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Non-Hodgkin's lymphoma: Rare, deadly lymphoma demystified

Date:
April 14, 2015
Source:
Columbia University Medical Center
Summary:
The first-ever systematic study of the genomes of patients with ALK-negative anaplastic large cell lymphoma, a particularly aggressive form of non-Hodgkin's lymphoma, has been concluded. The findings identify a cause of the disease and highlight potential therapeutic approach to the illness.
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This is a map of mutations observed in ALK-negative anaplastic large cell lymphoma.
Credit: Dr. Raul Rabadan

The first-ever systematic study of the genomes of patients with ALK-negative anaplastic large cell lymphoma (ALCL), a particularly aggressive form of non-Hodgkin's lymphoma, shows that many cases of the disease are driven by alterations in the JAK/STAT3 cell signaling pathway. The study also demonstrates, in mice implanted with human-derived ALCL tumors, that the disease can be inhibited by compounds that target this pathway, raising hopes that more effective treatments might soon be developed. The study, led by researchers at Columbia University Medical Center (CUMC) and Weill Cornell Medical College, was published today in the online edition of Cancer Cell.

"Current therapies for this form of lymphoma fail to work in the majority of cases. However, now that we know the mutations that drive a significant percentage of cases, we can envision a new, personalized genomic approach to the treatment of ALK-negative ALCL," said co-study leader Raul Rabadan, PhD, associate professor of systems biology and of biomedical informatics at Columbia University. The other co-study leader is Giorgio Inghirami, MD, of Weill Cornell Medical College.

About 70,000 cases of non-Hodgkin's lymphoma (NHL) are diagnosed each year; ALCL accounts for about 3 percent of them. Patients with systemic ALCL (disease that has spread to multiple body sites) fall into two groups, depending on whether their cells express an abnormal form of the ALK (anaplastic lymphoma kinase) protein. ALK-positive lymphomas tend to respond well to chemotherapy, with a long-term disease-free survival rate of more than 70 percent. These lymphomas are known to result from the fusion of two genes, which produces an abnormal protein that activates a third gene, STAT3. Patients with ALK-negative lymphomas have a worse prognosis, with a long-term survival rate of less than 50 percent. Very little is known about the cause of this form of the disease.

To learn more about the genetics of ALCL, Dr. Rabadan and his colleagues sequenced the exomes (the protein-coding portion of the genomes) and the RNA of tumor cells from 155 patients with ALCL and 74 controls (patients with other forms of lymphoma). The team found mutations in either JAK1 or STAT3 in about 20 percent of the 88 patients with ALK-negative ALCL. Of those 20 percent, 38 percent had mutations in both genes.

Either JAK1or STAT3 mutations can cause abnormal activation of the JAK/STAT3 signaling pathway, which transmits chemical signals from outside the cell to genes in the cell nucleus. Overactivation of this pathway has been implicated in various forms of cancer.

The researchers also detected the presence of several novel gene fusions, some of which appear to activate the JAK/STAT3 pathway. Patients with these gene fusions did not have JAK1 or STAT3 mutations, suggesting that the fusions are an independent cause of ALK-negative ALCL.

To confirm whether JAK1 and STAT3 mutations can cause ALK-negative ALCL, the researchers induced these mutations in normal human cells. The mutations led to diseased cells.

Finally, the researchers tested JAK/STAT3 pathway inhibitors in mice implanted with tumors derived from patients with ALK-negative ALCL. Tumor growth was significantly inhibited, compared with controls. "Our findings demonstrate that drugs targeting the JAK/STAT3 pathway offer a viable therapeutic strategy in a subset of patients with ALCL," said Dr. Rabadan. "A couple of JAK/STAT3 inhibitors have been approved by the FDA for the treatment of psoriasis and rheumatoid arthritis, and several more are currently in clinical trials. These could be tested in patients whose genetic profile matches those we identified in our study."


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Materials provided by Columbia University Medical Center. Note: Content may be edited for style and length.


Journal Reference:

  1. Ramona Crescenzo, Francesco Abate, Elena Lasorsa, Fabrizio Tabbo’, Marcello Gaudiano, Nicoletta Chiesa, Filomena Di Giacomo, Elisa Spaccarotella, Luigi Barbarossa, Elisabetta Ercole, Maria Todaro, Michela Boi, Andrea Acquaviva, Elisa Ficarra, Domenico Novero, Andrea Rinaldi, Thomas Tousseyn, Andreas Rosenwald, Lukas Kenner, Lorenzo Cerroni, Alexander Tzankov, Maurilio Ponzoni, Marco Paulli, Dennis Weisenburger, Wing C. Chan, Javeed Iqbal, Miguel A. Piris, Alberto Zamo’, Carmela Ciardullo, Davide Rossi, Gianluca Gaidano, Stefano Pileri, Enrico Tiacci, Brunangelo Falini, Leonard D. Shultz, Laurence Mevellec, Jorge E. Vialard, Roberto Piva, Francesco Bertoni, Raul Rabadan, Giorgio Inghirami. Convergent Mutations and Kinase Fusions Lead to Oncogenic STAT3 Activation in Anaplastic Large Cell Lymphoma. Cancer Cell, 2015; 27 (4): 516 DOI: 10.1016/j.ccell.2015.03.006

Cite This Page:

Columbia University Medical Center. "Non-Hodgkin's lymphoma: Rare, deadly lymphoma demystified." ScienceDaily. ScienceDaily, 14 April 2015. <www.sciencedaily.com/releases/2015/04/150414160720.htm>.
Columbia University Medical Center. (2015, April 14). Non-Hodgkin's lymphoma: Rare, deadly lymphoma demystified. ScienceDaily. Retrieved May 23, 2017 from www.sciencedaily.com/releases/2015/04/150414160720.htm
Columbia University Medical Center. "Non-Hodgkin's lymphoma: Rare, deadly lymphoma demystified." ScienceDaily. www.sciencedaily.com/releases/2015/04/150414160720.htm (accessed May 23, 2017).

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