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New ultrasound technique is first to image inside live cells

Date:
December 21, 2016
Source:
University of Nottingham
Summary:
Researchers have developed a breakthrough technique that uses sound rather than light to see inside live cells, with potential application in stem-cell transplants and cancer diagnosis.
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These are stem cells becoming fat cells. Using the ultrasound technique, we can start exploring why fat cells put on fat and whether we can make the cells 'diet'.
Credit: University of Nottingham

Researchers at The University of Nottingham have developed a break-through technique that uses sound rather than light to see inside live cells, with potential application in stem-cell transplants and cancer diagnosis.

The new nanoscale ultrasound technique uses shorter-than-optical wavelengths of sound and could even rival the optical super-resolution techniques which won the 2014 Nobel Prize for Chemistry.

This new kind of sub-optical phonon (sound) imaging provides invaluable information about the structure, mechanical properties and behaviour of individual living cells at a scale not achieved before.

Researchers from the Optics and Photonics group in the Faculty of Engineering, University of Nottingham, are behind the discovery, which is published in the paper 'High resolution 3D imaging of living cells with sub-optical wavelength phonons' in the journal, Scientific Reports.

"People are most familiar with ultrasound as a way of looking inside the body -- in the simplest terms we've engineered it to the point where it can look inside an individual cell. Nottingham is currently the only place in the world with this capability," said Professor Matt Clark, who contributed to the study.

In conventional optical microscopy, which uses light (photons), the size of the smallest object you can see (or the resolution) is limited by the wavelength.

For biological specimens, the wavelength cannot go smaller than that of blue light because the energy carried on photons of light in the ultraviolet (and shorter wavelengths) is so high it can destroy the bonds that hold biological molecules together damaging the cells.

Optical super-resolution imaging also has distinct limitations in biological studies. This is because the fluorescent dyes it uses are often toxic and it requires huge amounts of light and time to observe and reconstruct an image which is damaging to cells.

Unlike light, sound does not have a high-energy payload. This has enabled the Nottingham researchers to use smaller wavelengths and see smaller things and get to higher resolutions without damaging the cell biology.

"A great thing is that, like ultrasound on the body, ultrasound in the cells causes no damage and requires no toxic chemicals to work. Because of this we can see inside cells that one day might be put back into the body, for instance as stem-cell transplants," adds Professor Clark.


Story Source:

Materials provided by University of Nottingham. Note: Content may be edited for style and length.


Journal Reference:

  1. Fernando Pérez-Cota, Richard J. Smith, Emilia Moradi, Leonel Marques, Kevin F. Webb, Matt Clark. High resolution 3D imaging of living cells with sub-optical wavelength phonons. Scientific Reports, 2016; 6: 39326 DOI: 10.1038/srep39326

Cite This Page:

University of Nottingham. "New ultrasound technique is first to image inside live cells." ScienceDaily. ScienceDaily, 21 December 2016. <www.sciencedaily.com/releases/2016/12/161221111327.htm>.
University of Nottingham. (2016, December 21). New ultrasound technique is first to image inside live cells. ScienceDaily. Retrieved May 25, 2017 from www.sciencedaily.com/releases/2016/12/161221111327.htm
University of Nottingham. "New ultrasound technique is first to image inside live cells." ScienceDaily. www.sciencedaily.com/releases/2016/12/161221111327.htm (accessed May 25, 2017).

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