Featured Research

from universities, journals, and other organizations

A better test for a potato pest

Date:
August 24, 2011
Source:
USDA/Agricultural Research Service
Summary:
Scientist have created a new weapon in the war being waged against the potato cyst nematode -- a diagnostic test that identifies the type of nematode infesting a grower's field.

ARS scientists have developed a new diagnostic test that identifies the type of potato nematode infesting a field, providing much better guidance for growers when it comes to response.
Credit: Lynn Carta

A U.S. Department of Agriculture (USDA) scientist has created a new weapon in the war being waged against the potato cyst nematode-a diagnostic test that identifies the type of nematode infesting a grower's field.

Xiaohong Wang, a molecular biologist with the Agricultural Research Service (ARS) Robert W. Holley Center for Agriculture and Health in Ithaca, N.Y., has filed a patent application on the monitoring tool, developed in part by cloning and sequencing key genes. ARS is USDA's principal intramural scientific research agency. This research supports the USDA priorities of ensuring food safety and promoting international food security.

There are two types of potato cyst nematode (PCN), the golden nematode and the pale cyst nematode. Being able to tell one from the other is important because breeders have developed potatoes that can resist the golden nematode, but have yet to develop varieties that resist the pale cyst nematode. If the pale cyst nematode is found in a field, potatoes cannot be grown there.

The golden nematode (Globodera rostochiensis) has been a problem in New York State since 1941 and has been found in Canada. The pale cyst nematode (G. pallida) was discovered in Idaho in 2006 and remains a major threat in Europe. Potatoes and seed potatoes are freely exchanged across international boundaries, so monitoring potato growing regions is essential.

Traditional methods of distinguishing between the two PCN species have relied on time-consuming morphological analyses and PCR (polymerase chain reaction) assays. They also require relatively large samples of nematode cysts. But Wang and her colleagues cloned the parasitism gene the nematodes use to produce a protein that plays an important role in the infection process, known as chorismate mutase.

The researchers then sequenced those chorismate mutase genes, compared the sequences, and identified unique regions in each sequence. They then developed a probe capable of recognizing the unique regions in each nematode's DNA. Wang described the process in a paper in the European Journal of Plant Pathology.

The diagnostic test is one of several new technologies designed to distinguish PCN types from each other, but it is a thousand times more sensitive than other systems and is expected to be widely used in regulatory and quarantine programs because it can give reliable results from tiny amounts of nematode material.


Story Source:

The above story is based on materials provided by USDA/Agricultural Research Service. The original article was written by Dennis O'Brien. Note: Materials may be edited for content and length.


Cite This Page:

USDA/Agricultural Research Service. "A better test for a potato pest." ScienceDaily. ScienceDaily, 24 August 2011. <www.sciencedaily.com/releases/2011/08/110823130027.htm>.
USDA/Agricultural Research Service. (2011, August 24). A better test for a potato pest. ScienceDaily. Retrieved July 30, 2014 from www.sciencedaily.com/releases/2011/08/110823130027.htm
USDA/Agricultural Research Service. "A better test for a potato pest." ScienceDaily. www.sciencedaily.com/releases/2011/08/110823130027.htm (accessed July 30, 2014).

Share This




More Plants & Animals News

Wednesday, July 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Concern Grows Over Worsening Ebola Crisis

Concern Grows Over Worsening Ebola Crisis

AFP (July 30, 2014) Pan-African airline ASKY has suspended all flights to and from the capitals of Liberia and Sierra Leone amid the worsening Ebola health crisis, which has so far caused 672 deaths in Guinea, Liberia and Sierra Leone. Duration: 00:43 Video provided by AFP
Powered by NewsLook.com
Raw: Otters Enjoy Water Slides at Japan Zoo

Raw: Otters Enjoy Water Slides at Japan Zoo

AP (July 30, 2014) River otters were hitting the water slides to beat the summer heatwave on Wednesday at Ichikawa City's Zoological and Botanical Garden. (July 30) Video provided by AP
Powered by NewsLook.com
In Virginia, the Rise of a New Space Coast

In Virginia, the Rise of a New Space Coast

AP (July 30, 2014) Every summer, tourists make the pilgrimage to Chincoteague Island, Va. to see wild ponies cross the Assateague Channel. But, it's the rockets sending to supplies to the International Space Station that are making this a year-round destination. (July 30) Video provided by AP
Powered by NewsLook.com
Rodents Rampant in Gardens Around Louvre

Rodents Rampant in Gardens Around Louvre

AP (July 29, 2014) Food scraps and other items left on the grounds by picnickers brings unwelcome visitors to the grounds of the world famous and popular Louvre Museum in Paris. (July 29) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins