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Two-color X-rays give scientists 3-D view of the unknown

September 2, 2015
Department of Energy, Office of Science
Scientists can now get a high-resolution view of a sample or the details of the first steps in ultra-fast processes, thanks to new research.

The top figure shows the energy/time distribution of the twin bunches measured with an X-band transverse deflector. The horizontal dimension represents time and the vertical represents energy. The bottom figure shows the measured temporal profile of the two X-ray pulses.
Credit: Image courtesy of SLAC National Accelerator Laboratory

A high-resolution view of a sample or details of the first steps in ultra-fast processes are now available, thanks to researchers at SLAC National Accelerator Laboratory's Linac Coherent Light Source (LCLS). They incorporated a series of advances to hit samples with a pair of precisely tuned X-ray laser pulses of different colors, or photon energies. The two pulses can arrive at the same time providing a detailed three-dimensional view of the sample's structure. Delaying the beams by tens of femtoseconds (quadrillionths of a second) allows scientists to study very fast processes, such as the earliest steps of a chemical reaction.

For the first time, X-ray scientists have access and independent control of ultra-short two-color X-ray pulses with nearly the full peak power of the LCLS (above 50 GW). This new capability represents an improvement by an order of magnitude over what was possible before making it easier and faster for scientists to better dissect hard-to-study chemical and material science samples as well as map medically important proteins, such as signaling proteins of interest as pharmaceutical targets.

At the LCLS, researchers have demonstrated the generation of two-color X-ray pulses using twin electron bunches. In this technique, two electron bunches are generated by illuminating the injector photocathode with a train of two laser pulses separated in time by a few picoseconds. After being accelerated and compressed, the two electron bunches are spaced by tens of femtoseconds and have two distinct energies. When the high brightness bunched electron beams are delivered to a sequence of alternating magnets called an undulator, the two bunches emit two independent X-ray pulses of different photon energies.

The energy difference and the arrival time delay of the two pulses are controllable independently, which enables the study of dynamical processes excited by X-rays at the femtosecond time scale. The existing hard X-ray self-seeding system can be employed to simultaneously filter the two pulses generated by the twin bunches, allowing the generation of two narrow-bandwidth X-ray laser pulses with a wide energy separation. This enables the 3-D structural determination of previously unmapped proteins using femtosecond serial crystallography at LCLS.

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Journal References:

  1. A. Marinelli, D. Ratner, A.A. Lutman, J. Turner, J. Welch, F.-J. Decker, H. Loos, C. Behrens, S. Gilevich, A.A. Miahnahri, S. Vetter, T.J. Maxwell, Y. Ding, R. Coffee, S. Wakatsuki, Z. Huang. High-intensity double-pulse X-ray free-electron laser. Nature Communications, 2015; 6: 6369 DOI: 10.1038/ncomms7369
  2. A. A. Lutman, F.-J Decker, J. Arthur, M. Chollet, Y. Feng, J. Hastings, Z. Huang, H. Lemke, H.-D. Nuhn, A. Marinelli, J. L. Turner, S. Wakatsuki, J. Welch, D. Zhu. Demonstration of Single-Crystal Self-Seeded Two-Color X-Ray Free-Electron Lasers. Physical Review Letters, 2014; 113 (25) DOI: 10.1103/PhysRevLett.113.254801
  3. Zhen Zhang, Yuantao Ding, Agostino Marinelli, Zhirong Huang. Longitudinal dynamics of twin electron bunches in the Linac Coherent Light Source. Physical Review Special Topics - Accelerators and Beams, 2015; 18 (3) DOI: 10.1103/PhysRevSTAB.18.030702

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Department of Energy, Office of Science. "Two-color X-rays give scientists 3-D view of the unknown." ScienceDaily. ScienceDaily, 2 September 2015. <>.
Department of Energy, Office of Science. (2015, September 2). Two-color X-rays give scientists 3-D view of the unknown. ScienceDaily. Retrieved May 23, 2017 from
Department of Energy, Office of Science. "Two-color X-rays give scientists 3-D view of the unknown." ScienceDaily. (accessed May 23, 2017).