Featured Research

from universities, journals, and other organizations

Resistance to antibiotics: New rapid diagnosis

Date:
June 26, 2014
Source:
Fribourg, Université de
Summary:
A rapid diagnostic test for multi-resistance to broad-spectrum antibiotics has just been developed by researchers. This new test allows the identification, in less than two hours, of multidrug-resistant strains of Acinetobacter baumannii, an important hospital pathogen. The large-scale application of this test will mean better control of the spread of certain traits of antibiotic resistance.

A rapid diagnostic test for multi-resistance to broad-spectrum antibiotics has just been developed at the University of Fribourg. Prof Patrice Nordmann and Dr Laurent Poirel of the Medical and Molecular Microbiology Unit have been collaborating with Unit 914 of the National Institute of Health and Medical Research (INSERM) in Paris, of which Patrice Nordmann is also Director. This new test allows the identification, in less than two hours, of multidrug-resistant strains of Acinetobacter baumannii, an important hospital pathogen. The large-scale application of this test will mean better control of the spread of certain traits of antibiotic resistance.

Bacterial resistance to antibiotics has increased considerably over recent years. The situation is particularly dramatic in regard to gram-negative bacilli (Escherichia coli and Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii), in, for example, septicemic and abdominal infections and infections of the urinary tract and the lungs, considered to be the most frequent human infections in 2014. There are already signs of a real therapeutic impasse. Extremely broad-spectrum antibiotics, such as the broad-spectrum cephalosporins and the carbapenems, antibiotics of last resort, are already proving totally ineffective against certain strains of bacteria. It is estimated that in Europe the total number of deaths associated with multi-resistance to antibiotics is 25,000 annually. The rapid development of this resistance risks compromising whole areas of 21st century medicine which require effective preventative or curative antibiotics for transplants and major surgery as well as resuscitation.

Rapid diagnosis: a crucial factor

When a bacterium hydrolyses an antibiotic, it deactivates the way it works in some way. It is this phenomenon which had already been targeted by two rapid diagnosis tests developed by Patrice Nordmann and Laurent Poirel. These tests detected the presence of extended spectrum Beta lactamase enzymes and of carbapenemases (which hydrolyse wide-spectrum cephalosporins and carbapenems in Enterobacteriaceae and Pseudomonas aeruginosa, respectively). Now the two researchers have developed the CarbAcineto NP test which allows the detection of carbapenemase activity in A. baumannii; and it is this carbapenemase activity which is systematically associated with multi-resistance to antibiotics in this type of bacterium (fig. 1). The test is based on the acidification properties generated by the enzymatic hydrolysis of a carbapenem, Imipenem, when it is cleaved by a carbapenemase. The medium acidifies and the acidity (pH) indicator then turns from red to yellow. The detection of this carbapenemase activity can be realised by testing already isolated bacteria or any site infections. The result is obtained in less than 2 hours, while other techniques currently available require a minimum of 24 hours, most frequently 72 hours. The sensitivity and specificity of the CarbAcineto NP test is close to 100%, a value rarely achieved by a diagnostic test in medicine.

The development of the CarbAcineto test is an important contribution to the struggle against the emergence of antibiotic resistance. It is simple, cost-effective and, by detecting multidrug-resistant strains, it can prevent them spreading via outbreaks of hospital infections caused by multidrug-resistant bacteria, particularly among the most seriously ill patients -- those undergoing resuscitation. This new test also provides a guide in the choice between the very few remaining treatment options for infected patients.


Story Source:

The above story is based on materials provided by Fribourg, Université de. Note: Materials may be edited for content and length.


Journal Reference:

  1. L. Dortet, L. Poirel, C. Errera, P. Nordmann. CarbAcineto NP test for rapid detection of carbapenemase-producers in Acinetobacter spp.. Journal of Clinical Microbiology, 2014; DOI: 10.1128/JCM.00594-14

Cite This Page:

Fribourg, Université de. "Resistance to antibiotics: New rapid diagnosis." ScienceDaily. ScienceDaily, 26 June 2014. <www.sciencedaily.com/releases/2014/06/140626095705.htm>.
Fribourg, Université de. (2014, June 26). Resistance to antibiotics: New rapid diagnosis. ScienceDaily. Retrieved September 29, 2014 from www.sciencedaily.com/releases/2014/06/140626095705.htm
Fribourg, Université de. "Resistance to antibiotics: New rapid diagnosis." ScienceDaily. www.sciencedaily.com/releases/2014/06/140626095705.htm (accessed September 29, 2014).

Share This



More Plants & Animals News

Monday, September 29, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

California University Designs Sustainable Winery

California University Designs Sustainable Winery

Reuters - US Online Video (Sep. 27, 2014) — Amid California's worst drought in decades, scientists at UC Davis design a sustainable winery that includes a water recycling system. Vanessa Johnston reports. Video provided by Reuters
Powered by NewsLook.com
Argentina Worries Over Decline of Soybean Prices

Argentina Worries Over Decline of Soybean Prices

AFP (Sep. 27, 2014) — The drop in price of soy on the international market is a cause for concern in Argentina, as soybean exports are a major source of income for Latin America's third largest economy. Duration: 01:10 Video provided by AFP
Powered by NewsLook.com
Mama Bear, Cubs Hang out in California Backyard

Mama Bear, Cubs Hang out in California Backyard

Reuters - US Online Video (Sep. 27, 2014) — A mama bear and her two cubs climb trees, wrestle and take naps in the backyard of a Monrovia, California home. Vanessa Johnston reports. Video provided by Reuters
Powered by NewsLook.com
'Crazy' Climate Forces Colombian Farmers to Adapt

'Crazy' Climate Forces Colombian Farmers to Adapt

AFP (Sep. 26, 2014) — Once upon a time, farming was a blissfully low-tech business on Colombia's northern plains. Duration: 02:05 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:

Strange & Offbeat Stories

 

Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins