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New enzyme reduces sulfite in wine even faster

Date:
February 3, 2015
Source:
Albert-Ludwigs-Universität Freiburg
Summary:
Sulfites are sulfurous substances that occur naturally. They are poisonous for many life forms even at small concentrations. Sulfites and sulfur dioxide are also added to wine and dried fruit as preservatives that inhibit the growth of unwanted microorganisms, increasing the shelf-life of these products. Biochemists have now characterized a bacterial enzyme that reduces sulfite up to one hundred times faster than any other known enzyme.
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The enzyme complex MccA contains ion and copper ions.
Credit: : AG Einsle

Sulfites are sulfurous substances that occur naturally. They are poisonous for many life forms even at small concentrations. Sulfites and sulfur dioxide are also added to wine and dried fruit as preservatives that inhibit the growth of unwanted microorganisms, increasing the shelf-life of these products. The biochemists Prof. Dr. Oliver Einsle and Dr. Bianca Hermann from the University of Freiburg have teamed up with researchers from the Technische Universität Darmstadt for a project in which they characterised a bacterial enzyme that reduces sulfite up to one hundred times faster than any other known enzyme. The researchers were able to clarify the high-resolution crystal structure of the enzyme complex MccA and the molecular details of its reaction mechanism.

Their findings explain why MccA is able to convert sulfite so quickly, meaning that this enzyme could be used more frequently in biotechnology in the future. Customised microorganisms capable of high-speed sulfite reduction could be employed for desulfurization under mild conditions, for example. The results of the scientists' research have now been published in Nature.

Some bacteria use sulfite for their metabolism -- for example, for respiration when there is no oxygen. Bacteria harvest energy by reducing sulfite to sulfide, during which they split off all oxygen atoms as water. One of the bacteria that use sulfur for respiration is Wolinella succinogenes. This organism can be found in the rumen of ruminants, where it produces MccA. MccA is a metalloprotein that contains 24 haem groups with iron ions. It also consists of three subunits, making it a trimer.

The scientists were able to decode the enzyme's structure, revealing a previously unknown active centre for sulfite reduction. This centre consists of one of the haem groups combined with a copper ion, which is bound to two residues of cysteine, a sulfurous amino acid. Through its position, the copper ion prevents sulfite ions from binding to the enzyme. Instead, sulfur dioxide binds to the active centre because it formally contains one less water molecule and thus requires less space, so to say. This initial step is what distinguishes the reduction mechanism of MccA from other enzymes that break down sulfite. MccA then reduces sulfur dioxide to sulfide through further dehydration. The scientists were able to detect sulfur dioxide and sulfur monoxide in the enzyme's structural model, from which they concluded this new mechanism.

Einsle is head of the Membrane Proteins and Metalloproteins Lab at the Institute of Biochemistry at the University of Freiburg and an associated member of the cluster of excellence BIOSS Centre for Biological Signalling Studies, also at the University of Freiburg. Hermann is a postdoctoral researcher at the Institute of Biochemistry and a member of Einsle's lab team.


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Materials provided by Albert-Ludwigs-Universität Freiburg. Note: Content may be edited for style and length.


Journal Reference:

  1. Bianca Hermann, Melanie Kern, Luigi La Pietra, Jörg Simon, Oliver Einsle. The octahaem MccA is a haem c–copper sulfite reductase. Nature, 2015; DOI: 10.1038/nature14109

Cite This Page:

Albert-Ludwigs-Universität Freiburg. "New enzyme reduces sulfite in wine even faster." ScienceDaily. ScienceDaily, 3 February 2015. <www.sciencedaily.com/releases/2015/02/150203112022.htm>.
Albert-Ludwigs-Universität Freiburg. (2015, February 3). New enzyme reduces sulfite in wine even faster. ScienceDaily. Retrieved May 26, 2017 from www.sciencedaily.com/releases/2015/02/150203112022.htm
Albert-Ludwigs-Universität Freiburg. "New enzyme reduces sulfite in wine even faster." ScienceDaily. www.sciencedaily.com/releases/2015/02/150203112022.htm (accessed May 26, 2017).

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