Institute of Technology and the University of Cagliari are now reporting a novel charge zipper principle used by proteins to form functional units.

"It is fascinating to see the elegant basic principles that are used by nature to construct molecular assemblies," explains Anne Ulrich, Director of the KIT Institute for Biological Interfaces. "A charge zipper between the charged side chains is an entirely unexpected mechanism used by membrane proteins to neutralize their charges such that they can be immersed into hydrophobic cell membranes."

In the study published now, Ulrich and her team investigate the so-called Twin-arginine translocase (Tat) that is used in the cell membrane of bacteria as an export machinery for folded proteins. Several TatA subunits assemble as a pore that can adapt its diameter to the size of the cargo to be transported. "But how can such a pore be built up from TatA proteins? How can they reversibly form a huge hole in the membrane for a variety of molecules to pass through, but without causing leakage of the cell?," Ulrich formulates the questions studied.

To answer these questions, the researchers studied the molecular structure of TatA protein from the bacterium B. subtilis, which consists of a chain of 70 amino acids. The analysis showed that it folds into a rather rigid, rod-shaped helix that is followed by a flexible, extended stretch. Many amino acids in the helix and the adjacent stretch carry positive or negative charges. Surprisingly, the sequence of charges on the helix is complementary to those in the adjacent stretch of the protein. When the protein is folded up at the connection point like a pocket knife, positive and negative charges will always meet and attract each other. Hence, the protein links up both of its segments, similar to the interlocking teeth of a zipper.

"The clou is that this binding principle also works with the neighboring proteins," Ulrich says. Instead of folding up alone, every TatA protein also forms charge zippers with both of its neighbors. Computer simulations showed that this leads to stable and, at the same time, flexible connections between the adjacent molecules. In this way, any number of proteins can be linked together to form an uncharged ring, which thus lines the TatA pore in the hydrophobic membrane. This novel charge zipper principle does not only seem to play a role in protein transport, but also in the attack of certain antimicrobial peptides on bacteria, or in their formation of biofilms as a response to stress.


Story Source:

The above story is based on materials provided by Karlsruhe Institute of Technology. Note: Materials may be edited for content and length.


Journal Reference:

  1. TorstenH. Walther, Christina Gottselig, StephanL. Grage, Moritz Wolf, AttilioV. Vargiu, MarcoJ. Klein, Stefanie Vollmer, Sebastian Prock, Mareike Hartmann, Sergiy Afonin, Eva Stockwald, Hartmut Heinzmann, OlgaV. Nolandt, Wolfgang Wenzel, Paolo Ruggerone, AnneS. Ulrich. Folding and Self-Assembly of the TatA Translocation Pore Based on a Charge Zipper Mechanism. Cell, 2013; 152 (1-2): 316 DOI: 10.1016/j.cell.2012.12.017

Cite This Page:

Karlsruhe Institute of Technology. "Protein folding via charge zippers." ScienceDaily. ScienceDaily, 18 January 2013. <www.sciencedaily.com/releases/2013/01/130118111559.htm>.
Karlsruhe Institute of Technology. (2013, January 18). Protein folding via charge zippers. ScienceDaily. Retrieved August 27, 2014 from www.sciencedaily.com/releases/2013/01/130118111559.htm
Karlsruhe Institute of Technology. "Protein folding via charge zippers." ScienceDaily. www.sciencedaily.com/releases/2013/01/130118111559.htm (accessed August 27, 2014).

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