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Lasers Improve Scientists' Understanding Of Complex Proteins

Date:
November 23, 2005
Source:
Lawrence Livermore National Laboratory
Summary:
By shooting lasers at an RNA polymerase (RNAP) and a strand of DNA, scientists have learned a critical component of how a complex protein develops.
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A transcribing T7 RNA polymerase initiation complex.
Credit: BioInfo Bank

By shooting lasers at an RNA polymerase (RNAP) and a strand of DNA, scientists have learned a critical component of how a complex protein develops.

Using a system called fluorescence resonance energy transfer (FRET) on a single molecule, a researcher at the Lawrence Livermore National Laboratory’s Physical Biosciences Institute (PBI) in collaboration with UCLA scientists found that the procedure that regulates genes in a strand of DNA is a single process.

Earlier studies done with less precision resulted in scientists believing that the beginning and end phases of RNAP copying a DNA strand into RNA were two different processes.
Using FRET, however, the recent study suggests that “there is no mechanistic difference between the start and finish,” said Ted Laurence of Livermore’s PBI.

RNAP is the molecular machine that serves as a gene transcription tool. When it attaches to a strand of DNA, RNAP transcribes genes to RNA, which then is translated into a protein.

FRET allows scientists to measure distances between two single molecules – a donor and an acceptor – using fluorescence. Molecules have to be less than 8-10 nanometers apart for a FRET to occur.

Using a laser process called ALEX (alternating laser excitation), developed by Laurence, the team looked into the energy transfer of a donor molecule on an RNAP to an acceptor molecule on a strand of DNA.

This was the first time a scientific team was able to confirm that the transcription initiation factor remains on an RNAP throughout the transcription process.

“Because this happens all in one phase, it may be that transcription is regulated even after starting,” Laurence said.

The research appears in the Nov. 11 issue of the journal Molecular Cell.

Founded in 1952, Lawrence Livermore National Laboratory has a mission to ensure national security and apply science and technology to the important issues of our time. Lawrence Livermore National Laboratory is managed by the University of California for the U.S. Department of Energy's National Nuclear Security Administration.


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The above post is reprinted from materials provided by Lawrence Livermore National Laboratory. Note: Materials may be edited for content and length.


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Lawrence Livermore National Laboratory. "Lasers Improve Scientists' Understanding Of Complex Proteins." ScienceDaily. ScienceDaily, 23 November 2005. <www.sciencedaily.com/releases/2005/11/051123080611.htm>.
Lawrence Livermore National Laboratory. (2005, November 23). Lasers Improve Scientists' Understanding Of Complex Proteins. ScienceDaily. Retrieved August 28, 2015 from www.sciencedaily.com/releases/2005/11/051123080611.htm
Lawrence Livermore National Laboratory. "Lasers Improve Scientists' Understanding Of Complex Proteins." ScienceDaily. www.sciencedaily.com/releases/2005/11/051123080611.htm (accessed August 28, 2015).

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