Featured Research

from universities, journals, and other organizations

New species of fish pathogenic bacterium Edwardsiella

Date:
December 5, 2013
Source:
Norwegian School of Veterinary Science
Summary:
A doctoral research project has uncovered a genetic divergence between the fish pathogen Edwardsiella tarda and Edwardsiella tarda type strain. It has also identified phenotypic markers that distinguish one from the other. The fish pathogenic strain is now classified as a separate species: Edwardsiella piscicida.

Electronic micrograph of E. Tarda (now E. piscicida) LTB4 strain after negative staining.
Credit: Courtesy of Lan et al. 2008

Takele Abayneh Tefera's doctoral research project has uncovered a genetic divergence between the fish pathogen Edwardsiella tarda and Edwardsiella tarda type strain. He has also identified phenotypic markers that distinguish one from the other. The fish pathogenic strain is now classified as a separate species: Edwardsiella piscicida.

Edwardsiella tarda is a bacterium that can infect a number of animal species and also humans. Edwardsiellosis is one of the most serious systemic bacterial diseases in fish, resulting in substantial losses in the fish farming industry all over the world.

Takele Abayneh Tefera has developed effective molecular tools for the identification and characterization of different strains of Edwardsiella. He developed a new TaqMan real-time and conventional PCR analysis for this purpose and then evaluated it in relation to the Loop Mediated Isothermal Amplification (LAMP) analysis. For the first time, he also used the Multi-locus Sequence Analysis (MLSA) for the typing and characterization of E. tarda, isolated from different sources. This is a useful tool for detecting sources of infection and for understanding the epidemiological relationship between isolates from the environment, fish, livestock and humans.

The MLSA analysis showed that there exist geographic and host-specific genotypes of the species E. tarda. Bacterial strains from fish were found to be only distantly related to E. tarda type strain and other strains from humans. Furthermore, genetic and phenotypic analyses also confirmed a genetic divergence between these strains of bacteria.

The findings of this study indicate that the fish pathogenic strain has been wrongly classified as E. tarda. Phenotypic characterization by means of a number of biochemical tests and pathogenity studies on zebra fish identified phenotypic markers which also differentiate fish pathogenic strains from the reference bacterium.

Based on genetic and phenotypic differences, Tefera and his colleagues have proposed that fish pathogenic Edwardsiella strains previously classified as Edwardsiella tarda should now be classified as a new species: Edwardsiella piscicida. The new species has now been approved and incorporated into prokaryotic nomenclature.

Tefera's work also led to the introduction of a new Multi-locus Variable Number Tandem Repeat Analysis (MLVA) for the further typing of different isolates of the new species of bacteria Edwardsiella piscicida. Employing this method proved to be more sensitive than the MLSA-analysis when it came to investigating outbreaks of E. piscicida.

Takele Abayneh Tefera defended his doctoral research on 26th November 2013 at the Norwegian School of Veterinary Science with a thesis entitled: "Fish pathogenic Edwardsiella tarda: Evaluation of molecular identification methods and characterization of a novel species."


Story Source:

The above story is based on materials provided by Norwegian School of Veterinary Science. Note: Materials may be edited for content and length.


Cite This Page:

Norwegian School of Veterinary Science. "New species of fish pathogenic bacterium Edwardsiella." ScienceDaily. ScienceDaily, 5 December 2013. <www.sciencedaily.com/releases/2013/12/131205092051.htm>.
Norwegian School of Veterinary Science. (2013, December 5). New species of fish pathogenic bacterium Edwardsiella. ScienceDaily. Retrieved September 21, 2014 from www.sciencedaily.com/releases/2013/12/131205092051.htm
Norwegian School of Veterinary Science. "New species of fish pathogenic bacterium Edwardsiella." ScienceDaily. www.sciencedaily.com/releases/2013/12/131205092051.htm (accessed September 21, 2014).

Share This



More Plants & Animals News

Sunday, September 21, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: San Diego Zoo Welcomes Cheetah Cubs

Raw: San Diego Zoo Welcomes Cheetah Cubs

AP (Sep. 20, 2014) The San Diego Zoo has welcomed two Cheetah cubs to its Safari Park. The nearly three-week-old female cubs are being hand fed and are receiving around the clock care. (Sept. 20) Video provided by AP
Powered by NewsLook.com
Chocolate Museum Opens in Brussels

Chocolate Museum Opens in Brussels

AFP (Sep. 19, 2014) Considered a "national heritage" in Belgium, chocolate now has a new museum in Brussels. In a former chocolate factory, visitors to the permanent exhibition spaces, workshops and tastings can discover derivatives of the cocoa bean. Duration: 01:00 Video provided by AFP
Powered by NewsLook.com
Could Grief Affect The Immune Systems Of Senior Citizens?

Could Grief Affect The Immune Systems Of Senior Citizens?

Newsy (Sep. 19, 2014) The study found elderly people are much more likely to become susceptible to infection than younger adults going though a similar situation. Video provided by Newsy
Powered by NewsLook.com
Jury Delivers Verdict in Salmonella Trial

Jury Delivers Verdict in Salmonella Trial

AP (Sep. 19, 2014) A federal jury has convicted three people in connection with an outbreak of salmonella poisoning five years ago that sickened hundreds of people and was linked to a number of deaths. (Sept. 19) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins