Featured Research

from universities, journals, and other organizations

A 'Repulsive' Protein Guides Blood Vessel Development

Date:
November 25, 2004
Source:
Howard Hughes Medical Institute
Summary:
In a developing embryo, the growth of nerves cannot outpace the establishment of life-giving blood vessels. Now, researchers have found that a protein intimately involved in blood vessel patterning actually belongs to a family of proteins known to guide neural development.

The image shows a low magnification picture of the mouse vasculature. The vessels along the trunk region are patterned by the Sema3E-plexin-D1 signaling module.
Credit: Photo : Laboratory of David Ginty/HHMI at The Johns Hopkins University School of Medicine

In a developing embryo, the growth of nerves cannot outpace the establishment of life-giving blood vessels. Now, researchers have found that a protein intimately involved in blood vessel patterning actually belongs to a family of proteins known to guide neural development.

The researchers said the studies provide more evidence of communication between developing nerves and blood vessels. Understanding how those networks talk to each other could help researchers devise methods to prevent blood vessel growth in tumors selectively - an approach to cancer treatment known as anti-angiogenesis.

The research team, which included Howard Hughes Medical Institute investigators David D. Ginty and Thomas M. Jessell, published its findings November 18, 2004, in Science Express, the early online version of the journal Science. Co-first authors of the paper were Chenghua Gu in Ginty's laboratory at The Johns Hopkins University School of Medicine, and Yutaka Yoshida in Jessell's laboratory at Columbia University.

In their experiments, the researchers explored the roles of two proteins involved in vascular development. One of the molecules, Semaphorin 3E (Sema3E), is a member of a family of protein signals that guides the growth of nerve cells. The other protein, plexin-D1, is a receptor protein that nestles in the membranes of growing cells and responds to external signaling proteins.

Ginty said that before the current study, plexin-D1 was known to be important for vascular development, but the specific signal to which it responded was a mystery. The molecule was also considered an important receptor in nerve cell development, and for that reason Jessell's laboratory was actively investigating plexin-D1.

Studies by Ginty and others, including former HHMI investigator Marc Tessier-Lavigne, who is now at Genentech, had shown that some of the semaphorins bind to a receptor called neuropilin, which is critical for vascular patterning in the embryo. However, in their earlier work, Gu, Ginty, and co-author Alex Kolodkin showed that semaphorins do not need to bind to neuropilin for normal patterning to occur.

“That work set us looking for other potential mechanisms by which semaphorins might control vascular pattern development,” said Ginty. The researchers found Sema3E in regions of the developing embryo that suggested that it should have a role in the patterning of blood vessels. They also found a strikingly similar pattern of expression of the blood vessel cell receptor plexin-D1, leading the researchers to hypothesize that Sema3E might be the signaling molecule that interacts with plexin-D1. If this were true, it suggested that Sema3E exerts a “repulsive” force, channeling the blood vessels to grow along their proper course.

Meanwhile, Yoshida discovered that unlike other members of the same protein family, Sema3E binds selectively to plexin-D1 - a strong hint that the two signals work together to control vascular patterning. Yoshida also found that Sema3E can bind to plexin-D1 whether or not it binds to neuropilin.

Researchers noted that in contrast to the careful patterning of blood vessels in normal mice, the pattern of blood vessels in mice lacking plexin-D1, produced in Jessell's laboratory, was haphazard. Furthermore, knockout mice lacking Sema3E, produced in the laboratory of co-author Christopher Henderson of the Developmental Biology Institute in France, showed the same defective patterning.

In additional experiments, Gu showed that overexpression of Sema3E protein in specific regions of chick embryos prevented vascular growth into those areas.

“Sema3E is a very potent chemorepellent for developing blood vessels,” Ginty noted. “So, one possibility is that drugs that mimic this function could be useful in preventing growth of the new blood vessels required by tumors.”

“One of the really interesting things about this paper is that it questions the idea that Sema3E's binding to neuropilin is required for vascular patterning,” said Jessell. “This, together with the finding that Sema3E interacts with plexin-D1, independent of neuropilin, may turn some of the preconceptions about the role of neuropilins in vascular patterning on their head.”

Jessell and his colleagues are now exploring whether Sema3E and plexin-D1 also contribute to the development of connections in the spinal cord. Ginty and his colleagues plan to explore the role of the proteins in neural development, as well as whether the combination is involved in vascular patterning in the limbs.


Story Source:

The above story is based on materials provided by Howard Hughes Medical Institute. Note: Materials may be edited for content and length.


Cite This Page:

Howard Hughes Medical Institute. "A 'Repulsive' Protein Guides Blood Vessel Development." ScienceDaily. ScienceDaily, 25 November 2004. <www.sciencedaily.com/releases/2004/11/041123103713.htm>.
Howard Hughes Medical Institute. (2004, November 25). A 'Repulsive' Protein Guides Blood Vessel Development. ScienceDaily. Retrieved September 22, 2014 from www.sciencedaily.com/releases/2004/11/041123103713.htm
Howard Hughes Medical Institute. "A 'Repulsive' Protein Guides Blood Vessel Development." ScienceDaily. www.sciencedaily.com/releases/2004/11/041123103713.htm (accessed September 22, 2014).

Share This



More Health & Medicine News

Monday, September 22, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Liberia Pleads for Help to Fight Ebola

Liberia Pleads for Help to Fight Ebola

AP (Sep. 22, 2014) Liberia's finance minister is urging the international community to quickly follow through on pledges of cash to battle Ebola. Bodies are piling up in the capital Monrovia as the nation awaits more help. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Ebola Doctor Says Border Controls Critical

Ebola Doctor Says Border Controls Critical

AP (Sep. 22, 2014) A Florida doctor who helped fight the expanding Ebola outbreak in West Africa says the disease can be stopped, but only if nations quickly step up their response and make border control a priority. (Sept. 22) Video provided by AP
Powered by NewsLook.com
Global Ebola Aid Increasing But Critics Say It's Late

Global Ebola Aid Increasing But Critics Say It's Late

Newsy (Sep. 21, 2014) More than 100 tons of medical supplies were sent to West Africa on Saturday, but aid workers say the global response is still sluggish. Video provided by Newsy
Powered by NewsLook.com
Sierra Leone in Lockdown to Control Ebola

Sierra Leone in Lockdown to Control Ebola

AP (Sep. 21, 2014) Sierra Leone residents remained in lockdown on Saturday as part of a massive effort to confine millions of people to their homes in a bid to stem the biggest Ebola outbreak in history. (Sept. 20) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins