Featured Research

from universities, journals, and other organizations

Researchers engineer better method for delivering genetic material into cells

Date:
December 16, 2013
Source:
Polytechnic Institute of New York University
Summary:
Researchers have developed a carrier in their lab that is five times more efficient in delivering DNA into cells than today's commercial delivery methods -- reagent vectors. This novel complex is a peptide-polymer hybrid, assembled from two separate, less effective vectors that are used to carry DNA into cells. The study helps researchers better understand gene function.

Researchers at the Polytechnic Institute of New York University (NYU-Poly) and the NYU College of Dentistry (NYUCD) have developed a carrier in their lab that is five times more efficient in delivering DNA into cells than today's commercial delivery methods -- reagent vectors. This novel complex is a peptide-polymer hybrid, assembled from two separate, less effective vectors that are used to carry DNA into cells.

Results of their study, "Long Term Efficient Gene Delivery Using Polyethylenimine with Modified Tat Peptide," were published in Biomaterials. The findings were the result of a collaborative research project conducted by Dr. Seiichi Yamano at NYUCD and Dr. Jin Montclare at NYU-Poly. The outcome of the study could help researchers better understand gene function and ultimately improve gene therapy.

Non-viral vectors such as those engineered in this study are used for transfection -- the process of introducing foreign genetic material (in this case, DNA called a plasmid) into a cell. The vectors are essentially vehicles that carry the genetic matter into the cell. But transfection is not as easy. Cells are set up to keep things out of the nucleus. Even if the transported plasmid manages to permeate the cellular membrane, the cytoplasm within the cell has safeguards to stop anything from getting into the nucleus.

Traditionally, scientists have engineered viruses to carry out transfection, but viruses are problematic because cells recognize them as foreign and trigger the immune response. Virus transfection is extremely costly and presents numerous difficulties for mass processing. On the other hand, non-viral vectors do not trigger the immune system and are easily manufactured and modified for safe, more effective delivery. Their shortcoming is that they generally are effective only for short periods in transfection, as well as other forms of gene expression.

For this project, Yamano and Montclare paired a modified version of CPP HIV-1 (mTat) with PEI -- a non-viral vector particularly effective for delivering oligonucleotides. In combining mTat and PEI, they built a new non-viral vector, more effective than mTat or PEI individually. They tested their reagent vector both in vitro -- grown in a Petri dish -- as well as for approximately seven months in a living organism -- in vivo.

The vector may be used in the future for targeted gene therapy.


Story Source:

The above story is based on materials provided by Polytechnic Institute of New York University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Seiichi Yamano, Jisen Dai, Shigeru Hanatani, Ken Haku, Takuto Yamanaka, Mika Ishioka, Tadahiro Takayama, Carlo Yuvienco, Sachin Khapli, Amr M. Moursi, Jin K. Montclare. Long-term efficient gene delivery using polyethylenimine with modified Tat peptide. Biomaterials, 2014; 35 (5): 1705 DOI: 10.1016/j.biomaterials.2013.11.012

Cite This Page:

Polytechnic Institute of New York University. "Researchers engineer better method for delivering genetic material into cells." ScienceDaily. ScienceDaily, 16 December 2013. <www.sciencedaily.com/releases/2013/12/131216155012.htm>.
Polytechnic Institute of New York University. (2013, December 16). Researchers engineer better method for delivering genetic material into cells. ScienceDaily. Retrieved September 30, 2014 from www.sciencedaily.com/releases/2013/12/131216155012.htm
Polytechnic Institute of New York University. "Researchers engineer better method for delivering genetic material into cells." ScienceDaily. www.sciencedaily.com/releases/2013/12/131216155012.htm (accessed September 30, 2014).

Share This



More Plants & Animals News

Tuesday, September 30, 2014

Featured Research

from universities, journals, and other organizations


Featured Videos

from AP, Reuters, AFP, and other news services

California University Designs Sustainable Winery

California University Designs Sustainable Winery

Reuters - US Online Video (Sep. 27, 2014) Amid California's worst drought in decades, scientists at UC Davis design a sustainable winery that includes a water recycling system. Vanessa Johnston reports. Video provided by Reuters
Powered by NewsLook.com
Argentina Worries Over Decline of Soybean Prices

Argentina Worries Over Decline of Soybean Prices

AFP (Sep. 27, 2014) The drop in price of soy on the international market is a cause for concern in Argentina, as soybean exports are a major source of income for Latin America's third largest economy. Duration: 01:10 Video provided by AFP
Powered by NewsLook.com
Mama Bear, Cubs Hang out in California Backyard

Mama Bear, Cubs Hang out in California Backyard

Reuters - US Online Video (Sep. 27, 2014) A mama bear and her two cubs climb trees, wrestle and take naps in the backyard of a Monrovia, California home. Vanessa Johnston reports. Video provided by Reuters
Powered by NewsLook.com
'Crazy' Climate Forces Colombian Farmers to Adapt

'Crazy' Climate Forces Colombian Farmers to Adapt

AFP (Sep. 26, 2014) Once upon a time, farming was a blissfully low-tech business on Colombia's northern plains. Duration: 02:05 Video provided by AFP
Powered by NewsLook.com

Search ScienceDaily

Number of stories in archives: 140,361

Find with keyword(s):
Enter a keyword or phrase to search ScienceDaily for related topics and research stories.

Save/Print:
Share:

Breaking News:

Strange & Offbeat Stories


Plants & Animals

Earth & Climate

Fossils & Ruins

In Other News

... from NewsDaily.com

Science News

Health News

Environment News

Technology News



Save/Print:
Share:

Free Subscriptions


Get the latest science news with ScienceDaily's free email newsletters, updated daily and weekly. Or view hourly updated newsfeeds in your RSS reader:

Get Social & Mobile


Keep up to date with the latest news from ScienceDaily via social networks and mobile apps:

Have Feedback?


Tell us what you think of ScienceDaily -- we welcome both positive and negative comments. Have any problems using the site? Questions?
Mobile: iPhone Android Web
Follow: Facebook Twitter Google+
Subscribe: RSS Feeds Email Newsletters
Latest Headlines Health & Medicine Mind & Brain Space & Time Matter & Energy Computers & Math Plants & Animals Earth & Climate Fossils & Ruins