Featured Research

from universities, journals, and other organizations

Gene Therapy Technique: Water Droplets Produced By Electrospray Render Cells Permeable To External DNA

Date:
January 23, 2008
Source:
Wiley-Blackwell
Summary:
Genetically engineered products have become indispensable. For example, genetically modified bacteria produce human insulin. In future, gene therapy should make it possible to introduce genes into the cells of a diseased organism so that they can address deficiencies to compensate for malfunctions in the body. Japanese researchers have now developed a new method to introduce foreign (or synthetic) DNA into cells. The equipment needed is simple, inexpensive, and portable.

Genetically engineered products have become indispensable. For example, genetically modified bacteria produce human insulin. In future, gene therapy should make it possible to introduce genes into the cells of a diseased organism so that they can address deficiencies to compensate for malfunctions in the body. In order for this to work, foreign (or synthetic) DNA must be introduced into host cells, which is not exactly a trivial task. Japanese researchers have now developed a method which could represent a true alternative to conventional processes. The cells are “bombarded” with water droplets produced and accelerated by electrospray.

Related Articles


There are several methods to transfer DNA into a host cell. In the simplest case the foreign DNA forces its way into the cell through a cell membrane that has been made porous, through treatment with electrical current or UV lasers, for example. Viruses and liposomes can be used as genetic transporters and the genetic material can be injected or shot into the cell with a “particle gun”. These methods all have the disadvantage of either severely damaging delicate cells or of being markedly expensive and complicated.

A team at the Saitama University led by Takafumi Sakai, in cooperation with Kazuto Ikemoto (Mitsubishi Gas Chemical Company), has now developed a methodology that could provide an alternative: They “bombard” the cells with tiny electrically charged water droplets. The droplets tear tiny holes in the cell membranes, through which external DNA molecules can enter. After about one minute, the holes have closed back up and even delicate cells survive the procedure undamaged.

This method is based on a technique called electrospray, which has long been used with success, particularly in mass spectrometry. In this process, the tip of an extremely fine steel capillary is put under a high voltage. A highly charged drop of water exits the capillary and is atomized into many micro- or nanoscopic droplets. These charged microdroplets are strongly accelerated in an electrical field—toward the plate holding the cell culture.

The advantage of this new method: It is suitable for a large variety of cell types—mammalian cell cultures and bacteria, as well as living tissue, as was demonstrated with bird embryos. No cytotoxic reagents that could damage the cells are needed; only pure water or a cell-tolerated saline solution are used. An entire plate of cell cultures can be “sprayed” bit by bit, or a specific point on some tissue can be targeted. The equipment needed is simple, inexpensive, and portable.

Journal reference: DNA Introduction into Living Cells by Water Droplet Impact with an Electrospray Process. Angewandte Chemie International Edition. doi: 10.1002/anie.200704429


Story Source:

The above story is based on materials provided by Wiley-Blackwell. Note: Materials may be edited for content and length.


Cite This Page:

Wiley-Blackwell. "Gene Therapy Technique: Water Droplets Produced By Electrospray Render Cells Permeable To External DNA." ScienceDaily. ScienceDaily, 23 January 2008. <www.sciencedaily.com/releases/2008/01/080118101931.htm>.
Wiley-Blackwell. (2008, January 23). Gene Therapy Technique: Water Droplets Produced By Electrospray Render Cells Permeable To External DNA. ScienceDaily. Retrieved November 26, 2014 from www.sciencedaily.com/releases/2008/01/080118101931.htm
Wiley-Blackwell. "Gene Therapy Technique: Water Droplets Produced By Electrospray Render Cells Permeable To External DNA." ScienceDaily. www.sciencedaily.com/releases/2008/01/080118101931.htm (accessed November 26, 2014).

Share This


More From ScienceDaily



More Plants & Animals News

Wednesday, November 26, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Classic Hollywood Memorabilia Goes Under the Hammer

Classic Hollywood Memorabilia Goes Under the Hammer

Reuters - Entertainment Video Online (Nov. 26, 2014) The iconic piano from "Casablanca" and the Cowardly Lion suit from "The Wizard of Oz" fetch millions at auction. Sara Hemrajani reports. Video provided by Reuters
Powered by NewsLook.com
Pet Dogs to Be Used in Anti-Ageing Trial

Pet Dogs to Be Used in Anti-Ageing Trial

Reuters - Innovations Video Online (Nov. 26, 2014) Researchers in the United States are preparing to discover whether a drug commonly used in human organ transplants can extend the lifespan and health quality of pet dogs. Video provided by Reuters
Powered by NewsLook.com
From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

From Popcorn To Vending Snacks: FDA Ups Calorie Count Rules

Newsy (Nov. 25, 2014) The US FDA is announcing new calorie rules on Tuesday that will require everywhere from theaters to vending machines to include calorie counts. Video provided by Newsy
Powered by NewsLook.com
Feast Your Eyes: Lamb Chop Sent Into Space from UK

Feast Your Eyes: Lamb Chop Sent Into Space from UK

Reuters - Light News Video Online (Nov. 25, 2014) Take a stab at this -- stunt video shows a lamb chop's journey from an east London restaurant over 30 kilometers into space. Rough Cut (no reporter narration). Video provided by Reuters
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins