Featured Research

from universities, journals, and other organizations

How muscle cells seal their membranes

Date:
March 14, 2012
Source:
Karlsruhe Institute of Technology
Summary:
Every cell is enclosed by a thin double layer of lipids that separates the distinct internal environment of the cell from the extracellular space. Damage to this lipid bilayer, also referred to as plasma membrane, disturbs the cellular functions and may lead to the death of the cell. For example, downhill walking tears many little holes into the plasma membranes of the muscle cells in our legs. To prevent irreparable damage, muscle cells have efficient systems to seal these holes again. Researchers have succeeded for the first time in observing membrane repair in real-time in a living organism.

Repair of the plasma membrane of a cell: For the first time, researchers have observed the relevant repair mechanisms in zebrafish.
Credit: Institute of Toxicology and Genetics, KIT

Every cell is enclosed by a thin double layer of lipids that separates the distinct internal environment of the cell from the extracellular space. Damage to this lipid bilayer, also referred to as plasma membrane, disturbs the cellular functions and may lead to the death of the cell. For example, downhill walking tears many little holes into the plasma membranes of the muscle cells in our legs. To prevent irreparable damage, muscle cells have efficient systems to seal these holes again. Researchers at Karlsruhe Institute of Technology (KIT) and Heidelberg University have succeeded for the first time in observing membrane repair in real-time in a living organism.

The results were published in the recent issue of the journal Developmental Cell. Using a novel high-resolution imaging method, Prof. Uwe Strähle and Dr. Urmas Roostalu for the first time observed membrane repair in real time in a living animal. They tagged repair proteins with fluorescent proteins in muscle of the transparent zebrafish larvae. With a laser, the researchers burned tiny holes into the plasma membrane of muscle cells and followed the repair of the holes under the microscope. They showed that membrane vesicles together with two proteins Dysferlin and Annexin A6 rapidly form a repair patch. Other Annexins accumulate subsequently on the injured membrane. These studies by Karlsruhe and Heidelberg researchers suggest that the cell assembles a multilayered repair patch from the inside that seals off the cell's interior from the extracellular environment. Moreover, it was found that there is a specialized membrane area that supplies rapidly the membrane that is needed for sealing the plasma membrane hole.

This animal model for membrane repair will contribute to the identification of new proteins in this sealing process and will help elucidate the underlying mechanisms. The results may contribute to the development of therapies for human myopathies and open up new possibilities in biotechnology.


Story Source:

The above story is based on materials provided by Karlsruhe Institute of Technology. Note: Materials may be edited for content and length.


Journal Reference:

  1. Urmas Roostalu, Uwe Strähle. In Vivo Imaging of Molecular Interactions at Damaged Sarcolemma. Developmental Cell, 2012; 22 (3): 515 DOI: 10.1016/j.devcel.2011.12.008

Cite This Page:

Karlsruhe Institute of Technology. "How muscle cells seal their membranes." ScienceDaily. ScienceDaily, 14 March 2012. <www.sciencedaily.com/releases/2012/03/120314100556.htm>.
Karlsruhe Institute of Technology. (2012, March 14). How muscle cells seal their membranes. ScienceDaily. Retrieved September 16, 2014 from www.sciencedaily.com/releases/2012/03/120314100556.htm
Karlsruhe Institute of Technology. "How muscle cells seal their membranes." ScienceDaily. www.sciencedaily.com/releases/2012/03/120314100556.htm (accessed September 16, 2014).

Share This



More Plants & Animals News

Tuesday, September 16, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Some Tobacco Farmers Thrive Amid Challenges

Some Tobacco Farmers Thrive Amid Challenges

AP (Sep. 16, 2014) — The South's tobacco country is surviving, and even thriving in some cases, as demand overseas keeps growers in the fields of one of America's oldest cash crops. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Scientists Given Rare Glimpse of 350-Kilo Colossal Squid

Scientists Given Rare Glimpse of 350-Kilo Colossal Squid

AFP (Sep. 16, 2014) — Scientists say a female colossal squid weighing an estimated 350 kilograms (770 lbs) and thought to be only the second intact specimen ever found was carrying eggs when discovered in the Antarctic. Duration: 00:47 Video provided by AFP
Powered by NewsLook.com
Raw: Scientists Examine Colossal Squid

Raw: Scientists Examine Colossal Squid

AP (Sep. 16, 2014) — Squid experts in New Zealand thawed and examined an unusual catch on Tuesday: a colossal squid. It was captured in Antarctica's remote Ross Sea in December last year and has been frozen for eight months. (Sept. 16) Video provided by AP
Powered by NewsLook.com
Conservationists Face Uphill PR Battle With New Shark Rules

Conservationists Face Uphill PR Battle With New Shark Rules

Newsy (Sep. 14, 2014) — New conservation measures for shark fishing face an uphill PR battle in the fight to slow shark extinction. Video provided by Newsy
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

    Environment News

      Technology News



      Save/Print:
      Share:  

      Free Subscriptions


      Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

      Get Social & Mobile


      Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

      Have Feedback?


      Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
      Mobile iPhone Android Web
      Follow Facebook Twitter Google+
      Subscribe RSS Feeds Email Newsletters
      Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins