Featured Research

from universities, journals, and other organizations

Novel approach to gene regulation can activate multiple genes simultaneously

Date:
August 27, 2013
Source:
Whitehead Institute for Biomedical Research
Summary:
By creating a powerful new gene regulation system called CRISPR-on, researchers now have the ability to increase the expression of multiple genes simultaneously and precisely manipulate each gene’s expression level. The system is effective in both mouse and human cells as well as in mouse embryos.

By creating a powerful new gene regulation system called CRISPR-on, Whitehead Institute researchers now have the ability to increase the expression of multiple genes simultaneously and precisely manipulate each gene's expression level. The system is effective in both mouse and human cells as well as in mouse embryos.

Related Articles


"CRISPR-on is a tool that will be very useful for studying many biological processes, particularly for studying gene functions and gene networks," says Whitehead Founding Member Rudolf Jaenisch. "In contrast to RNA interference, which is commonly used to inactivate gene activity, the CRISPR-on system allows activation of cellular genes. The technology substantially expands our ability to change gene expression in cultured cells and animals.."

The system, called CRISPR-on, is a modified version of CRISPR/Cas (for "clustered regularly interspaced short palindromic repeat/CRISPR associated"), which taps into a bacterial defense system against viral intruders. CRISPR/Cas relies on an enzyme, Cas9, which cuts DNA at locations specified by single guide RNAs (sgRNAs). For CRISPR-on, the Whitehead team modified the Cas9 enzyme by eliminating its ability to cleave DNA and adding a transcription activation domain. The resulting enzyme can increase gene expression without permanently changing the DNA.

The new system is described this week in the journal Cell Research.

CRISPR-on's ability to activate only the desired genes at varying levels could be used to help scientists improve our understanding of transcription network underlying a variety of diseases and potentially find new ways to treat them.

"Many diseases, especially complex diseases, involve multiple genes, and this system could be used therapeutically to target and activate multiple genes together and rescue these disease phenotypes," says Albert Cheng, a graduate student in the Jaenisch lab and co-author of the Cell Research paper. "Or we could use it to study the gene networks in diseases and get a better understanding of how those diseases work."

So far, the researchers have used CRISPR-on to activate up to three native genes concurrently in human cells.

"I think we need to do more work to see if there are any limitations to the number of genes CRISPR-on can activate at a time," says Haoyi Wang, a co-author and postdoctoral researcher in the Jaenisch lab. "We'd like to see if we can get data on activating 10 or more genes, to see if there is an upper limit to what this system can do."

This work is supported by the Croucher Foundation and National Institutes of Health (NIH) grants HD 045022 and R37CA084198.


Story Source:

The above story is based on materials provided by Whitehead Institute for Biomedical Research. The original article was written by Nicole Giese Rura. Note: Materials may be edited for content and length.


Journal Reference:

  1. Albert W. Cheng, Haoyi Wang, Hui Yang, Linyu Shi, Yarden Katz, Sudharshan Rangarajan, Thorold W. Theunissen, Chikdu S. Shivalila, Daniel B. Dadon, Rudolf Jaenisch. RNA-guided multiplexed endogenous gene activation. Cell Research, 2013 (in press)

Cite This Page:

Whitehead Institute for Biomedical Research. "Novel approach to gene regulation can activate multiple genes simultaneously." ScienceDaily. ScienceDaily, 27 August 2013. <www.sciencedaily.com/releases/2013/08/130827091358.htm>.
Whitehead Institute for Biomedical Research. (2013, August 27). Novel approach to gene regulation can activate multiple genes simultaneously. ScienceDaily. Retrieved April 18, 2015 from www.sciencedaily.com/releases/2013/08/130827091358.htm
Whitehead Institute for Biomedical Research. "Novel approach to gene regulation can activate multiple genes simultaneously." ScienceDaily. www.sciencedaily.com/releases/2013/08/130827091358.htm (accessed April 18, 2015).

Share This


More From ScienceDaily



More Health & Medicine News

Saturday, April 18, 2015

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

Our Love Of Puppy Dog Eyes Explained By Science

Our Love Of Puppy Dog Eyes Explained By Science

Newsy (Apr. 17, 2015) Researchers found a spike in oxytocin occurs in both humans and dogs when they gaze into each other&apos;s eyes. Video provided by Newsy
Powered by NewsLook.com
Scientists Find Link Between Gestational Diabetes And Autism

Scientists Find Link Between Gestational Diabetes And Autism

Newsy (Apr. 17, 2015) Researchers who analyzed data from over 300,000 kids and their mothers say they&apos;ve found a link between gestational diabetes and autism. Video provided by Newsy
Powered by NewsLook.com
Video Messages Help Reassure Dementia Patients

Video Messages Help Reassure Dementia Patients

AP (Apr. 17, 2015) Family members are prerecording messages as part of a unique pilot program at the Hebrew Home in New York. The videos are trying to help victims of Alzheimer&apos;s disease and other forms of dementia break through the morning fog of forgetfulness. (April 17) Video provided by AP
Powered by NewsLook.com
Boy or Girl? Intersex Awareness Is on the Rise

Boy or Girl? Intersex Awareness Is on the Rise

AP (Apr. 17, 2015) At least 1 in 5,000 U.S. babies are born each year with intersex conditions _ ambiguous genitals because of genetic glitches or hormone problems. Secrecy and surgery are common. But some doctors and activists are trying to change things. (April 17) Video provided by AP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Health & Medicine

Mind & Brain

Living & Well

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins