Featured Research

from universities, journals, and other organizations

New 1-step process for designer bacteria

Date:
May 28, 2013
Source:
University of Adelaide
Summary:
A simpler and faster way of producing designer bacteria used in biotechnology processes has been developed.

A simpler and faster way of producing designer bacteria used in biotechnology processes has been developed by University of Adelaide researchers.

The researchers have developed a new one-step bacterial genetic engineering process called 'clonetegration', published in the journal ACS Synthetic Biology.

Led by Dr Keith Shearwin, in the University's School of Molecular and Biomedical Sciences, the research facilitates faster development of designer bacteria used in therapeutic drug development, such as insulin, and other biotechnology products.

Designer bacteria are produced by integrating extra pieces of genetic material into the DNA of bacteria, in this case E. coli, so that the bacteria will make a desired product.

"E. coli strains are commonly used workhorses for biotechnology and metabolic engineering," Dr Shearwin says.

"For example, new genes or even the genetic material for whole metabolic pathways are inserted into the bacteria's chromosome so that they produce compounds or proteins not normally produced. Insulin is an example of a therapeutic product produced in this way."

"The existing process for integrating new genes is inefficient, taking several days. Our new process can be completed overnight."

As well as speeding up the process, 'clonetegration' enables multiple rounds of genetic engineering on the same bacteria, and simultaneous integration of multiple genes at different specific locations.

"This will become a valuable technique for facilitating genetic engineering with sequences that are difficult to clone as well as enable the rapid construction of synthetic biological systems," Dr Shearwin says.

The research was a collaboration with Stanford University, California. The molecular tools needed for the clonetegration process will be made freely available for ongoing research and development.


Story Source:

The above story is based on materials provided by University of Adelaide. Note: Materials may be edited for content and length.


Journal Reference:

  1. Franηois St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, Keith E. Shearwin. One-Step Cloning and Chromosomal Integration of DNA. ACS Synthetic Biology, 2013; 130520162719006 DOI: 10.1021/sb400021j

Cite This Page:

University of Adelaide. "New 1-step process for designer bacteria." ScienceDaily. ScienceDaily, 28 May 2013. <www.sciencedaily.com/releases/2013/05/130528100238.htm>.
University of Adelaide. (2013, May 28). New 1-step process for designer bacteria. ScienceDaily. Retrieved July 24, 2014 from www.sciencedaily.com/releases/2013/05/130528100238.htm
University of Adelaide. "New 1-step process for designer bacteria." ScienceDaily. www.sciencedaily.com/releases/2013/05/130528100238.htm (accessed July 24, 2014).

Share This




More Plants & Animals News

Thursday, July 24, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Dogs Appear To Become Jealous Of Owners' Attention

Dogs Appear To Become Jealous Of Owners' Attention

Newsy (July 23, 2014) — A U.C. San Diego researcher says jealousy isn't just a human trait, and dogs aren't the best at sharing the attention of humans with other dogs. Video provided by Newsy
Powered by NewsLook.com
Professor Creates Site Revealing Where People's Cats Live

Professor Creates Site Revealing Where People's Cats Live

Newsy (July 23, 2014) — ​It's called I Know Where Your Cat Lives, and you can keep hitting the "Random Cat" button to find more real cats all over the world. Video provided by Newsy
Powered by NewsLook.com
Stone Fruit Listeria Scare Causes Sweeping Recall

Stone Fruit Listeria Scare Causes Sweeping Recall

Newsy (July 22, 2014) — The Wawona Packing Company has issued a voluntary recall on the stone fruit it distributes due to a possible Listeria outbreak. Video provided by Newsy
Powered by NewsLook.com
Michigan Plant's Goal: Flower and Die

Michigan Plant's Goal: Flower and Die

AP (July 22, 2014) — An 80-year-old agave plant, which is blooming for the first and only time at a University of Michigan conservatory, will die when it's done (July 22) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
 
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:  

Breaking News:
from the past week

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:  

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile iPhone Android Web
Follow Facebook Twitter Google+
Subscribe RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins