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Skin cells 'crawl' together to heal wounds treated with unique hydrogel layer

Research team led by Milica Radisic uses their patented peptide to close non-healing chronic wounds caused by diabetes

Date:
December 14, 2016
Source:
University of Toronto Faculty of Applied Science & Engineering
Summary:
Scientist have demonstrated for the first time that their peptide-hydrogel biomaterial prompts skin cells to 'crawl' toward one another, closing chronic, non-healing wounds often associated with diabetes, such as bed sores and foot ulcers.
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U of T Engineering researchers Dr. Lewis Reis (at left) and Professor Milica Radisic used their unique peptide-hydrogel biomaterial to close non-healing wounds twice as quickly as commercially available products.
Credit: Marit Mitchell / U of T Engineering

Time may not heal all wounds, but a proprietary mix of peptides and gel developed by U of T Engineering researchers heals most.

A team led by Professor Milica Radisic has demonstrated for the first time that their peptide-hydrogel biomaterial prompts skin cells to "crawl" toward one another, closing chronic, non-healing wounds often associated with diabetes, such as bed sores and foot ulcers.

The team tested their biomaterial on healthy cells from the surface of human skin, called keratinocytes, as well as on keratinocytes derived from elderly diabetic patients. They saw non-healing wounds close 200 per cent faster than with no treatment, and 60 per cent faster than treatment with a leading commercially used collagen-based product.

"We were happy when we saw the cells crawl together much faster with our biomatieral, but if it didn't work with diabetic cells, that would have been the end of the story," says Radisic. "But even the diabetic cells travelled much faster -- that's huge."

Until now, most treatments for chronic wounds involved applying topical ointments that promote the growth of blood vessels to the area. But in diabetic patients, blood vessel growth is inhibited, making those treatments ineffective. Radisic and her team have been working with their special peptide -- called QHREDGS, or Q-peptide for short -- for almost 10 years. They knew it promoted survival of many different cell types, including stem cells, heart cells and fibroblasts (the cells that make connective tissues), but had never applied it to wound healing.

"We thought that if we were able to use our peptide to both promote survival and give these skin cells a substrate so they could crawl together, they would be able to close the wound more quickly," says Radisic. "That was the underlying hypothesis."

Radisic and PhD students Yun Xiao and Lewis Reis compared the Q-peptide-hydrogel mix to the commercially available collagen dressing, to hydrogels without the peptide, and to no treatment. They found that a single dose of their peptide-hydrogel biomaterial closed the wounds in less than two weeks. Their work was published in the journal Proceedings of the National Academy of Sciences.

"Currently, there are therapies for diabetic foot ulcers, but they can be improved," says Xiao, the paper's lead author. "Diabetic wound healing is a complicated condition, because many aspects of the normal wound healing process are disrupted -- I know people with diabetic foot ulcers, and the possibility to improve their lives drove me throughout this work."

The multidisciplinary team worked with Covalon Technologies Ltd., a company dedicated to the research and development and commercialization of novel healthcare technologies, on this project. Covalon's chief scientific officer, Dr. Val DiTizio, has been leading the partnership with Radisic's group for about three years, and contributed its collagen-based wound-healing dressing, ColActive, as one of the controls.

"We believe strongly in keeping abreast of new technologies being developed in academia," says DiTizio, who is also working with Radisic on a bone-regeneration project. "Collaborations such as this one inform our future research directions and help make our products better."

This finding could have big implications for many types of wound treatments, from recovery after a heart attack to healing post-surgery. Accelerated healing times also introduces the added benefit of reducing the opportunity for infection, says Reis.

"One of the biggest challenges with the work was convincing our peers that the results we were getting were indeed true, as they were staggering, even to us," he says. "Being confident in our methods and diligent in our research and analysis prevailed in the end."


Story Source:

Materials provided by University of Toronto Faculty of Applied Science & Engineering. Original written by Marit Mitchell. Note: Content may be edited for style and length.


Journal Reference:

  1. Yun Xiao, Lewis A. Reis, Nicole Feric, Erica J. Knee, Junhao Gu, Shuwen Cao, Carol Laschinger, Camila Londono, Julia Antolovich, Alison P. McGuigan, Milica Radisic. Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization. Proceedings of the National Academy of Sciences, 2016; 113 (40): E5792 DOI: 10.1073/pnas.1612277113

Cite This Page:

University of Toronto Faculty of Applied Science & Engineering. "Skin cells 'crawl' together to heal wounds treated with unique hydrogel layer: Research team led by Milica Radisic uses their patented peptide to close non-healing chronic wounds caused by diabetes." ScienceDaily. ScienceDaily, 14 December 2016. <www.sciencedaily.com/releases/2016/12/161214090315.htm>.
University of Toronto Faculty of Applied Science & Engineering. (2016, December 14). Skin cells 'crawl' together to heal wounds treated with unique hydrogel layer: Research team led by Milica Radisic uses their patented peptide to close non-healing chronic wounds caused by diabetes. ScienceDaily. Retrieved May 29, 2017 from www.sciencedaily.com/releases/2016/12/161214090315.htm
University of Toronto Faculty of Applied Science & Engineering. "Skin cells 'crawl' together to heal wounds treated with unique hydrogel layer: Research team led by Milica Radisic uses their patented peptide to close non-healing chronic wounds caused by diabetes." ScienceDaily. www.sciencedaily.com/releases/2016/12/161214090315.htm (accessed May 29, 2017).

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