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Zeroing in on a silent killer

Date:
April 23, 2015
Source:
University of Southern California
Summary:
Researchers have solved the molecular structure of an important regulator for blood pressure in the human body -- which could lead to better treatments for high blood pressure.
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This is an illustration of the effects of angiotensin on the AT1R (vasoconstriction, high blood pressure) compared to blocking the receptor (relaxed blood vessel, normal blood pressure).
Credit: Courtesy of Katya Kadyshevskaya / Bridge Institute at USC

One in three Americans has high blood pressure, a long-term constriction of arteries that can lead to coronary heart disease, heart failure and stroke.

Using a sophisticated X-ray analysis, a US-German team of scientists revealed the molecular structure of the angiotensin receptor AT1R, an important regulator for blood pressure in the human body.

Their work could fast-track the development of new medications with fewer side-effects, according to Vadim Cherezov, lead researcher and professor at the USC Dornsife College of Letters, Arts and Sciences.

Angiotensin receptor AT1R, when activated by the hormone angiotensin, triggers two major signaling pathways inside of cells. One of them, mediated by G proteins (a family of proteins that act as switches and transmit signals through cell walls), causes the constriction of blood vessels -- leading to an increase in blood pressure. Another pathway, mediated by arrestin, confers a number of beneficial effects.

Doctors regularly prescribe drugs, known as angiotensin receptor blockers, that turn off both pathways, which prevents the constriction but also has side effects, such as dizziness, headache, drowsiness, and elevated levels of potassium in the blood.

"It's like using a two-by-four to kill a fly. Yes, it works -- but perhaps a more refined approach could achieve the positive results without many side effects by only blocking the G protein pathway, while keeping the arresting pathway active," Cherezov said. "To do so, you need to understand exactly how and where drug-like molecules bind to the receptor and what conformational changes they produce."

The researchers created crystals of the receptor in complex with an angiotensin receptor blocker. Then, they used the world's most powerful X-ray laser to zap the crystals with flashes of energy strong enough to produce diffraction patterns.

By interpreting those patterns, the scientists were able to piece together the receptor's structure with a resolution of 0.29 nanometres -- an atomic scale, showing precisely where the drug molecule is bound.

"Despite its medical relevance, the structure of this receptor was unknown up to now," said study co-author Cornelius Gati, of the Center for Free-Electron Laser Science at Deutsches Elektronen-Synchrotron (DESY) in Germany.

"The data show the exact structure of the binding pocket and the interaction with blood pressure drugs binding there. This provides new insights into the mode of action and facilitates the development of new drugs," he said.

Their findings appear on April 23 in the journal Cell. The scientists said they hope that the exact knowledge of the molecular receptor structure can help to design tailor-made blood pressure drugs with fewer side effects.

"Our work represents a first step in this direction," says Cherezov, who next plans to continue study on this receptor and another, closely related receptor.


Story Source:

Materials provided by University of Southern California. Original written by Robert Perkins. Note: Content may be edited for style and length.


Journal Reference:

  1. Haitao Zhang, Hamiyet Unal, Cornelius Gati, Gye Won Han, Wei Liu, Nadia A. Zatsepin, Daniel James, Dingjie Wang, Garrett Nelson, Uwe Weierstall, Michael R. Sawaya, Qingping Xu, Marc Messerschmidt, Garth J. Williams, Sébastien Boutet, Oleksandr M. Yefanov, Thomas A. White, Chong Wang, Andrii Ishchenko, Kalyan C. Tirupula, Russell Desnoyer, Jesse Coe, Chelsie E. Conrad, Petra Fromme, Raymond C. Stevens, Vsevolod Katritch, Sadashiva S. Karnik, Vadim Cherezov. Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography. Cell, 2015; DOI: 10.1016/j.cell.2015.04.011

Cite This Page:

University of Southern California. "Zeroing in on a silent killer." ScienceDaily. ScienceDaily, 23 April 2015. <www.sciencedaily.com/releases/2015/04/150423143241.htm>.
University of Southern California. (2015, April 23). Zeroing in on a silent killer. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2015/04/150423143241.htm
University of Southern California. "Zeroing in on a silent killer." ScienceDaily. www.sciencedaily.com/releases/2015/04/150423143241.htm (accessed May 23, 2017).

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