Featured Research

from universities, journals, and other organizations

Resistance to antibiotics: New rapid diagnosis

Date:
June 26, 2014
Source:
Fribourg, Université de
Summary:
A rapid diagnostic test for multi-resistance to broad-spectrum antibiotics has just been developed by researchers. This new test allows the identification, in less than two hours, of multidrug-resistant strains of Acinetobacter baumannii, an important hospital pathogen. The large-scale application of this test will mean better control of the spread of certain traits of antibiotic resistance.

A rapid diagnostic test for multi-resistance to broad-spectrum antibiotics has just been developed at the University of Fribourg. Prof Patrice Nordmann and Dr Laurent Poirel of the Medical and Molecular Microbiology Unit have been collaborating with Unit 914 of the National Institute of Health and Medical Research (INSERM) in Paris, of which Patrice Nordmann is also Director. This new test allows the identification, in less than two hours, of multidrug-resistant strains of Acinetobacter baumannii, an important hospital pathogen. The large-scale application of this test will mean better control of the spread of certain traits of antibiotic resistance.

Related Articles


Bacterial resistance to antibiotics has increased considerably over recent years. The situation is particularly dramatic in regard to gram-negative bacilli (Escherichia coli and Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii), in, for example, septicemic and abdominal infections and infections of the urinary tract and the lungs, considered to be the most frequent human infections in 2014. There are already signs of a real therapeutic impasse. Extremely broad-spectrum antibiotics, such as the broad-spectrum cephalosporins and the carbapenems, antibiotics of last resort, are already proving totally ineffective against certain strains of bacteria. It is estimated that in Europe the total number of deaths associated with multi-resistance to antibiotics is 25,000 annually. The rapid development of this resistance risks compromising whole areas of 21st century medicine which require effective preventative or curative antibiotics for transplants and major surgery as well as resuscitation.

Rapid diagnosis: a crucial factor

When a bacterium hydrolyses an antibiotic, it deactivates the way it works in some way. It is this phenomenon which had already been targeted by two rapid diagnosis tests developed by Patrice Nordmann and Laurent Poirel. These tests detected the presence of extended spectrum Beta lactamase enzymes and of carbapenemases (which hydrolyse wide-spectrum cephalosporins and carbapenems in Enterobacteriaceae and Pseudomonas aeruginosa, respectively). Now the two researchers have developed the CarbAcineto NP test which allows the detection of carbapenemase activity in A. baumannii; and it is this carbapenemase activity which is systematically associated with multi-resistance to antibiotics in this type of bacterium (fig. 1). The test is based on the acidification properties generated by the enzymatic hydrolysis of a carbapenem, Imipenem, when it is cleaved by a carbapenemase. The medium acidifies and the acidity (pH) indicator then turns from red to yellow. The detection of this carbapenemase activity can be realised by testing already isolated bacteria or any site infections. The result is obtained in less than 2 hours, while other techniques currently available require a minimum of 24 hours, most frequently 72 hours. The sensitivity and specificity of the CarbAcineto NP test is close to 100%, a value rarely achieved by a diagnostic test in medicine.

The development of the CarbAcineto test is an important contribution to the struggle against the emergence of antibiotic resistance. It is simple, cost-effective and, by detecting multidrug-resistant strains, it can prevent them spreading via outbreaks of hospital infections caused by multidrug-resistant bacteria, particularly among the most seriously ill patients -- those undergoing resuscitation. This new test also provides a guide in the choice between the very few remaining treatment options for infected patients.


Story Source:

The above story is based on materials provided by Fribourg, Université de. Note: Materials may be edited for content and length.


Journal Reference:

  1. L. Dortet, L. Poirel, C. Errera, P. Nordmann. CarbAcineto NP test for rapid detection of carbapenemase-producers in Acinetobacter spp.. Journal of Clinical Microbiology, 2014; DOI: 10.1128/JCM.00594-14

Cite This Page:

Fribourg, Université de. "Resistance to antibiotics: New rapid diagnosis." ScienceDaily. ScienceDaily, 26 June 2014. <www.sciencedaily.com/releases/2014/06/140626095705.htm>.
Fribourg, Université de. (2014, June 26). Resistance to antibiotics: New rapid diagnosis. ScienceDaily. Retrieved November 28, 2014 from www.sciencedaily.com/releases/2014/06/140626095705.htm
Fribourg, Université de. "Resistance to antibiotics: New rapid diagnosis." ScienceDaily. www.sciencedaily.com/releases/2014/06/140626095705.htm (accessed November 28, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Friday, November 28, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Research on Bats Could Help Develop Drugs Against Ebola

Research on Bats Could Help Develop Drugs Against Ebola

AFP (Nov. 28, 2014) — In Africa's only biosafety level 4 laboratory, scientists have been carrying out experiments on bats to understand how virus like Ebola are being transmitted, and how some of them resist to it. Duration: 01:18 Video provided by AFP
Powered by NewsLook.com
New Dinosaur Species Found in Museum Collection

New Dinosaur Species Found in Museum Collection

Reuters - Innovations Video Online (Nov. 27, 2014) — A British palaeontologist has discovered a new species of dinosaur while studying fossils in a Canadian museum. Pentaceratops aquilonius was related to Triceratops and lived at the end of the Cretaceous Period, around 75 million years ago. Jim Drury has more. Video provided by Reuters
Powered by NewsLook.com
Tryptophan Isn't Making You Sleepy On Thanksgiving

Tryptophan Isn't Making You Sleepy On Thanksgiving

Newsy (Nov. 27, 2014) — Tryptophan, a chemical found naturally in turkey meat, gets blamed for sleepiness after Thanksgiving meals. But science points to other culprits. Video provided by Newsy
Powered by NewsLook.com
Classic Hollywood Memorabilia Goes Under the Hammer

Classic Hollywood Memorabilia Goes Under the Hammer

Reuters - Entertainment Video Online (Nov. 26, 2014) — The iconic piano from "Casablanca" and the Cowardly Lion suit from "The Wizard of Oz" fetch millions at auction. Sara Hemrajani reports. Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins