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Researchers Develop MRI Technique To Study Brain Anatomy In Invertebrates

Date:
December 29, 2004
Source:
Georgia State University
Summary:
Scientists with the Center for Behavioral Neuroscience, a research consortium based at Georgia State University, have for the first time used a form of magnetic resonance imaging to reveal anatomical features of the nervous system in a live crayfish, a crustacean whose brain measures only 3 millimeters wide.
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ATLANTA -- Scientists with the Center for Behavioral Neuroscience, a research consortium based at Georgia State University, have for the first time used a form of magnetic resonance imaging to reveal anatomical features of the nervous system in a live crayfish, a crustacean whose brain measures only 3 millimeters wide.

The technique, which is reported and highlighted in an accompanying commentary in the Dec. 15 issue of The Journal of Experimental Biology, provides a powerful new tool for understanding the neurobiology of behavior in invertebrate animal models.

Conventional MRI technology employs high-intensity magnetic fields to excite protons in the water molecules of soft tissue. Scanners detect the excitation and image cross-sectional slices of an organ, such as the brain. To image a live crayfish, whose physiology does not normally respond to magnetic fields, CBN researchers injected manganese, a contrast-enhancing agent that crayfish cells absorb, through a long tube into its circulatory system. The infusion took place while the animal was positioned inside the MRI scanner.

"Before injecting the manganese, we couldn't see the animal's brain at all," said Georgia State research scientist Jens Herberholz, the study's lead author. "But shortly after injecting it, we suddenly saw the brain light up like a Christmas tree and could easily discern its substructures."

Herberholz and his colleagues, including Georgia State professor Donald Edwards, Georgia State lab technician Christopher Mims, and Emory University's Xiaodong Zhang and Xiaoping Hu, developed manganese-enhanced MRI to study the crayfish brain. They discovered the technique also is effective in imaging other parts of the animal's body. In their pilot study, the scientists imaged a number of complex anatomical structures in the foregut that had never been seen in a live crayfish.

Crayfish serve as one of the best animal models for studying the neural bases of aggression and social hierarchies. During an initial encounter, two crayfish fight one another to establish dominant and subordinate roles. This interaction alters the physiology of each animal's brain at the cellular level.

Before manganese-enhanced MRI, researchers relied on dissections and electrophysiology to measure the neural changes associated with social behavior in crayfish. With the new technology, which is not harmful to crayfish, Herberholz said the same animal could be imaged repeatedly, enabling scientists to capture changes as they occur.

"This technique can be used to study invertebrate anatomy, but manganese also is a marker for neural activity," said Herberholz. "There are a wealth of potential applications for research on invertebrates, which serve as important model systems for understanding how neural circuitry produces behavior in all animals, including humans."

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The Center for Behavioral Neuroscience, a National Science Foundation Science and Technology Center consisting of more than 90 neuroscientists at eight metro Atlanta colleges and universities, conducts research on the basic neurobiology of complex social behaviors. Its programs have led to a breakthrough treatment for anxiety-related disorders and new understanding of the potential roles of the neurochemicals vasopressin and oxytocin in autism. CBN's workforce training programs also have contributed significantly to enhancing the diversity of Georgia's burgeoning life sciences industry. In addition to research and education, CBN also develops technologies for behavioral neuroscience.


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Materials provided by Georgia State University. Note: Content may be edited for style and length.


Cite This Page:

Georgia State University. "Researchers Develop MRI Technique To Study Brain Anatomy In Invertebrates." ScienceDaily. ScienceDaily, 29 December 2004. <www.sciencedaily.com/releases/2004/12/041219181540.htm>.
Georgia State University. (2004, December 29). Researchers Develop MRI Technique To Study Brain Anatomy In Invertebrates. ScienceDaily. Retrieved December 4, 2024 from www.sciencedaily.com/releases/2004/12/041219181540.htm
Georgia State University. "Researchers Develop MRI Technique To Study Brain Anatomy In Invertebrates." ScienceDaily. www.sciencedaily.com/releases/2004/12/041219181540.htm (accessed December 4, 2024).

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