Acoustic tweezers that can move single cells in three dimensions using surface acoustic waves without touching, deforming or labeling the cells are possible, according to a team of engineers.
"In this application we use surface acoustic waves to create nodes where cells or microparticles are trapped," said Tony Jun Huang, professor and The Huck Distinguished Chair in Bioengineering Science and Mechanics. "We can then move the cell or particle in three dimensions to create structures in two or three dimensions."
The trapping nodes are formed by two sets of surface-acoustic-wave generators. When the sound waves from opposite sides meet, they create pressure that catches and positions the particle or cell. Moving the location where the sound waves meet moves the location of the cell or particle. These standing-wave shifts manipulate the tiny objects in two dimensions. The amplitude of the acoustic vibrations controls the movement in the third dimension. The researchers report their work in today's (Jan. 25) issue of the Proceedings of the National Academy of Sciences.
"The results presented in this paper provide a unique pathway to manipulate biological cells, accurately and in three dimensions, without the need for any invasive contact, tagging, or biochemical labeling," said Subra Suresh, president, Carnegie Mellon University and part of the research team. "This approach could lead to new possibilities for research and applications in such areas as regenerative medicine, neuroscience, tissue engineering, biomanufacturing, and cancer metastasis."
The research team not only created a 3-D tweezers, but they also modeled bioprinting with this device and used the device to pick up, translate and print single cells and cell assemblies, creating 2-D and 3-D structures in a precise, noninvasive manner. They demonstrated this ability by capturing a single suspended mouse fibroblast and moving it to a targeted location in the microfluidic chamber.
Bioprinting to recreate biological materials must include a way to preserve cell-to-cell communications and cell-environment interactions. While the device is not a 3-D printer in the conventional sense, it can move specific cells and particles to specified places and attach them wherever they belong in a functional way.
"Adding a third dimension for precisely manipulating single cells for bioprinting further advances acoustic tweezers technology," said Ming Dao, director, Nanomechanics Lab, Massachusetts Institute of Technology. "The accompanying modeling provides solutions for cell manipulation, enabling validation of the method as well as possible system optimization."
The third dimension achieved with this device relies on acoustic streaming, a type of fluidic motion induced by a standing acoustic wave. By manipulating the acoustic wave, the researchers could position the trapped particle or cell wherever they wanted it within the vertical confines of the enclosed fluid.
"3-D acoustic tweezers can pattern cells with control over the number of cells, cell spacing and the confined geometry, which may offer a unique way to print neuron cells to create artificial neural networks for neuron science applications or regenerative neuron medicine," said Huang.
The current device can place a cell or particle with 1 micrometer accuracy horizontally and with 2 micrometer accuracy vertically. The researchers moved a 10 micrometer particle at an average speed of about 2.5 micrometers per second and could place cells in several seconds to a few minutes depending on the distance.
Because the acoustic wavelength and input power are instantaneously tunable during experiments, the placement accuracy is only limited by the resolution of the device setup, according to the researchers.
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