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You say genome editing, I say natural mutation

October 19, 2023
Cold Spring Harbor Laboratory
A plant geneticist and computational biologist teamed up to decipher the unpredictability of natural and engineered mutations in tomatoes. They discovered some combinations of mutations behave as expected while others are more erratic. Their work may help scientists find some order in the chaos of evolution and genome editing.

For tens of thousands of years, evolution shaped tomatoes through natural mutations. Then, humans came along. For centuries, we've bred and cherry-picked tomatoes with our preferred traits. Today, CRISPR genome editing allows us to make new crop mutations that improve traits even further. However, individual mutations, whether natural or engineered, don't work alone. Each operates in a sea of thousands of so-called "background" mutations. These changes have been sowed by evolution and agricultural history. And what if just one could dramatically alter the desired outcome of an engineered mutation?

Now, a plant geneticist and a computational scientist at Cold Spring Harbor Laboratory (CSHL) have teamed up to explore just how predictable plant breeding actually is with natural and CRISPR mutations. To do so, they turned back the evolutionary clock.

CSHL Professor & HHMI Investigator Zachary Lippman and Associate Professor David McCandlish wondered if different natural and engineered mutations could have similar effects on tomato size depending on the presence of two other gene mutations. Using CRISPR, they created a series of mutations in the SlCLV3 gene. (Natural mutation of this gene is known to increase fruit size.) They then combined those mutations with others in genes that work with SlCLV3.

Altogether, they created 46 tomato strains with different combinations of mutations. They found the SlCLV3 mutations produced more predictable effects when certain other mutations were also present. Mutations in one gene produced predictable changes in tomato size, but mutations in another yielded random outcomes. Remarkably, the most beneficial effect involved two mutations that arose millennia ago and were central in tomato domestication.

New research by McCandlish and Lippman may help us better understand genetic predictability. But one thing's certain. Context matters when introducing new crop mutations. Lippman explains:

"Is genome editing a way to quickly bring in consumer benefits -- better flavor, nutrition? The answer is probably yes. The question is how predictable is it going to be."

Lippman and McCandlish's work suggests the role of background mutations demands reassessment. "The field will have to grapple with this as we start to make more highly engineered organisms," says McCandlish. "Once you start making 10, 20 mutations, the probability of having unanticipated results may increase."

The book of evolution has been written in all different languages, many of which we're still learning. Plant genetics and computational biology offer two means of deciphering the text. Lippman and McCandlish hope their collaborative interpretation will help science meet the challenge. Looking ahead, it may also help humanity adapt crops to meet the ever-evolving needs of society.

Story Source:

Materials provided by Cold Spring Harbor Laboratory. Original written by Luis Sandoval. Note: Content may be edited for style and length.

Journal Reference:

  1. Lyndsey Aguirre, Anat Hendelman, Samuel F. Hutton, David M. McCandlish, Zachary B. Lippman. Idiosyncratic and dose-dependent epistasis drives variation in tomato fruit size. Science, 2023; 382 (6668): 315 DOI: 10.1126/science.adi5222

Cite This Page:

Cold Spring Harbor Laboratory. "You say genome editing, I say natural mutation." ScienceDaily. ScienceDaily, 19 October 2023. <>.
Cold Spring Harbor Laboratory. (2023, October 19). You say genome editing, I say natural mutation. ScienceDaily. Retrieved April 16, 2024 from
Cold Spring Harbor Laboratory. "You say genome editing, I say natural mutation." ScienceDaily. (accessed April 16, 2024).

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