New! Sign up for our free email newsletter.
Science News
from research organizations

Team creates novel rabies viral vectors for neural circuit mapping

New tools can detect microstructural changes in aging and Alzheimer's disease brain neurons

Date:
February 14, 2024
Source:
University of California - Irvine
Summary:
A research team has created 20 new recombinant rabies viral vectors for neural circuit mapping that offer a range of significant advantages over existing tools, including the ability to detect microstructural changes in models of aging and Alzheimer's disease brain neurons.
Share:
FULL STORY

A research team led by the University of California, Irvine has created 20 new recombinant rabies viral vectors for neural circuit mapping that offer a range of significant advantages over existing tools, including the ability to detect microstructural changes in models of aging and Alzheimer's disease brain neurons.

The study published today online in the journal Molecular Psychiatry, introduced proof-of-concept data demonstrating the power of these new vectors, which express a range of improved fluorescent proteins to provide expanded multi-scale multi-modal capabilities. Naturally occurring rabies infections target the nervous system. Scientists harnessed this tendency to create engineered forms of the rabies virus that are coupled to sensors and other payloads -- for example, some respond to light by turning bright green and act as tracers that map brain circuits.

"Viral genetic tools are critical for improving anatomical mapping and functional studies of cell-type-specific and circuit-specific neural networks," said Xiangmin Xu, co-corresponding author and UCI Chancellor's Professor of anatomy & neurobiology and director of the Center for Neural Circuit Mapping. "These new variants significantly enhance the capability and reach of neural labeling and circuit mapping across microscopic and macroscopic imaging scales and modalities, including 3D light and X-ray microscopy. We will make these new tools readily available to the neuroscience community through our established service platform at the CNCM."

These new recombinant viral vectors are designed to target very specific components of neuron biology in order to analyze pathological changes that occur during Alzheimer's disease and other brain diseases. They can be targeted to specific sub-cellular locations and organelles, as well as live imaging of neuronal activities using calcium indicators. The team conducted imaging analysis of mouse brains to demonstrate the discovery power of these new tools.

"These cutting-edge tools hold immense potential for understanding neural circuitry in both normal and pathological conditions and offer the ability to target specific regions of the brain with precision peptides or proteins to modulate neuronal functions for targeted treatment strategies," said Bert Semler, co-corresponding author and UCI Distinguished Professor of Microbiology & Molecular Genetics.

Alexis Bouin, Ph.D. and Ginny Wu are co-first authors who led and coordinated the project. Additional team members include Orkide Koyuncu, assistant professor of microbiology & molecular genetics; Qiao Ye, graduate student; Michele Wu, Liqi Tong, Ph.D., and Lujia Chen, Ph.D., members of the Xu lab, and Todd Holmes, professor of physiology & biophysics. Leveraging the UCI Center for Neural Circuit Mapping's broad collaborative network, team members from UCSD include Keun-Young Kim, Sebastien Phan, Mason R. Mackey, Ranjan Ramachandra,and Distinguished Professor Mark H. Ellisman.

This work was supported by National Institutes of Health grants RF1MH120020, R01FD007478, R35GM127102, R24GM137200, U24NS120055, and R01DA038896; and National Science Foundation grant NSF2014865-UTA20-00890.



Story Source:

Materials provided by University of California - Irvine. Note: Content may be edited for style and length.


Journal Reference:

  1. Alexis Bouin, Ginny Wu, Orkide O. Koyuncu, Qiao Ye, Keun-Young Kim, Michele Y. Wu, Liqi Tong, Lujia Chen, Sebastien Phan, Mason R. Mackey, Ranjan Ramachandra, Mark H. Ellisman, Todd C. Holmes, Bert L. Semler, Xiangmin Xu. New rabies viral resources for multi-scale neural circuit mapping. Molecular Psychiatry, 2024; DOI: 10.1038/s41380-024-02451-6

Cite This Page:

University of California - Irvine. "Team creates novel rabies viral vectors for neural circuit mapping." ScienceDaily. ScienceDaily, 14 February 2024. <www.sciencedaily.com/releases/2024/02/240214150309.htm>.
University of California - Irvine. (2024, February 14). Team creates novel rabies viral vectors for neural circuit mapping. ScienceDaily. Retrieved April 19, 2024 from www.sciencedaily.com/releases/2024/02/240214150309.htm
University of California - Irvine. "Team creates novel rabies viral vectors for neural circuit mapping." ScienceDaily. www.sciencedaily.com/releases/2024/02/240214150309.htm (accessed April 19, 2024).

Explore More

from ScienceDaily

RELATED STORIES