Featured Research

from universities, journals, and other organizations

Largest developmental proteomic data set for any animal developed

Date:
March 27, 2014
Source:
University of Notre Dame
Summary:
Now that the human genome is sequenced, researchers are focusing on the study of the proteome, which is the protein content of an organism, tissue or cell. They have successfully tracked the changing patterns of protein expression during early development of Xenopus laevis, or African clawed frog, embryos. The largest data set on developmental proteomics for any organism has been developed by scientists, and have included the single-cell zygote.

Xenopus laevis, or African clawed frog.
Credit: Image courtesy of University of Notre Dame

Now that the human genome is sequenced, University of Notre Dame researchers are focusing on the study of the proteome, which is the protein content of an organism, tissue or cell. Bioanalytical chemist Norman Dovichi and molecular biologist Paul Huber have successfully tracked the changing patterns of protein expression during early development of Xenopus laevis, or African clawed frog, embryos. They have developed the largest data set on developmental proteomics for any organism, and have included the single-cell zygote.

Their research has uncovered an unexpected amount of discordance between the levels of messenger RNA (mRNA) and its corresponding protein. Their findings are published in Scientific Reports in an article titled, "Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development."

The Notre Dame team based in the Department of Chemistry and Biochemistry in the College of Science has identified and measured the levels of about 4,000 proteins, which exhibited patterns of expression that reflect key events during early Xenopus development. For example, the appearance of organ- and tissue-specific proteins, such as those found exclusively in cardiac muscle cells, accurately reflects imminent anatomical changes taking place in the embryo. The research could lead to insight into congenital birth defects that result from the misregulation of gene expression.

The research also contradicted a widely held assumption that the levels of mRNA, which encodes proteins, would be directly related to protein levels. While that was true in most cases, there were a surprisingly high number of exceptions, demonstrating that the amounts of a particular protein can be controlled by multiple mechanisms.

Because development takes place in well-defined stages outside the mother, Xenopus is a favored model. Embryogenesis can be easily monitored in real time; fate maps for organ development have been determined and major regulators of these processes have been identified and characterized, providing an abundance of tissue- and organ-specific markers to track embryo formation. Additionally, embryos develop rapidly, achieving a nearly fully developed nervous system within four days. "It's easy to manipulate the embryos to mimic certain disease states, making Xenopus extremely valuable to biologists," Huber said.

"The collaborative, ground-breaking work of Norm Dovichi, Paul Huber and their team is crucial to helping us understand the complexity of life. We are proud of this important milestone," said Greg Crawford, dean of the College of Science at the University of Notre Dame.


Story Source:

The above story is based on materials provided by University of Notre Dame. Note: Materials may be edited for content and length.


Journal Reference:

  1. Liangliang Sun, Michelle M. Bertke, Matthew M. Champion, Guijie Zhu, Paul W. Huber, Norman J. Dovichi. Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development. Scientific Reports, 2014; 4 DOI: 10.1038/srep04365

Cite This Page:

University of Notre Dame. "Largest developmental proteomic data set for any animal developed." ScienceDaily. ScienceDaily, 27 March 2014. <www.sciencedaily.com/releases/2014/03/140327170026.htm>.
University of Notre Dame. (2014, March 27). Largest developmental proteomic data set for any animal developed. ScienceDaily. Retrieved September 23, 2014 from www.sciencedaily.com/releases/2014/03/140327170026.htm
University of Notre Dame. "Largest developmental proteomic data set for any animal developed." ScienceDaily. www.sciencedaily.com/releases/2014/03/140327170026.htm (accessed September 23, 2014).

Share This



More Plants & Animals News

Tuesday, September 23, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Raw: Ice Age Wooly Mammoth Remains for Sale

Raw: Ice Age Wooly Mammoth Remains for Sale

AP (Sep. 23, 2014) A rare, well-preserved skeleton of a woolly mammoth is going on sale at Summers Place Auctions hope the 11.5-foot tall, almost intact specimen will fetch between $245,000 to $409,000. (Sept. 23) Video provided by AP
Powered by NewsLook.com
Fox Bites Conn. Student And School Staffers In Rare Attack

Fox Bites Conn. Student And School Staffers In Rare Attack

Newsy (Sep. 23, 2014) A fox attacked a second-grade boy at a Connecticut elementary school Monday. It also attacked two school staff members and a woman and her dog. Video provided by Newsy
Powered by NewsLook.com
Will Living Glue Be A Thing?

Will Living Glue Be A Thing?

Newsy (Sep. 23, 2014) Using proteins derived from mussels, engineers at MIT have made a supersticky underwater adhesive. They're now looking to make "living glue." Video provided by Newsy
Powered by NewsLook.com
Raw: Tiger Kills Man at India Zoo

Raw: Tiger Kills Man at India Zoo

AP (Sep. 23, 2014) A white tiger killed a young man who climbed over a fence at the New Delhi zoo and jumped into the animal's enclosure on Tuesday, a spokesman said. (Sept. 23) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins