Featured Research

from universities, journals, and other organizations

New method of mass-producing high-quality DNA molecules

Date:
June 2, 2013
Source:
Karolinska Institutet
Summary:
A new method of manufacturing short, single-stranded DNA molecules can solve many of the problems associated with current production methods. The new method can be of value to both DNA nanotechnology and the development of drugs consisting of DNA fragments.

This shows Cosimo Ducani, postdoc, and Björn Högberg, PI, in their lab at Karolinska Institutet in Stockholm, Sweden.
Credit: Karolinska Institutet (Creative Commons Licensed)

A new method of manufacturing short, single-stranded DNA molecules can solve many of the problems associated with current production methods. The new method, which is described in the scientific periodical Nature Methods, can be of value to both DNA nanotechnology and the development of drugs consisting of DNA fragments.

Related Articles


The novel technique for manufacturing short, single-stranded DNA molecules -- or oligonucleotides -- has been developed by researchers at Karolinska Institutet in Sweden and Harvard University. Such DNA fragments constitute a basic tool for researchers and play a key part in many fields of science. Many of the recent advances in genetic and molecular biological research and development, such as the ability to quickly scan an organism's genome, would not have been possible without oligonucleotides.

The new method is versatile and able to solve problems that currently restrict the production of DNA fragments.

"We've used enzymatic production methods to create a system that not only improves the quality of the manufactured oligonucleotides but that also makes it possible to scale up production using bacteria in order to produce large amounts of DNA copies cheaply," says co-developer Björn Högberg at the Swedish Medical Nanoscience Center, part of the Department of Neuroscience at Karolinska Institutet in Sweden.

The process of bioproduction, whereby bacteria are used to copy DNA sequences, enables the manufacture of large amounts of DNA copies at a low cost. Unlike current methods of synthesising oligonucleotides, where the number of errors increases with the length of the sequence, this new method according to the developers also works well for long oligonucleotides of several hundred nitrogenous bases.

The DNA molecules are first formed as a long string of single-stranded DNA in which the sequence of interest is repeated several times. The long strand forms tiny regions called hairpins, where the strand folds back on itself. These hairpins can then be cut up by enzymes, which serve as a molecular-biological pair of scissors that cuts the DNA at selected sites. Several different oligonucleotides can thus be produced at the same time in a perfectly balanced combination, which is important if they are to be crystallised or used therapeutically.

"Oligonucleotide-based drugs are already available, and it's very possible that our method could be used to produce purer and cheaper versions of these drugs," says Dr Björn Högberg.

The project was financed by grants from the Swedish Research Council, the Swedish governmental agency for innovation systems (Vinnova) and Carl Bennet AB.


Story Source:

The above story is based on materials provided by Karolinska Institutet. Note: Materials may be edited for content and length.


Journal Reference:

  1. Cosimo Ducani, Corinna Kaul, Martin Moche, William M Shih, Björn Högberg. Enzymatic production of 'monoclonal stoichiometric' single-stranded DNA oligonucleotides. Nature Methods, 2013; DOI: 10.1038/nmeth.2503

Cite This Page:

Karolinska Institutet. "New method of mass-producing high-quality DNA molecules." ScienceDaily. ScienceDaily, 2 June 2013. <www.sciencedaily.com/releases/2013/06/130602144454.htm>.
Karolinska Institutet. (2013, June 2). New method of mass-producing high-quality DNA molecules. ScienceDaily. Retrieved April 21, 2015 from www.sciencedaily.com/releases/2013/06/130602144454.htm
Karolinska Institutet. "New method of mass-producing high-quality DNA molecules." ScienceDaily. www.sciencedaily.com/releases/2013/06/130602144454.htm (accessed April 21, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Tuesday, April 21, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Blue Bell Recalls All Products

Blue Bell Recalls All Products

AP (Apr. 21, 2015) — Blue Bell Creameries voluntary recalled for all of its products after two samples of chocolate chip cookie dough ice cream tested positive for listeria, a potentially deadly bacteria. Blue Bell&apos;s President and CEO issued a video statement. (April 21) Video provided by AP
Powered by NewsLook.com
'Tutu Tuesdays' Brighten Faces at Kids' Hospital

'Tutu Tuesdays' Brighten Faces at Kids' Hospital

AP (Apr. 21, 2015) — Doctors and nurses have started wearing ballet tutus every Tuesday to cheer up young hospital patients at a Florida hospital. It started with a request made by a nervous patient -- now, almost the entire staff is wearing the tutus. (April 21) Video provided by AP
Powered by NewsLook.com
Humanoid Robot Can Recognise and Interact With People

Humanoid Robot Can Recognise and Interact With People

Reuters - Innovations Video Online (Apr. 20, 2015) — An ultra-realistic humanoid robot called &apos;Han&apos; recognises and interprets people&apos;s facial expressions and can even hold simple conversations. Developers Hanson Robotics hope androids like Han could have uses in hospitality and health care industries where face-to-face communication is vital. Matthew Stock reports. Video provided by Reuters
Powered by NewsLook.com
Labour Party Warns Britain's Health Service 'on Life Support'

Labour Party Warns Britain's Health Service 'on Life Support'

AFP (Apr. 20, 2015) — Britain&apos;s opposition Labour Party Monday claimed the National Health Service (NHS) was &apos;on life support&apos; as it turned its attention to the state-run service, which is a key issue for the UK&apos;s May 7 general election. Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins