Featured Research

from universities, journals, and other organizations

Adding one single gene to yeast dramatically improves bioethanol production from agricultural waste

Date:
November 23, 2009
Source:
Delft University of Technology
Summary:
With the introduction of a single bacterial gene into yeast, researchers have achieved three improvements in bioethanol production from agricultural waste material: 'More ethanol, less acetate and elimination of the major by-product glycerol'

With the introduction of a single bacterial gene into yeast, researchers from Delft University of Technology in the Netherlands achieved three improvements in bioethanol production from agricultural waste material: 'More ethanol, less acetate and elimination of the major by-product glycerol' The invention was recently published in the scientific journal Applied and Environmental Microbiology.

Car fuel

Bioethanol is made by the yeast Saccharomyces cerevisiae from sugars obtained from plant biomass. This microorganism also converts such sugars into ethanol (alcohol) in beer and wine. The production of bioethanol is rapidly increasing due to the growing use of bioethanol as a car fuel. With an annual world production of 65 billion liters, bioethanol is aready the largest product of the fermentation industry

Second Generation

Bioethanol should of course preferably be produced from resources that do not compete with food production. For this reason, efforts are made to produce second-generation bioethanol, using agricultural residues such as wheat straw and corn stover. However, when the sugars from these raw materials are released, significant quantities of acetate are formed. Acetate can slow down or even halt bioethanol production by yeast.

Byproduct

Another challenge of the current bioethanol production process is that about 4% of the sugar is lost to formation of the byproduct glycerol. Glycerol formation was long considered to be an inevitable consequence of the production conditions during bioethanol production.

Yeast genes

TU Delft researchers have now solved these issues. Yeast can, at least in theory, also convert the harmful acetate to ethanol. As it turns out, just one single gene is missing in the yeast. By introducing a single gene from the bacterium Escherichia coli, researchers of the Netherlands-based Delft University of Technology and the Kluyver Centre for Genomics of Industrial Fermentation enabled this conversion of acetate to ethanol by yeast. This replaced the normal role of glycerol so efficiently that key genes in glycerol production could be removed, thus completely abolishing glycerol production.

Three-in-one

The invention is enthusiastically summarized by the principal researcher Jack Pronk: 'In the laboratory, this simple genetic modification kills three birds with one stone: no glycerol formation, higher ethanol yields and consumption of toxic acetate'.

For the potential billion liter ethanol gain to be realized, follow-up research on the transfer of this concept to industrial yeast strains and real-life process conditions is required. The Delft yeast researchers, who applied for a patent on their invention, hope to intensively collaborate with industrial partners to accelerate its industrial implementation.


Story Source:

The above story is based on materials provided by Delft University of Technology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Guadalupe Medina et al. Elimination of glycerol production in anaerobic cultures of Saccharomyces cerevisiae engineered for use of acetic acid as electron acceptor. Applied and Environmental Microbiology, 2009; DOI: 10.1128/AEM.01772-09

Cite This Page:

Delft University of Technology. "Adding one single gene to yeast dramatically improves bioethanol production from agricultural waste." ScienceDaily. ScienceDaily, 23 November 2009. <www.sciencedaily.com/releases/2009/11/091120084617.htm>.
Delft University of Technology. (2009, November 23). Adding one single gene to yeast dramatically improves bioethanol production from agricultural waste. ScienceDaily. Retrieved September 24, 2014 from www.sciencedaily.com/releases/2009/11/091120084617.htm
Delft University of Technology. "Adding one single gene to yeast dramatically improves bioethanol production from agricultural waste." ScienceDaily. www.sciencedaily.com/releases/2009/11/091120084617.htm (accessed September 24, 2014).

Share This



More Matter & Energy News

Wednesday, September 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Will Living Glue Be A Thing?

Will Living Glue Be A Thing?

Newsy (Sep. 23, 2014) Using proteins derived from mussels, engineers at MIT have made a supersticky underwater adhesive. They're now looking to make "living glue." Video provided by Newsy
Powered by NewsLook.com
Company Copies Keys From Photos

Company Copies Keys From Photos

Newsy (Sep. 22, 2014) A new company allows customers to make copies of keys by simply uploading a couple of photos. But could it also be great for thieves? Video provided by Newsy
Powered by NewsLook.com
The Hyped-Up Big Bang Discovery Has A Dust Problem

The Hyped-Up Big Bang Discovery Has A Dust Problem

Newsy (Sep. 22, 2014) An analysis of new satellite data casts serious doubt on a previous study about the Big Bang that was once hailed as revolutionary. Video provided by Newsy
Powered by NewsLook.com
Rockefeller Oil Heirs Switching To Clean Energy

Rockefeller Oil Heirs Switching To Clean Energy

Newsy (Sep. 22, 2014) The Rockefellers — heirs to an oil fortune that made the family name a symbol of American wealth — are switching from fossil fuels to clean energy. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Space & Time

Matter & Energy

Computers & Math

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins